Core--Anatomic Pathology

核心--解剖病理学

• 批准号: 7228998
• 负责人: Victoria Ploplis
• 金额: $ 20.7万
• 依托单位: UNIVERSITY OF NOTRE DAME
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-01 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7228998
• 关键词: anatomy; biomedical facility; cell transplantation; hemostasis; histology; immunocytochemistry; in utero transplantation; laboratory mouse; model design /development; pathology; septicemia; surgery; technology /technique development; tissue /cell preparation; veterinary medicine

The Anatomic Pathology Core (Core B) will develop and assist in all surgical and histological techniques described in all of the individual projects in the Program Project Grant (PPG). This Core is staffed by a senior veterinary technician, who is responsible for developing and implementing murine surgical models, an experienced histologist, who develops and assists in histological and immunohistochemical methods, and a junior histology technician, who assists in preparing, sectioning, and staining of tissue. The responsibilities of the veterinary technician will include the development and assistance in surgeries associated with: (1) endotoxemia models as described in Project by Castellino, including placements of radiotelemetry probes for BP measurements, removal of arteries and veins for EC preparations, and insertions of osmotic pumps for LPS delivery; (2) aseptic removal of the primary tumors from the APC[Min/o] mice and general necropsy described in Project by Ploplis; and (3) in utero transplantation protocols as described in Project by Rosen. Additional responsibilities will involve development of new models pertinent to this application. The responsibilities of the histologists include preparing and sectioning of tissue in order to perform histochemical and immunohistochemical stains for: (1) tissues potentially damaged in the endotoxemia studies; (2) primary and metastatic tumors as well as new tumor cell lines described in Project by Ploplis; (3) colonized donor hepatocytes in the in utero transplantation studies and clots in injured vessel walls described in Project by Rosen. Additional responsibilities will include: (4) preparation of control tissues for all the studies and development and optimization of new stains.

解剖病理学核心（核心B）将开发并协助所有手术和组织学 技术描述的所有个人项目在计划项目补助金（PPG）。该核心由一名高级兽医技术员、一名经验丰富的组织学家和一名初级组织学技术员组成，前者负责开发和实施小鼠手术模型，后者负责开发和协助组织学和免疫组织化学方法，前者负责协助组织的制备、切片和染色。兽医技术员的职责将包括开发和协助与以下相关的手术：（1）Castellino项目中所述的内毒素血症模型，包括放置无线电遥测探头进行BP测量、去除动脉和静脉进行EC制备以及插入渗透泵输送LPS;（2）从APC[Min/o]小鼠中无菌取出原发性肿瘤并进行Ploplis的Project中所述的一般尸检;和（3）如罗森的Project中所述的子宫内移植方案。其他职责将包括开发与此应用相关的新模型。组织学家的职责包括组织制备和切片，以便对以下组织进行组织化学和免疫组织化学染色：（1）内毒素血症研究中可能受损的组织;（2）原发性和转移性肿瘤以及Ploplis项目中描述的新肿瘤细胞系;（3）子宫内移植研究中的移植肝细胞和罗森的项目中描述的损伤血管壁中的凝块。其他职责将包括：（4）为所有试验准备对照组织。 研究、开发和优化新的染色剂。