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Real-time nucleic acids detection assay with enhanced 5'-nuclease activity

具有增强的 5-核酸酶活性的实时核酸检测分析

基本信息

批准号：
7269551
负责人：
IGOR VASSILY KUTYAVIN
金额：
$ 10万
依托单位：
PERPETUAL GENOMICS
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-05-01 至 2007-10-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7269551
关键词：
Affect Applications Grants Award Biological Assay Buffers Characteristics Clinical Complex Condition DNA DNA amplification DNA-Directed DNA Polymerase Data Deception Detection Diagnostic Disease Energy Transfer Enhancers Environment Enzymes Evaluation Evaluation Studies Fluorescence Food Gene Amplification Generations Genes Genomics Genotype Goals Grant Hand Head Housing Human Hybrids In Vitro Intercalating Agents Length Literature Location Marketing Measurement Minor Groove Modification Molecular Molecular Profiling Nuclear Nucleic Acids Nucleosides Oligonucleotide Probes Oligonucleotides Outcomes Research Performance Personal Satisfaction Phase Phase I Clinical Trials Polymerase Polymerase Chain Reaction Polymorphism Analysis Population Analysis Positioning Attribute Principal Investigator Productivity Proteomics Protocols documentation Provider Range Reaction Reagent Relative (related person)Research Running Scorpions Sensitivity and Specificity Signal Transduction Single Nucleotide Polymorphism Small Business Funding Mechanisms Small Business Innovation Research Grant Solutions Speed Staging Structure Surgical Flaps System Tail Taq Polymerase Techniques Technology Temperature Testing Time United States National Institutes of Health Work base clinically relevant cost design improved innovation instrument interest magnesium ion new technology novel nuclease nucleic acid detection nucleoside analog pathogen preference programs research study response tool

项目摘要

DESCRIPTION (provided by applicant): Simple and straightforward approach to enhance cleavage of FRET-probes has been discovered. Main objective of this Phase I study is to develop a novel real-time PCR assay for nucleic acid detection and identification. Preliminary results indicate that the technology effectively competes with other known FRET-probe systems providing strong signal performance in real-time PCR. New assay is anticipated to become a superior tool for genomic studies and, in particular, for single nucleotide polymorphism identification and genotyping. It can be effectively used in research and clinical environment for numerous applications including disease linkage study, population analysis, expression profiling and pathogen identification. Main objective of the proposed project is to develop a novel and improved real-time PCR assay for nucleic acid detection and identification. Our initial study indicates that the technology will become a superior tool in human genomics, and it can be effectively used in research and clinical environment for numerous applications including disease linkage study, population analysis, genotyping, expression profiling and pathogen identification.

描述（由申请人提供）：已经发现了增强 FRET 探针裂解的简单且直接的方法。该一期研究的主要目标是开发一种用于核酸检测和鉴定的新型实时 PCR 检测方法。初步结果表明，该技术可与其他已知的 FRET 探针系统有效竞争，在实时 PCR 中提供强大的信号性能。新的检测方法预计将成为基因组研究的优质工具，特别是单核苷酸多态性鉴定和基因分型的工具。它可以有效地用于研究和临床环境中的多种应用，包括疾病连锁研究、群体分析、表达谱和病原体鉴定。该项目的主要目标是开发一种新颖且改进的实时 PCR 检测方法，用于核酸检测和鉴定。我们的初步研究表明，该技术将成为人类基因组学的优越工具，并且可以有效地用于研究和临床环境中的众多应用，包括疾病连锁研究、群体分析、基因分型、表达谱和病原体鉴定。