Methylation patterns in colorectal cancer

描述（由申请人提供）：在这里，我建议使用结直肠癌分子流行病学（MECC）研究，这是一项基于以色列北部结直肠癌的大型人群病例对照研究，来调查结直肠癌的甲基化状态。该建议的总体假设是，甲基化状态代表了一组独特的CRC，可以独立预测CRC的总生存率。具体来说，目标是：

DESCRIPTION (provided by applicant): Here, I propose to use the Molecular Epidemiology of Colorectal Cancer (MECC) study, a large population-based case-control study of colorectal cancer in northern Israel, to investigate methylation status in colorectal cancer. The overall hypothesis of this proposal is that methylation status represents a distinct group of CRCs that may independently predict overall survival from CRC. Specifically, the goals are to: 1. Measure the prevalence of hypermethyled genes in sporadic CRC by comparing the methylation panel on available tumor and normal samples (approximately 1450 paired samples) from MECC cases; 2. Determine if patterns of hypermethylation represent distinct subgroups of CRC; 3. Evaluate hypermethylation as an independent prognostic factor in CRC: a. Using Israeli national registries and vital statistics compare survival in subjects; b. Measure survival in the presence of other classically reported prognostic factors. This research addresses the question of whether methylation status defines a subset of tumors with distinct genetic, epigenetic and clinicopathologic characteristics in the general population.