Zeiss LSM 510 Laser Scanning Microscope

蔡司 LSM 510 激光扫描显微镜

• 批准号: 7217125
• 负责人: Mercedes Gonzalez-Juarrero
• 金额: $ 43.74万
• 依托单位: COLORADO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7217125
• 关键词: 6 year old; Acquired Immunodeficiency Syndrome; Animal Diseases; Animals; Bacteria; Biotechnology; Cells; Cellular Structures; Chromosomes; Colorado; Communicable Diseases; Confocal Microscopy; Contract Services; Data; Dengue; Detection; Diagnosis; Disease; Enzymes; Equine Encephalomyelitis; Equipment; Funding; Generations; Human; Image; Immunology; Infection; Insecta; Laboratories; Laser Scanning Confocal Microscopy; Lasers; Membrane; Methods; Microbiology; Microscope; Molecular; Parasites; Pathogenesis; Pathology; Physiological; Plague; Principal Investigator; Productivity; Reproduction; Research; Research Personnel; Running; Scanning; Schistosomiasis; Scientist; System; Technology; Tissues; Tuberculosis; United States National Institutes of Health; Universities; Virus; West Nile virus; cost; design; drug development; instrument; microbial; microorganism immunology; vaccine development

DESCRIPTION (provided by applicant): Laser Confocal Microscopy combined with the latest technology for collecting data imaging allows for the precise, scientific analysis and characterization of even the rarest and most difficult to study cellular structures. Thus, confocal microscopy is capable of imaging tissue or single cells from human, animals or insects but also bacteria, chromosomes, and even enzymes and molecules. Confocal microscopy is essential to the NIH funded research of at least fourteen scientists at the department of Microbiology, Immunology & Pathology (MIP) of Colorado State University (CSU). These projects include pathogenesis of microorganisms, immunology and vaccine development, drug development, and new methods of diagnosis and detection of human or animal infectious diseases. Within this frame of research, scientists of the MIP department covers a broad spectrum of human and animal disease including tuberculosis, lepra, Dengue, AIDS, West Nile and other equine encephalitides, Schistosomiasis and Plague. Lack of a confocal microscope designed with a fast scanning and parallel acquisition system is severely hampering productivity and generation of high quality subcellular images of microbial (bacteria, viruses, parasites) infection of single cells or tissue and correlation of physiological and structural changes due to alterations in molecular composition of membranes or subcellular compartments in steady or disease state. Therefore, principal investigators of the MIP with NIH-funded projects are requesting funds to purchase a Zeiss LSM 510 confocal laser Scanning Microscope. The MIP department is spread out between two CSU campuses (the main campus and the Foothills Research Campus) both in Fort Collins, CO but each one separated by a distance of approximately seven miles. The MIP has run successfully a confocal microscopy core unit consisting of a Flowview 300 confocal microscopy since 1999. This instrument is located in the Pathology building located in the main campus of CSU. However, this equipment is already 6 years old, is outdated, designed with a very slow scanning system and far away from the Foothills Research Campus. During the last five years, MIP researchers have surpassed the capabilities of this microscope. The Zeiss LSM 510 Confocal Laser Scanning Microscopy instrument will be located in the Foothills Research Campus and it will belong to the MIP Confocal Microscopy core unit. It will be available to all MIP researchers and to other researchers from other laboratories at the Foothills Research Campus such as ARBL (Animal Reproduction and Biotechnology Laboratories). The MIP department will provide the required funds and space to establish this instrument at the Foothills Research Campus. The initial service contracts will derive from the guarantee of the machine and thereafter, users' feel will be responsible for the cost of the facility.

描述（由申请人提供）：激光共聚焦显微镜与最新的数据成像收集技术相结合，可以对即使是最稀有和最难以研究的细胞结构进行精确，科学的分析和表征。因此，共聚焦显微镜能够对来自人类、动物或昆虫的组织或单细胞进行成像，还能够对细菌、染色体、甚至酶和分子进行成像。共聚焦显微镜是必不可少的国家卫生研究院资助的研究，至少有十四名科学家在微生物学，免疫学和病理学系（MIP）的科罗拉多州立大学（CSU）。这些项目包括微生物的发病机理、免疫学和疫苗开发、药物开发以及诊断和检测人类或动物传染病的新方法。在这一研究框架内，MIP部门的科学家涵盖了广泛的人类和动物疾病，包括结核病，麻风病，登革热，艾滋病，西尼罗河和其他马脑炎，血吸虫病和鼠疫。缺乏设计有快速扫描和并行采集系统的共聚焦显微镜严重阻碍了生产率和单细胞或组织的微生物（细菌、病毒、寄生虫）感染的高质量亚细胞图像的产生，以及由于稳定或疾病状态下膜或亚细胞区室的分子组成的改变而引起的生理和结构变化的相关性。因此，MIP的主要研究者与NIH资助的项目正在申请资金购买蔡司LSM 510共聚焦激光扫描显微镜。MIP部门分布在科罗拉多州柯林斯堡的两个CSU校区（主校区和山麓研究校区）之间，但每个校区之间的距离约为七英里。MIP自1999年以来成功运行了由Flowview 300共聚焦显微镜组成的共聚焦显微镜核心单元。该仪器位于CSU主校区的病理学大楼内。然而，这台设备已经有6年的历史了，已经过时了，设计的扫描系统非常慢，而且离山麓研究园区很远。在过去的五年里，MIP研究人员已经超越了这种显微镜的能力。蔡司LSM 510共聚焦激光扫描显微镜仪器将位于山麓研究园区，它将属于MIP共聚焦显微镜核心单元。它将提供给所有MIP研究人员和其他研究人员从其他实验室在山麓研究校园，如ARBL（动物繁殖和生物技术实验室）。MIP部门将提供所需的资金和空间，在山麓研究校园建立这个工具。最初的服务合同将来自机器的保证，此后，用户将对设施的费用负责。

项目成果

Mycobacterium leprae Infection in a Wild Nine-Banded Armadillo, Nuevo León, Mexico.

• DOI: 10.3201/eid2803.211295
• 发表时间: 2022-03
• 期刊: Emerging infectious diseases
• 影响因子: 11.8
• 作者: Vera-Cabrera L;Ramos-Cavazos CJ;Youssef NA;Pearce CM;Molina-Torres CA;Avalos-Ramirez R;Gagneux S;Ocampo-Candiani J;Gonzalez-Juarrero M;Mayorga-Rodriguez JA;Mayorga-Garibaldi L;Spencer JS;Jackson M;Avanzi C
• 通讯作者: Avanzi C