Development of new PCR detection methods based on use of exceptionally short FRET

描述（由申请人提供）： 根据我们的初步研究，提出进一步开发的是一种新的PCR系统设计，其可以允许使用极短的<6-8-mer FRET探针用于PCR后和实时PCR形式的核酸检测。该项目的目标是通过建立所谓的“通用探针库”，使FRET探针能够在市场上部分销售。“完整的文库将始终包含针对靶DNA的任何给定序列的互补探针组分。预计这将节省数百万美元的公共和私人资金，并大大加快基因组学、药物基因组学和其他保健相关学科的科学研究。在项目结束时，我们的目标是优化和完成新发现的方法的系统设计，并估计相应的通用探针库的预期大小，用于后PCR和实时应用。新的检测方法将在研究和临床环境中应用，用于疾病连锁研究、群体分析、表达谱分析和病原体鉴定。由于其无与伦比的成本效益，这项新技术有望迅速取代目前用于核酸检测和诊断的常规检测和分子工具。 公共卫生相关性： 根据我们的初步研究，提出进一步开发的是一种新的PCR系统设计，其可以允许使用极短的<6-8-mer FRET探针用于PCR后和实时PCR形式的核酸检测。新的检测方法将在研究和临床环境中应用，用于疾病连锁研究、群体分析、表达谱分析和病原体鉴定。

DESCRIPTION (provided by applicant): Proposed for further development is a new PCR system design which, according to our preliminary study, may allow the use of extremely short <6-8-mer FRET probes for nucleic acid detection in post-PCR and real-time PCR formats. The goal of this project is to enable the fractional sale of FRET-probes on the market by establishing a so-called "universal probe library." The complete library would always contain a complementary probe component for any given sequence of the target DNA. This is anticipated to save many millions of dollars of public and private money and significantly accelerate scientific research in genomics, pharmacogenomics and other healthcare-related disciplines. At the end of the project we aim to optimize and finalize the system design for the newly discovered method and estimate the anticipated sizes of the corresponding universal probe libraries for the post-PCR and real-time applications. The new assays will have application in both research and clinical settings for disease linkage studies, population analysis, expression profiling and pathogen identification. Due to its unparalleled cost effectiveness, this new technology is expected to quickly replace the conventional assays and molecular tools currently used for nucleic acid detection and diagnostics. PUBLIC HEALTH RELEVANCE: Proposed for further development is a new PCR system design which, according to our preliminary study, may allow the use of extremely short <6-8-mer FRET probes for nucleic acid detection in post-PCR and real-time PCR formats. The new assays will have application in both research and clinical settings for disease linkage studies, population analysis, expression profiling and pathogen identification.