Real-time nucleic acids detection assay with enhanced 5'-nuclease activity

描述（由申请人提供）：已经发现了一种简单直接的方法来增强fret探针的裂解。本一期研究的主要目的是开发一种新型的实时PCR核酸检测和鉴定方法。初步结果表明，该技术有效地与其他已知的fret探针系统竞争，在实时PCR中提供强大的信号性能。新的分析预计将成为基因组研究的优越工具，特别是单核苷酸多态性鉴定和基因分型。它可以有效地应用于研究和临床环境，包括疾病连锁研究、群体分析、表达谱和病原体鉴定。该项目的主要目标是开发一种新型和改进的实时PCR检测方法，用于核酸检测和鉴定。我们的初步研究表明，该技术将成为人类基因组学的一个优越工具，可以有效地应用于研究和临床环境，包括疾病连锁研究、群体分析、基因分型、表达谱和病原体鉴定。

DESCRIPTION (provided by applicant): Simple and straightforward approach to enhance cleavage of FRET-probes has been discovered. Main objective of this Phase I study is to develop a novel real-time PCR assay for nucleic acid detection and identification. Preliminary results indicate that the technology effectively competes with other known FRET-probe systems providing strong signal performance in real-time PCR. New assay is anticipated to become a superior tool for genomic studies and, in particular, for single nucleotide polymorphism identification and genotyping. It can be effectively used in research and clinical environment for numerous applications including disease linkage study, population analysis, expression profiling and pathogen identification. Main objective of the proposed project is to develop a novel and improved real-time PCR assay for nucleic acid detection and identification. Our initial study indicates that the technology will become a superior tool in human genomics, and it can be effectively used in research and clinical environment for numerous applications including disease linkage study, population analysis, genotyping, expression profiling and pathogen identification.