CROSSTALK OF AT1A RECEPTOR SIGNALING

AT1A 受体信号传导的串扰

• 批准号: 6388742
• 负责人: THANH N DOAN
• 金额: $ 4.38万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-07-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6388742
• 关键词: CHO cells; G protein; angiotensin II; angiotensin receptor; biological signal transduction; cell proliferation; gene expression; mutant; phosphorylation; protein structure function; protein tyrosine kinase; receptor coupling; tissue /cell culture; tyrosine

The AT1 receptor is important for blood pressure. The signaling mechanisms of the receptor is of particular interest in order to understand the overall effect of angiotensin 11 binding to the receptor. The proposal herein addresses: Specific Aim 1: To investigate the importance of the five tyrosines located on the carboxyl tail of the AT receptor for G-protein coupling and/or activation. Hypothesis - The tyrosines on the carboxyl tail of the AT1 receptor are necessary for -protein coupling and/or activation. Specific Aim 2: To investigate the significance of the five tyrosines located on the carboxyl tail of the AT1 receptor for tyrosine kinase activity. Hypothesis - The mutation of the five tyrosines on the carboxyl tail of the AT receptor does not disrupt the coupling of the receptor to intracellular tyrosine kinase proteins.

AT1受体对血压很重要。为了了解血管紧张素11与受体结合的整体效应，人们对受体的信号机制特别感兴趣。这里的建议涉及：具体目标1：研究位于AT受体羧基尾部的五种酪氨酸对G蛋白偶联和/或激活的重要性。假设-AT1受体羧基尾部的酪氨酸是蛋白质偶联和/或激活所必需的。具体目的2：探讨AT1受体羧基尾部的5种酪氨酸对酪氨酸激酶活性的影响。假设-AT受体羧基尾部的五个酪氨酸的突变不会破坏该受体与细胞内酪氨酸激酶蛋白的偶联。