Core--Anatomic Pathology

解剖病理学核心(核心B)将发展和协助所有外科和组织学 在计划项目赠款(PPG)的所有单个项目中描述的技术。该核心由一名负责开发和实施小鼠外科模型的高级兽医技术员、一名经验丰富的组织学家(开发和协助组织学和免疫组织化学方法)和一名初级组织学技术员(协助准备、切片和组织染色)组成。这名兽医的职责将包括开发和协助与以下相关的外科手术：(1)卡斯特利诺项目中描述的内毒素血症模型，包括放置测量血压的放射性遥测探头，切除EC制剂中的动脉和静脉，并插入渗透泵以输送脂多糖；(2)无菌地从APC[Min/o]小鼠身上切除原发肿瘤，并进行普氏项目中描述的一般尸检；(3)如Rosen项目中描述的子宫移植方案。其他职责将涉及开发与这一应用相关的新型号。组织学家的职责包括准备和切片组织以进行组织化学和免疫组织化学染色：(1)内毒素血症研究中可能受损的组织；(2)原发和转移性肿瘤以及Ploplis项目中描述的新的肿瘤细胞系；(3)子宫内移植研究中定植的供体肝细胞；以及Rosen项目中描述的受损血管壁中的血栓。其他职责将包括：(4)为所有 新菌种的研究、开发和优化。

The Anatomic Pathology Core (Core B) will develop and assist in all surgical and histological techniques described in all of the individual projects in the Program Project Grant (PPG). This Core is staffed by a senior veterinary technician, who is responsible for developing and implementing murine surgical models, an experienced histologist, who develops and assists in histological and immunohistochemical methods, and a junior histology technician, who assists in preparing, sectioning, and staining of tissue. The responsibilities of the veterinary technician will include the development and assistance in surgeries associated with: (1) endotoxemia models as described in Project by Castellino, including placements of radiotelemetry probes for BP measurements, removal of arteries and veins for EC preparations, and insertions of osmotic pumps for LPS delivery; (2) aseptic removal of the primary tumors from the APC[Min/o] mice and general necropsy described in Project by Ploplis; and (3) in utero transplantation protocols as described in Project by Rosen. Additional responsibilities will involve development of new models pertinent to this application. The responsibilities of the histologists include preparing and sectioning of tissue in order to perform histochemical and immunohistochemical stains for: (1) tissues potentially damaged in the endotoxemia studies; (2) primary and metastatic tumors as well as new tumor cell lines described in Project by Ploplis; (3) colonized donor hepatocytes in the in utero transplantation studies and clots in injured vessel walls described in Project by Rosen. Additional responsibilities will include: (4) preparation of control tissues for all the studies and development and optimization of new stains.