Biophysical studies of a Sulfolobus icosahedral virus

硫化叶菌二十面体病毒的生物物理学研究

• 批准号: 7213323
• 负责人: Reza Khayat
• 金额: $ 5.13万
• 依托单位: SCRIPPS RESEARCH INSTITUTE, THE
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7213323
• 关键词: Adenovirus hexon capsid protein; Adenoviruses; Archaea; Bacteriophage PRD1; Biochemical; Biochemical Genetics; Biochemistry; Biological; Capsid; Capsid Proteins; Circular Dichroism; Complex; Condition; Crenarchaeota; Cryoelectron Microscopy; Data; Depth; Diagnostic radiologic examination; Docking; Electron Microscope; Employee Strikes; Environment; Evolution; Family; Fellowship; Genetic; Imaging Techniques; Individual; Knowledge; Life; Life Cycle Stages; Lytic; Maps; Measures; Melting Point Temperatures; Membrane Lipids; Modeling; Molecular Biology; Names; Organism; Phospholipids; Phycodnaviridae; Phylogenetic Analysis; Phylogeny; Planets; Plant Roots; Proteins; Purpose; Relative (related person); Reporting; Resistance; Resolution; Structure; Sulfolobus; Sulfolobus solfataricus; Techniques; Trees; Viral; Virus; Work; biochemical evolution; concept; hyperthermophile; image reconstruction; insight; intermolecular interaction; member; monolayer; planetary Atmosphere; reconstruction; tool; virology

DESCRIPTION (provided by applicant): The Sulfolobus Turreted Icosahedral virus (STIV) is a lytic hyperthermophile virus that infects members of the Sulfolobus family within the kingdom Crenarchaeota of the domain Archaea. The cryo-electron microscope (Cryo-EM) image reconstruction of the virus shows a striking architectural resemblance to the mammalian adenovirus, the bacteriophage PRD1, and algal virus PBCV-1. Structural studies of the major capsid proteins of adenovirus, PRD1, and PBCV-1 have shown a strong possiblity of evolutionary relationship between these viruses. This proposal sets forth structural and biophysical studies aimed in characterizing STIV and identifying whether this virus can be evolutionary related to the above mentioned viruses. The identification of a virus phylogeny encompassing the three divisions of life is exciting and unprecedented. Characterization of STIV, and Archaea infecting viruses in general, will provide substantial insight into the biochemistry of Archaea, virus host relationship and evolution, and biochemical adaptations required for survival under extreme environments. Detailed characterization of Archaea viruses may also expand our knowledge of viral evolution.

描述（由申请人提供）： Sulfolobus转塔二十面体病毒（STIV）是一种裂解的高疗病毒，可感染域古细菌王国crenarchaeota内的磺胺sullobus家族成员。病毒的冷冻电子显微镜（Cryo-EM）图像重建与哺乳动物腺病毒，噬菌体PRD1和Algal病毒PBCV-1的结构相似。腺病毒，PRD1和PBCV-1的主要衣壳蛋白的结构研究表明，这些病毒之间的进化关系很强。该提案阐述了旨在表征性肺传播的结构和生物物理研究，并确定该病毒是否可以与上述病毒相关。鉴定涵盖生命三个分裂的病毒系统发育是令人兴奋且前所未有的。一般而言，STIV和古细菌感染病毒的表征将为古细菌，病毒宿主关系和进化以及在极端环境中生存所需的生化适应性提供大量见解。古细菌病毒的详细表征也可能扩大我们对病毒进化的了解。