Quantitative CT-Based Lung Atlas of the Mouse

描述（由申请人提供）：这是一项生物工程研究资助申请，旨在建立用于以可重复方式对小鼠肺部进行成像的微型CT方法，以便图像数据适用于定量局部肺结构（包括实质密度）。将建立识别肺、肺叶、气道和肺泡结构的方法。我们将使用这些方法建立C57 BI/6 J小鼠的图谱数据库/知识库，并建立A/J小鼠的另一个数据库，因为这些品系之间的解剖变异性。本项目完成后，我们将为C57 BI/6 J和A/J小鼠染色生成规范的解剖参考数据库，其中包括10只小鼠的图像数据和总计100只小鼠的几何测量值。所有100只小鼠的图像数据将离线存储，但出于对在线“实时”数据存储考虑的实际限制，我们将在线保存的图像数据限制为每种品系10个。每只小鼠将至少有3个体积图像数据集、分割结果、用于CFD和其他建模研究的网格数据集、纹理结果等。在本项目中获得的知识将使我们能够为构建其他菌株和场景的未来数据库提供建议。此外，我们强调，我们不仅将公开提供地图集，我们还欢迎其他团体提供与我们不提供的数据集相关的测量和分类（或改进或在某种程度上不同于我们提供的测量）。图谱数据库将通过从新鲜死亡小鼠的原位肺的高分辨率MicroCT扫描和3D病理图像构建模型来构建。用于构建每个图谱数据库的图像数据将包括3个肺体积（5、10和25 cm H2O）的MicroCT图像和一个肺体积（25 cm H2O）的病理图像。通过与Ochs博士和Weibel博士的合作，我们还将建立显微CT和显微镜方法之间的相关性。显微镜检查还将用于为小鼠图谱提供补充数据。将开发通过Micro CT跟踪活体小鼠的方法，以跟踪病理过程，从而通过比较个体和标准图谱，帮助决定适当的时间点进行更详细的尸检和原位分析。

DESCRIPTION (provided by applicant): This is a request for a bioengineering research grant to establish micro CT methods for imaging mouse lungs in a repeatable manner such that image data is suitable for quantitating regional lung structure including parenchymal density. Methods for identifying the lung, lung lobes, airways and alveolar structure will be established. We will use these methods to then establish an atlas database/knowledgebase for C57BI/6J mice and another database for A/J mice due to the anatomical variability across these strains. Upon completion of this project we will have produced normative anatomical reference databases for the C57BI/6J and A/J mice stains consisting of image data from 10 mice each and geometrical measurements from a total of 100 mice each. Image data from all 100 mice will be stored off line, but we limit the image data to be held on line to 10 from each strain out of consideration for practical limitations to on-line "live" data storage considerations. Each mouse will have at least 3 volumetric image data sets, segmentation results, meshed datasets for use in CFD and other modeling studies, texture results, etc. the knowledge gained in this project will allow us to provide recommendations for constructing future databases for other strains and scenarios. Furthermore, we emphasize that we will not only make the atlas available publicly, we will welcome other groups to contribute measurements and categorizations associated with the data set which we do not provide (or which improve upon or differ in some way from those measures which we provide). The atlas databases will be constructed by building models from high resolution MicroCT scans and 3D pathological images of in situ lungs from freshly dead mice. Image data used to construct each atlas database will consist of MicroCT images at 3 lung volumes (5, 10 and 25 cmH2O) and pathological images at one lung volume (25 cmH2O). Through a collaboration with Drs Ochs and Weibel, we will also establish the correlations between micro-CT and microscopy-based methods. Microscopy will be used to also provide supplemental data to the mouse atlas. Methods will be developed to follow living mice via Micro CT to allow for the tracking of pathologic processes to aid in the decision as to appropriate time points for more detailed post mortem, in situ analyses through comparisons between the individual and the normative atlas.