Microcomputed tomography device (Scanco microCT35)

微型计算机断层扫描设备（Scanco microCT35）

• 批准号: 7389328
• 负责人: KARL Leonard INSOGNA
• 金额: $ 23.33万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-25 至 2008-09-24
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7389328
• 关键词: Academic Medical Centers; Animal Model; Animals; Arts; Award; Biology; Boston; Commit; Connecticut; Development; Developmental Biology; Devices; Discipline of obstetrics; Epiphysial cartilage; Evaluation; Familial hypophosphatemic bone disease; Fibroblast Growth Factor; Finite Element Analysis; Funding; Gynecology; Image; Imaging Device; Immunobiology; Institution; Medicine; Methodology; Methods; Mission; Mus; Musculoskeletal; Musculoskeletal Diseases; Orthopedic Surgery procedures; Outsourcing; Pathology; Pediatric Surgical Procedures; Physiology; Pilot Projects; Research; Research Personnel; Resolution; Role; Running; Sampling; Science; Scientist; Signal Transduction; Skeletal system; TNFRSF5 gene; Technology; Tissues; Translations; Universities; bone; inorganic phosphate; member; programs; ranpirnase; research study; substantia spongiosa; tomography

DESCRIPTION (provided by applicant): This proposal seeks funding to purchase a high-resolution desktop microtomographic imaging device (5CT35, Scanco USA, Southeastern, PA). There is an urgent and ongoing need for the ability to do fine structural analysis of hard tissues at Yale University. In 1999, the Yale Core Center for Musculoskeletal Disorders (YCCMD) was established through a P30 Core Center award from NIAMS. This Center was competitively renewed in 2004. The Center has as its mission the development and evaluation of animal models of musculoskeletal disorders. It is currently comprised of 43 members from Departments across the Medical Center and the University, including Medicine, Pediatrics, Surgery, Orthopaedics, Obstetrics & Gynecology, Pathology, Immunobiology, and Developmental Biology. Among these investigators are some of the most prominent scientists in the field of skeletal biology, many of whom have ongoing animal studies as part of their research programs. The YCCMD supports a Pilot Project program, which funds 2 new investigators each year to develop animal models for musculoskeletal disorders. In 2005, Yale was awarded a P50 Center Research Translation, the Yale Center for X-Linked Hypophosphatemic Rickets Research (YC- XLH). This translational award funds 2 large projects involving animal models: one to explore the role of FGF signaling in bone, and one to explore the role of phosphate in regulating the growth plate. In addition, the YC-XLH supports 3 Pilot projects annually that will begin in 2008. It is likely that these Pilot projects will include animal models of musculoskeletal disorders. Currently, the only method available at Yale to estimate bone mass in mice is PIXimus, operated by the Physiology Core of the YCCMD. There is no microCT machine at Yale University. Consequently, investigators requiring this technology have had to outsource their experiments, either to the University of Connecticut microCT facility run by Dr. Douglas Adams, or to Dr. Mary Bouxsein's facility in Boston. Since these machines are already heavily committed, samples from Yale receive lower priority at the collaborating institutions. The YCCMD's Histomorphometry Unit is being overwhelmed with samples in lieu of another methodology to separately determine cortical and trabecular bone mass or to provide detailed microarchitectural analysis of bone. The acquisition of the Scanco 5CT35 will give investigators at Yale state-of-the-art, high-resolution (3.5 micron) imaging capacity, as well as the ability to undertake finite element analysis. These capabilities are absolutely required to sustain the current pace of discovery in musculoskeletal science at Yale.

描述（由申请人提供）：本提案寻求资金购买高分辨率桌面显微断层成像设备（5CT35，Scanco USA，东南，宾夕法尼亚州）。耶鲁大学迫切且持续地需要具备对硬组织进行精细结构分析的能力。 1999 年，耶鲁大学肌肉骨骼疾病核心中心 (YCCMD) 通过 NIAMS 的 P30 核心中心奖成立。该中心于 2004 年进行了竞争性更新。该中心的使命是开发和评估肌肉骨骼疾病的动物模型。该委员会目前由来自医学中心和大学各个部门的 43 名成员组成，包括医学、儿科、外科、骨科、妇产科、病理学、免疫生物学和发育生物学。 Among these investigators are some of the most prominent scientists in the field of skeletal biology, many of whom have ongoing animal studies as part of their research programs. YCCMD 支持一项试点项目计划，该计划每年资助 2 名新研究人员开发肌肉骨骼疾病的动物模型。 2005年，耶鲁大学被授予P50中心研究成果转化奖，即耶鲁大学X连锁低磷血症性佝偻病研究中心（YC-XLH）。该转化奖资助了两个涉及动物模型的大型项目：一个是探索 FGF 信号在骨骼中的作用，另一个是探索磷酸盐在调节生长板中的作用。此外，YC-XLH 每年支持 3 个试点项目，将于 2008 年开始。这些试点项目很可能包括肌肉骨骼疾病的动物模型。目前，耶鲁大学唯一可用的估算小鼠骨量的方法是 PIXimus，由 YCCMD 生理学核心操作。耶鲁大学没有 microCT 机。因此，需要这项技术的研究人员不得不将他们的实验外包给 Douglas Adams 博士运营的康涅狄格大学 microCT 设施，或者外包给 Mary Bouxsein 博士在波士顿的设施。由于这些机器已经大量投入使用，因此耶鲁大学的样本在合作机构中的优先级较低。 YCCMD 的组织形态计量学单位正被大量样本所淹没，无法替代另一种方法来分别确定皮质骨和小梁骨质量或提供详细的骨微结构分析。收购 Scanco 5CT35 将为耶鲁大学的研究人员提供最先进的高分辨率（3.5 微米）成像能力以及进行有限元分析的能力。这些能力对于维持耶鲁大学目前肌肉骨骼科学的发现速度是绝对必要的。