Acoustic detection of viruses bacteria and toxins

病毒、细菌和毒素的声学检测

• 批准号: 6818239
• 负责人: Matthew A Cooper
• 金额: $ 82.75万
• 依托单位: AKUBIO, LTD
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-03-01 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6818239
• 关键词: British Isles; United States; auditory discrimination; bacteria; biohazard detection; biomedical equipment development; bioterrorism /chemical warfare; communicable disease diagnosis; cooperative study; diagnosis design /evaluation; miniature biomedical equipment; nucleic acid hybridization; nucleic acid probes; oligonucleotides; portable biomedical equipment; rapid diagnosis; receptor binding; toxin; virus

DESCRIPTION (provided by investigators): Akubio, in collaboration with USAMRIID (Fort Detrick) intend to 1. Determine whether acoustic detection can differentiate between non-pathogenic and "priority" pathogenic organisms that are a threat to human health. An initial investigation into the use of acoustic detection for the rapid identification and quantization of a virus, a bacterium and a toxin, selected from the NIAID Category A, B or C list will be carried out. This work will establish performance baselines for the existing detection technology in the area of biodefense pathogen and toxin detection. 2. Apply acoustic detection to direct detection of pathogenic nucleic acid via hybridization of this material to specific oligonucleotide probes. The aim is to develop a direct, label-free method to detect pathogenic particles, antigens and nucleic acids simultaneously leading to a substantial reduction in assay time. 3. Develop multiplexed instrumentation and consumables to permit simultaneous testing for multiple agents. This will be achieved with an array of acoustic sensors used together, appropriate antibodies, and nucleic acid probes against a range of Category A, B or C pathogens. Profiles for antigen binding and nucleotide binding to appropriate receptors on the array will be obtained for specific pathogens. 4. Assess and establish the feasibility for production of a small portable device. The performance of this prototype will be compared rigorously with the laboratory-based instrumentation. A product specification and project plan for commercial development and manufacture of this hand-held unit will be produced.

描述（调查人员提供）： Akubio与Usamriid（Fort Detrick）合作打算1。确定声学检测是否可以区分对人类健康构成威胁的非致病性和“优先”病原体。将对从NIAID A，B类或C列表中选择的病毒，细菌和A毒素快速鉴定和量化的声学检测的初步研究。这项工作将在生物探测病原体和毒素检测领域建立现有检测技术的性能基准。 2。通过将这种材料杂交与特定的寡核苷酸探针杂交直接检测声学检测来直接检测致病性核酸。目的是开发一种无标记的方法，以同时检测病原颗粒，抗原和核酸，从而导致测定时间大幅减少。 3。开发多路复用的仪器和消耗品，以允许对多种代理进行同时测试。这将通过一起使用的一系列声传感器，适当的抗体和针对A类A，B类或C病原体的核酸探针来实现。对于特定病原体，将获得抗原结合和核苷酸结合与阵列上适当受体的轮廓。 4。评估并确定生产小型便携式设备的可行性。该原型的性能将与基于实验室的仪器进行严格的比较。将制定用于商业开发和制造此手持单元的产品规范和项目计划。