Acoustic detection of viruses bacteria and toxins

描述(由调查人员提供)： 阿库比奥与USAMRIID(德特里克堡)合作，打算1.确定声学检测是否可以区分对人类健康构成威胁的非致病和“优先”病原体。将对声学探测用于快速识别和量化从NIAID A、B或C类清单中选择的病毒、细菌和毒素进行初步调查。这项工作将为生物防御、病原体和毒素检测领域的现有检测技术建立性能基线。2.将该材料与特定的寡核苷酸探针杂交，应用声学检测直接检测致病核酸。其目的是开发一种直接的、无标记的方法来同时检测致病粒子、抗原和核酸，从而大大减少检测时间。3.开发多路复用仪器和消耗品，以允许同时对多个试剂进行测试。这将通过结合使用一系列声学传感器、适当的抗体和针对一系列A、B或C类病原体的核酸探针来实现。对于特定的病原体，将获得与阵列上适当受体的抗原结合和核苷酸结合的概况。4.评估并确定生产小型便携式设备的可行性。该样机的性能将与实验室仪器进行严格的比较。将为该手持单元的商业开发和制造制定产品规格和项目计划。

DESCRIPTION (provided by investigators): Akubio, in collaboration with USAMRIID (Fort Detrick) intend to 1. Determine whether acoustic detection can differentiate between non-pathogenic and "priority" pathogenic organisms that are a threat to human health. An initial investigation into the use of acoustic detection for the rapid identification and quantization of a virus, a bacterium and a toxin, selected from the NIAID Category A, B or C list will be carried out. This work will establish performance baselines for the existing detection technology in the area of biodefense pathogen and toxin detection. 2. Apply acoustic detection to direct detection of pathogenic nucleic acid via hybridization of this material to specific oligonucleotide probes. The aim is to develop a direct, label-free method to detect pathogenic particles, antigens and nucleic acids simultaneously leading to a substantial reduction in assay time. 3. Develop multiplexed instrumentation and consumables to permit simultaneous testing for multiple agents. This will be achieved with an array of acoustic sensors used together, appropriate antibodies, and nucleic acid probes against a range of Category A, B or C pathogens. Profiles for antigen binding and nucleotide binding to appropriate receptors on the array will be obtained for specific pathogens. 4. Assess and establish the feasibility for production of a small portable device. The performance of this prototype will be compared rigorously with the laboratory-based instrumentation. A product specification and project plan for commercial development and manufacture of this hand-held unit will be produced.