Integration of High Field MRI and EPRI For Functional Imaging

高场 MRI 和 EPRI 的集成用于功能成像

• 批准号: 8919363
• 负责人: JAY Louis ZWEIER
• 金额: $ 58.37万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-01 至 2016-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8919363
• 关键词: Address; Algorithmic Software; Anatomy; Animal Disease Models; Animal Model; Animals; Back; Biochemical; Cardiac; Cardiovascular Physiology; Cardiovascular system; Cell Survival; Cell membrane; Cell physiology; Chemicals; Computer software; Consumption; Coupling; Data; Data Collection; Development; Disease; Electrons; Free Radicals; Frequencies; Functional Imaging; Generations; Health; Heart; Heart Diseases; Image; Imaging technology; Injury; Life; Magnetic Resonance; Magnetic Resonance Imaging; Maps; Measurement; Measures; Mediator of activation protein; Metabolism; Modality; Modeling; Motion; Myocardial; Myocardial Infarction; Nitric Oxide; Noise; Organ; Organ Model; Oxidation-Reduction; Oxygen; Oxygen saturation measurement; Pathology; Perfusion; Physiological; Physiology; Power Sources; Protons; Reactive Oxygen Species; Respiration; Role; Sampling; Scanning; Source; Spin Trapping; Stress; System; Techniques; Technology; Testing; Time; Tissues; Work; base; cardiovascular injury; cell injury; free radical oxygen; heart function; image processing; image registration; imaging modality; in vivo; innovation; instrument; instrumentation; prevent; programs; prototype; software development; tool

DESCRIPTION (provided by applicant): Paramagnetic molecules including oxygen, oxygen radicals and nitric oxide modulate cellular function and injury, altering critical cellular processs such as respiration and redox state with changes in tissue perfusion, energetic state and viability. In view of the critical importance of these interactions in normal cardiovascular functin and disease, there has been a great need for an approach to combine in vivo measurement and imaging of paramagnetic molecules with imaging of tissue and organ biochemical and functional parameters in animal models of disease. EPR imaging (EPRI) is a powerful technique that enables 3D spatial in vivo mapping of radical metabolism, oxygenation, and nitric oxide; however, the utility and power of EPRI has been greatly limited by the need for complementary anatomic and functional data. NMR based MRI, in addition to providing anatomic registration, also can provide critical functional information that is needed to interpret the EPRI data including, imaging of tissue perfusion and viability. Integrated EPR/NMR imaging technology and instrumentation has the unique potential to enable in vivo mapping of free radicals, oxygen and nitric oxide along with NMR based functional and anatomic imaging. This technology is very promising but several fundamental problems presently limit its development and biomedical application. These include the need to: [a] provide seamless integration of the EPR and NMR-MRI systems to enable rapid transition between these two imaging modalities; [b] build dual mode resonators optimized for both EPR and NMR resonances suited for specific biomedical applications; [c] accelerate the process of image data collection in both modes facilitating dual mode anatomic and functional imaging; [d] increase sensitivity and minimize motion-induced noise or image distortion; [e] integrate the EPRI and MRI results and analysis in order to perform accurate image co-registration, fast computation and interpretation of functional data. These critical needs will be addressed with a combination of hardware and software development followed by testing in cardiovascular applications as described in 3 specific aims: I. Development and construction of EPRI hardware optimized for high sensitivity fast scan image acquisition and integration of it with high field MRI; II. Development / implementation of integrated software and algorithms for fast EPRI and NMR MRI acquisition with cardiac gating enabling co-mapping of anatomic structure, perfusion, energetic state and viability (MRI) with free radical distribution, redox state and oximetry (EPRI). EPRI acquisition will be accelerated and refined using NMR image information to refine EPR data collection; III. Application for dual mode functional imaging of the heart with both in vivo and ex vivo measurement of tissue O2 levels, redox status, nitric oxide formation (EPRI) in relation to perfusion, viability, and energeic state (MRI). This program will realize the great synergy and power of integrated real time EPRI and high field MRI optimized for biomedical measurement and imaging of free radicals, redox state, O2, and nitric oxide and their role in function and disease.

描述(申请人提供)：包括氧、氧自由基和一氧化氮在内的顺磁性分子调节细胞功能和损伤，随着组织灌流、能量状态和生存能力的变化而改变关键的细胞过程，如呼吸和氧化还原状态。鉴于这些相互作用在正常心血管功能和疾病中的重要作用，迫切需要一种方法，将体内顺磁分子的测量和成像与疾病动物模型中组织和器官的生化和功能参数的成像相结合。EPR成像(EPRI)是一种强大的技术，可以在体内对自由基代谢、氧合和一氧化氮进行3D空间成像；然而，由于需要补充解剖和功能数据，EPRI的用途和能力受到了极大的限制。基于核磁共振的MRI除了提供解剖配准外，还可以提供解释EPRI数据所需的关键功能信息，包括组织灌注和生存能力的成像。集成的EPR/核磁共振成像技术和仪器具有独特的潜力，可以在体内绘制自由基、氧和一氧化氮的图谱，以及基于核磁共振的功能和解剖成像。这项技术非常有前景，但目前有几个根本性的问题限制了它的发展和生物医学应用。这些需求包括：[a]提供EPR和核磁共振-MRI系统的无缝集成，以实现这两种成像模式之间的快速转换；[b)构建针对特定生物医学应用的EPR和核磁共振共振进行优化的双模谐振器；[c)加快两种模式下的图像数据收集进程，以促进双模解剖和功能成像；[d)提高灵敏度并将运动引起的噪声或图像失真降至最低；[e]整合EPRI和MRI结果和分析，以便进行准确的图像共配准、快速计算和功能数据的解释。这些关键需求将通过硬件和软件开发以及随后在心血管应用中的测试相结合来解决，具体目标如下：i.开发和构建针对高灵敏度快速扫描图像采集进行优化的EPRI硬件，并将其与高场MRI集成；ii.开发/实施集成软件和算法，用于快速EPRI和核磁共振成像采集，并通过心脏门控实现解剖结构、灌注、能量状态和生存能力(MRI)与自由基分布、氧化还原状态和血氧仪(EPRI)的共同映射。将利用核磁共振图像信息加速和改进EPRI的获取，以改进EPR数据收集；iii.应用于心脏双模式功能成像，包括体内和体外测量组织O2水平、氧化还原状态、一氧化氮形成(EPRI)与灌注、活力和能量状态(MRI)的关系。该计划将实现集成的实时EPRI和高场MRI的巨大协同和强大功能，这些MRI针对自由基、氧化还原状态、O2和一氧化氮及其在功能和疾病中的作用的生物医学测量和成像进行了优化。