Proton Electron Double Resonance Imaging (PEDRI) of Free Radicals

自由基的质子电子双共振成像 (PEDRI)

• 批准号: 7269814
• 负责人: JAY Louis ZWEIER
• 金额: $ 85.19万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-30 至 2009-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7269814
• 关键词: Algorithms; Anatomy; Animal Disease Models; Animals; Computer software; Data; Data Collection; Deposition; Detection; Development; Electrons; Free Radicals; Heating; Image; Label; Life; Measurement; Nuclear; Numbers; Protons; Resolution; Sampling; Spectrum Analysis; System; Techniques; Technology; Testing; Time; base; design; in vivo; innovation; instrument; instrumentation; novel

DESCRIPTION (provided by applicant): This proposal is for development of novel instrumentation to enable rapid measurement and imaging of free radicals in biomedical applications using Dynamic Nuclear Polarization (DNP) and Proton Electron Double Resonance Imaging (PEDRI). A unique variable field, rapid field cycling (FC) PEDRI instrument will be built capable of performing measurements from fields of 0 to 0.2 T. DNP/PEDRI can provide rapid high quality measurements of free radicals in living animals and these novel dual resonance techniques overcome a number of the fundamental limitations of EPR imaging that requires relatively long times for image data collection, provides limited image resolution due to the inherently broad linewidths of paramagnetic labels, and needs very large gradients as well as independent techniques for anatomic registration. Therefore, DNP and PEDRI have great potential to advance this field providing: sensitive, rapid and high quality measurements and imaging of paramagnetic molecules in biomedical systems. There are 5 aims: 1) Development of a magnet, gradient, field control, and system control interface optimized for PEDRI of free radicals in small animal models of disease; 2) Development of DNP spectroscopy and PEDRI with variable field EPR preexcitation for NMR based detection and imaging of the EPR spectra of free radicals; 3) Development of Field Cycled DNP and Spectral-Spatial PEDRI to enhance measurement sensitivity and minimize problems with power deposition and sample heating; 4) Development of innovative resonator and RF design optimized for DNP and PEDRI of in vivo and ex vivo biomedical applications; 5) Development of optimized software and algorithms enabling rapid acquisition and analysis of DNP and PEDRI spectral and image data. Together these aims will overcome existing limitations of RF power deposition and result in the development of a unique variable field rapid field cycling PEDRI/DNP instrument capable of rapid, high quality measurement and imaging of free radicals in small animal models of disease. This new instrumentation and related innovations in rapid field cycling, resonator technology and ultra fast image acquisition, have the potential to provide 100-fold enhanced sensitivity in radical detection, with 10-fold higher image resolution and over 10-fold faster image acquisition than prior fixed field PEDRI instrumentation, while reducing RF power deposition by over two orders of magnitude.

描述（由申请人提供）：本提案旨在开发新型仪器，以便在生物医学应用中使用动态核极化（DNP）和质子电子双共振成像（PEDRI）快速测量和成像自由基。将构建一种独特的可变场、快速场循环（FC）PEDRI仪器，能够在0至0.2 T的场范围内进行测量。DNP/PEDRI可以提供活体动物中自由基的快速高质量测量，并且这些新颖的双共振技术克服了EPR成像的许多基本限制，EPR成像需要相对长的时间用于图像数据收集，由于顺磁性标记的固有宽线宽而提供有限的图像分辨率，并且需要非常大的梯度以及用于解剖配准的独立技术。因此，DNP和PEDRI在推进该领域方面具有巨大潜力，可提供：生物医学系统中顺磁分子的灵敏、快速和高质量的测量和成像。有五个目标：2）开发DNP光谱和具有可变场EPR预激发的PEDRI，用于自由基的EPR光谱的基于NMR的检测和成像; 3）开发场循环DNP和光谱-空间PEDRI以增强测量灵敏度并最小化功率沉积和样品加热的问题; 4）开发针对体内和体外生物医学应用的DNP和PEDRI优化的创新谐振器和RF设计; 5）开发优化的软件和算法，能够快速采集和分析DNP和PEDRI光谱和图像数据。这些目标将共同克服现有的RF功率沉积的限制，并导致开发一种独特的可变场快速场循环PEDRI/DNP仪器，能够快速，高质量地测量和成像的小动物疾病模型中的自由基。这种新仪器和快速场循环、谐振器技术和超快图像采集方面的相关创新，有可能在自由基检测中提供100倍的增强灵敏度，图像分辨率比现有固定场PEDRI仪器高10倍，图像采集速度比现有固定场PEDRI仪器快10倍以上，同时将RF功率沉积减少两个数量级以上。