Quantitative CT-Based Lung Atlas of the Mouse

描述（由申请人提供）：这是一份生物工程研究拨款申请，旨在建立微型CT方法，以可重复的方式对小鼠肺部进行成像，使图像数据适合于定量区域肺结构，包括实质密度。建立肺、肺叶、气道和肺泡结构的鉴别方法。我们将利用这些方法建立C57BI/6J小鼠的图谱数据库/知识库和A/J小鼠的数据库，因为这些菌株之间存在解剖差异。本项目完成后，我们将为C57BI/6J和A/J小鼠染色建立规范的解剖学参考数据库，包括每10只小鼠的图像数据和每100只小鼠的几何测量数据。所有100只小鼠的图像数据将离线存储，但出于对在线“实时”数据存储考虑的实际限制，我们将每个品系的在线图像数据限制为10个。每只小鼠将至少有3个体积图像数据集，分割结果，用于CFD和其他建模研究的网格数据集，纹理结果等。在本项目中获得的知识将使我们能够为构建其他菌株和场景的未来数据库提供建议。此外，我们强调，我们不仅将公开提供地图集，我们还将欢迎其他团体贡献与我们没有提供的数据集相关的测量和分类（或在某些方面改进或不同于我们提供的测量）。图谱数据库将通过高分辨率MicroCT扫描和新鲜死亡小鼠原位肺的3D病理图像建立模型来构建。用于构建每个图谱数据库的图像数据将包括3个肺体积（5、10和25 cmH2O）的MicroCT图像和一个肺体积（25 cmH2O）的病理图像。通过与Ochs博士和Weibel博士的合作，我们还将建立micro-CT和基于显微镜的方法之间的相关性。显微镜还将用于为小鼠图谱提供补充数据。将开发通过Micro CT跟踪活小鼠的方法，以允许跟踪病理过程，以帮助决定适当的时间点，以便通过个体和规范图谱之间的比较进行更详细的死后原位分析。

DESCRIPTION (provided by applicant): This is a request for a bioengineering research grant to establish micro CT methods for imaging mouse lungs in a repeatable manner such that image data is suitable for quantitating regional lung structure including parenchymal density. Methods for identifying the lung, lung lobes, airways and alveolar structure will be established. We will use these methods to then establish an atlas database/knowledgebase for C57BI/6J mice and another database for A/J mice due to the anatomical variability across these strains. Upon completion of this project we will have produced normative anatomical reference databases for the C57BI/6J and A/J mice stains consisting of image data from 10 mice each and geometrical measurements from a total of 100 mice each. Image data from all 100 mice will be stored off line, but we limit the image data to be held on line to 10 from each strain out of consideration for practical limitations to on-line "live" data storage considerations. Each mouse will have at least 3 volumetric image data sets, segmentation results, meshed datasets for use in CFD and other modeling studies, texture results, etc. the knowledge gained in this project will allow us to provide recommendations for constructing future databases for other strains and scenarios. Furthermore, we emphasize that we will not only make the atlas available publicly, we will welcome other groups to contribute measurements and categorizations associated with the data set which we do not provide (or which improve upon or differ in some way from those measures which we provide). The atlas databases will be constructed by building models from high resolution MicroCT scans and 3D pathological images of in situ lungs from freshly dead mice. Image data used to construct each atlas database will consist of MicroCT images at 3 lung volumes (5, 10 and 25 cmH2O) and pathological images at one lung volume (25 cmH2O). Through a collaboration with Drs Ochs and Weibel, we will also establish the correlations between micro-CT and microscopy-based methods. Microscopy will be used to also provide supplemental data to the mouse atlas. Methods will be developed to follow living mice via Micro CT to allow for the tracking of pathologic processes to aid in the decision as to appropriate time points for more detailed post mortem, in situ analyses through comparisons between the individual and the normative atlas.