CORE--NEUROPATHOLOGY

核心--神经病理学

• 批准号: 7210675
• 负责人: LAWRENCE A. HANSEN
• 金额: $ 26.69万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7210675
• 关键词: Alzheimer' s disease; Lewy body; alpha synuclein; amyloid proteins; biomedical facility; brain; brain disorder diagnosis; choline acetyltransferase; cryopreservation; disease /disorder classification; histopathology; human tissue; image processing; immunocytochemistry; neuritic plaques; neuropathology; postmortem; stainings; tissue /cell preparation; tissue resource /registry; ubiquitin

Studies conducted by the Neuropathology Core during the previous period of funding Core in collaboration with other components of the ADRC and national and international researchers have been instrumental in delineating structural correlates of dementia in AD, identifying new neuropathological entities causing dementia, in helping to determine relationships between genotype and neuropathology and better understanding the mechanisms of neurodegeneration in AD. In this context, the main objective of the Neuropathology Core is to provide the projects and other Cores with expert state of the art neuropathological analysis and high quality, well characterized brain tissues from controls, early/moderate severe AD, and DLB cases. In this context the specific aims are: 1) To obtain brains from the Alzheimer Disease Research Center (ADRC) participants within 8 hrs after death, using a standardized protocol. 2) To provide neuropathological diagnoses of demented and normal aged (control) patients clinically evaluated by the San Diego ADRC, using standardized methods. 3) To perform lesion counts and Braak staging for AD and Dementia with Lewy bodies Disease (DLB). 4) To perform neurochemical analysis relevant to neurodegeneration and amyloid production in frozen brain samples. 5) To maintain a state of the art Alzheimer's brain bank with well characterized cases including Mild Cognitive Impairment (MCI) cases. This resource will continue to provide high quality fresh, frozen and fixed brain tissues to ADRC researchers as well as to national and international collaborators. Special emphasis will be placed at obtaining cases with MCI. The neuropathological and neurochemical data collected in these cases will be used for subsequent clinico-pathological correlations. A web-based database and web site is maintained for tissue requests, tissue logs and disbursements. The Core also provides data and tissues to other components of the ADRC and to national and international collaborators.

神经病理学核心在上一期资助核心的研究中进行的研究 与ADRC的其他组成部分以及国家和国际研究人员的合作有助于描述AD中痴呆症的结构相关性，确定引起痴呆症的新神经病理学实体，以帮助确定基因型与神经病理学之间的关系，并更好地理解AD中神经变性的机制。在这种情况下，神经病理学核心的主要目标是为项目和其他核心提供最先进的神经病理学分析和高质量，高质量的，具有对照，早期/中度严重AD和DLB病例的脑组织的高质量，表征良好的脑组织。在这种情况下，具体目的是：1）使用标准化方案，在死后8小时内从阿尔茨海默氏病研究中心（ADRC）参与者获取大脑。 2）使用标准化方法，通过圣地亚哥ADRC评估了痴呆症和正常老年（对照）患者的神经病理学诊断。 3）对Lewy Body疾病（DLB）进行AD和痴呆症的病变计数和BRAAK分期。 4）在冷冻脑样品中进行与神经变性和淀粉样蛋白产生有关的神经化学分析。 5）维持最先进的阿尔茨海默氏症脑库的状态 轻度认知障碍（MCI）病例。该资源将继续为ADRC研究人员以及国家和国际合作者提供高质量的新鲜，冷冻和固定的脑组织。将特别重点放在获得MCI病例的情况下。在这些情况下收集的神经病理学和神经化学数据将用于随后的临床病理相关性。维护基于Web的数据库和网站，以进行组织请求，组织日志和支出。核心还为ADRC的其他组件以及国家和国际合作者提供数据和组织。