Vevo 770 High Resolution Ultrasound Imaging System

Vevo 770 高分辨率超声成像系统

• 批准号: 7217076
• 负责人: MARIELLE SCHERRER-CROSBIE
• 金额: $ 28.05万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-06-01 至 2008-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7217076
• 关键词: Aneurysm; Animals; Aorta; Atherosclerosis; Blood Vessels; Cardiac; Cardiovascular Diseases; Cytoskeletal Proteins; Development; Echocardiography; Evaluation; Funding; Genes; Genetically Modified Animals; Heart; Heart failure; High Blood Pressure; Human; Image; Inflammation; Left; Left Ventricular Mass; Left Ventricular Remodeling; Lung; Modeling; Mus; Mutation; Myocardial Infarction; Neonatal; Nitric Oxide Synthase; Numbers; Pathway interactions; Phenotype; Physiology; Process; Pulmonary Hypertension; Rattus; Request for Proposals; Research; Resolution; Right ventricular structure; Rodent; Role; Stem cells; Structure; System; Techniques; Ultrasonography; Ventricular; Viral Antibodies; bone morphogenetic protein receptor type II; follow-up; human NOS3 protein; inhaled nitric oxide; insight; lung injury; novel; pressure; size

DESCRIPTION (provided by applicant): This proposal requests funds to aid in the acquisition the Vevo 770 High Resolution Imaging System, an ultrasound machine dedicated to characterizing mouse and rat models of human cardiovascular disorders. In recent years, the development of genetically-altered mice has enhanced our understanding of the basic mechanisms underlying cardiac physiology and pathological states. Echocardiography has emerged as a crucial noninvasive technique to assess the left ventricular (LV) size and function of small animals, reducing the number of animals necessary to define a cardiac phenotype and allowing for serial evaluation in the same animal. The Vevo 770 will be a dedicated research echocardiogram, easily available for ongoing studies, and will be kept inside the viral-antibody free (barrier) rodent facility, allowing for serial evaluation of genetically-modified animals. The high spatial resolution of the Vevo 770 permits serial evaluation of structures which are not visualized by conventional echocardiograms, such as the right ventricle, neonatal hearts, great vessels. The right ventricular size and function will be followed serially in models of lung injury to assess the efficacy of novel therapies including inhaled nitric oxide (NO) (Dr. Zapol) and in models of pulmonary hypertension associated with mutations in the gene encoding BMPR-II (Dr. Bloch). Left ventricular mass and function will be assessed precisely and repeatedly in models of pressure-overload to gain insights into the role of nitric oxide synthase 3 (Dr. Bloch) and cytoskeletal proteins (Dr. Chien). Assessment of rodent LV remodeling after myocardial infarction will allow better understanding of the mechanisms involved in this process (Dr. Levine). Serial follow-up of the aorta and carotids will help to determine the impact of NO synthases on atherosclerosis (Dr. Huang) and will provide insights into the role of inflammation in vascular aneurysms formation (Dr. Gerszten). Finally, detecting small quantities of echocardiographic contrast may enable new ways to image stem cells (Dr. Hajjar). Relevance. The Vevo770 is an ultrasound machine dedicated to small animals and capable of noninvasively imaging very fine cardiac or vascular structures. We will use this machine to better understand the pathways involved in pulmonary and systemic high blood pressure, atherosclerosis, heart failure, as well as to attempt to image stem cells.

描述（由申请人提供）：该提案要求提供资金，以帮助收购Vevo 770高分辨率成像系统，这是一种专用于表征人类心血管疾病小鼠和大鼠模型的超声机器。近年来，转基因小鼠的发展增强了我们对心脏生理和病理状态的基本机制的理解。超声心动图已成为一种重要的非侵入性技术，以评估左心室（LV）的大小和功能的小动物，减少动物的数量，必要的定义心脏表型，并允许在同一动物的系列评价。Vevo 770将是一种专用的研究超声心动图，易于用于正在进行的研究，并将保存在无病毒抗体（屏障）啮齿动物设施内，以便对转基因动物进行系列评价。Vevo 770的高空间分辨率允许对常规超声心动图无法显示的结构进行系列评价，例如右心室、新生儿心脏、大血管。将在肺损伤模型中连续跟踪右心室大小和功能，以评估包括吸入一氧化氮（NO）（Zapol博士）在内的新型疗法的疗效，并在与BMPR-II编码基因突变相关的肺动脉高压模型中进行跟踪（Bloch博士）。将在压力超负荷模型中精确和重复评估左心室质量和功能，以深入了解一氧化氮合酶3（Bloch博士）和细胞骨架蛋白（Chien博士）的作用。心肌梗死后啮齿动物LV重塑的评估将有助于更好地理解这一过程中涉及的机制（Levine博士）。对主动脉和颈动脉的连续随访将有助于确定NO代谢酶对动脉粥样硬化的影响（黄博士），并将为炎症在血管动脉瘤形成中的作用提供见解（Gerszten博士）。最后，检测少量的超声心动图对比度可能会使干细胞成像的新方法成为可能（Hajjar博士）。本案无关Vevo 770是一种专用于小动物的超声机器，能够对非常精细的心脏或血管结构进行无创成像。我们将使用这台机器来更好地了解涉及肺和全身高血压，动脉粥样硬化，心力衰竭的途径，以及试图成像干细胞。