Design and Synthesis of Fluorescent Amino Acids: Novel Tools for Biological Imaging

荧光氨基酸的设计与合成：生物成像的新工具

• 批准号: 2888395
• 金额: --
• 依托单位: University of Glasgow
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2023
• 资助国家: 英国
• 起止时间: 2023 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2888395
• 关键词: Design; Synthesis; Fluorescent; Amino; Acids

This project aims to develop novel fluorescent amino acids with clearly defined properties that can act as imaging probes for molecular and cellular biological applications. Several classes of novel N-heterocycle-derived amino acids will be prepared using metal-catalysed bond forming reactions and cycloaddition processes. Lead compounds from each class of amino acid that show strong fluorescence, high quantum yields and interesting solvatochromatic properties will be further investigated as probes for biological imaging. These will be incorporated into short cell-penetrating peptides using solid phase peptide synthesis, incubated with various cell lines and analysed using confocal fluorescent microscopy.

该项目旨在开发具有明确特性的新型荧光氨基酸，可作为分子和细胞生物学应用的成像探针。几种新型n -杂环衍生氨基酸将采用金属催化成键反应和环加成工艺制备。从每一类氨基酸中提取的具有强荧光、高量子产率和有趣的溶剂化染色性质的先导化合物将被进一步研究作为生物成像探针。利用固相肽合成技术，这些将被纳入短细胞穿透肽中，与各种细胞系一起培养，并使用共聚焦荧光显微镜进行分析。