Detection of Chromosomal Translocation Transcripts with Molecular Beacons

描述(由申请人提供)：这一第一阶段提案的目的是证明可以利用分子信标来检测由染色体易位引起的癌基因，这是一种在某些类型的癌症中常见的现象。分子信标技术的成功使用将使人们能够筛选单个癌细胞，从而提高检测癌症的灵敏度。商业化：专门识别癌基因的分子信标将在携带癌基因的小鼠模型和临床癌症样本中进行测试。分子信标将成为检测和诊断癌症的宝贵工具。这些研究的成功将为诊断由染色体易位引起的癌症提供一种改进的方法。分子信标技术提供的增强的灵敏度将使医生能够在早期阶段治疗癌症。

DESCRIPTION (provided by applicant): The purpose of this Phase I proposal is to demonstrate that molecular beacons can be exploited to detect oncogenes that result from chromosomal translocation, a phenomenon commonly found in some types of cancer. The success of using molecular beacon technology will enable the ability to screen for individual cancer cells and thereby increasing the sensitivity for the detection of cancer. Commercialization: The molecular beacons that specifically recognize oncogenes will be tested in mice models harboring the oncogene and in clinical cancer samples. Molecular beacons will be valuable tools for detecting and diagnosing cancer. The success of these studies will provide an improved method to diagnose cancer that is a result of chromosomal translocation. The enhanced sensitivity provided by molecular beacon technology will enable physicians to treat for cancer at earlier stages.