Zeiss LSM 510 Laser Scanning Microscope

描述（由申请人提供）：激光共聚焦显微镜结合最新的收集数据成像技术，可以对最稀有和最困难的细胞结构进行精确、科学的分析和表征。因此，共聚焦显微镜能够成像组织或单细胞从人，动物或昆虫，但也细菌，染色体，甚至酶和分子。共聚焦显微镜对美国国立卫生研究院资助的科罗拉多州立大学微生物学、免疫学和病理学（MIP）至少14名科学家的研究至关重要。这些项目包括微生物的发病机制、免疫学和疫苗开发、药物开发以及诊断和检测人类或动物传染病的新方法。在这一研究框架内，MIP部门的科学家涵盖了广泛的人类和动物疾病，包括结核病、麻风病、登革热、艾滋病、西尼罗河和其他马脑病、血吸虫病和鼠疫。缺乏设计具有快速扫描和并行采集系统的共聚焦显微镜严重阻碍了单细胞或组织感染微生物（细菌、病毒、寄生虫）的生产力和高质量亚细胞图像的产生，以及由于膜或亚细胞区室在稳定或疾病状态下分子组成的改变而引起的生理和结构变化的相关性。因此，美国国立卫生研究院资助项目的MIP的主要研究者正在申请资金购买一台蔡司LSM 510共聚焦激光扫描显微镜。MIP系分布在科罗拉多州立大学的两个校区（主校区和山麓研究校区）之间，这两个校区都位于科罗拉多州的柯林斯堡，但每一个校区之间相隔大约7英里。自1999年以来，MIP已经成功运行了一个共聚焦显微镜核心单元，包括Flowview 300共聚焦显微镜。该仪器位于科罗拉多州立大学主校区的病理学楼。然而，这个设备已经有6年的历史了，已经过时了，设计的扫描系统非常慢，而且离山麓研究园区很远。在过去的五年里，MIP的研究人员已经超越了这台显微镜的能力。蔡司LSM 510共聚焦激光扫描显微镜仪器将位于山麓研究园区，它将属于MIP共聚焦显微镜核心单元。它将提供给所有MIP研究人员以及来自山麓研究园区其他实验室的其他研究人员，如ARBL（动物生殖和生物技术实验室）。MIP部门将提供所需的资金和空间，以建立该仪器在山麓研究校园。最初的服务合同将从机器的保修开始，此后，用户感觉将负责设备的成本。

DESCRIPTION (provided by applicant): Laser Confocal Microscopy combined with the latest technology for collecting data imaging allows for the precise, scientific analysis and characterization of even the rarest and most difficult to study cellular structures. Thus, confocal microscopy is capable of imaging tissue or single cells from human, animals or insects but also bacteria, chromosomes, and even enzymes and molecules. Confocal microscopy is essential to the NIH funded research of at least fourteen scientists at the department of Microbiology, Immunology & Pathology (MIP) of Colorado State University (CSU). These projects include pathogenesis of microorganisms, immunology and vaccine development, drug development, and new methods of diagnosis and detection of human or animal infectious diseases. Within this frame of research, scientists of the MIP department covers a broad spectrum of human and animal disease including tuberculosis, lepra, Dengue, AIDS, West Nile and other equine encephalitides, Schistosomiasis and Plague. Lack of a confocal microscope designed with a fast scanning and parallel acquisition system is severely hampering productivity and generation of high quality subcellular images of microbial (bacteria, viruses, parasites) infection of single cells or tissue and correlation of physiological and structural changes due to alterations in molecular composition of membranes or subcellular compartments in steady or disease state. Therefore, principal investigators of the MIP with NIH-funded projects are requesting funds to purchase a Zeiss LSM 510 confocal laser Scanning Microscope. The MIP department is spread out between two CSU campuses (the main campus and the Foothills Research Campus) both in Fort Collins, CO but each one separated by a distance of approximately seven miles. The MIP has run successfully a confocal microscopy core unit consisting of a Flowview 300 confocal microscopy since 1999. This instrument is located in the Pathology building located in the main campus of CSU. However, this equipment is already 6 years old, is outdated, designed with a very slow scanning system and far away from the Foothills Research Campus. During the last five years, MIP researchers have surpassed the capabilities of this microscope. The Zeiss LSM 510 Confocal Laser Scanning Microscopy instrument will be located in the Foothills Research Campus and it will belong to the MIP Confocal Microscopy core unit. It will be available to all MIP researchers and to other researchers from other laboratories at the Foothills Research Campus such as ARBL (Animal Reproduction and Biotechnology Laboratories). The MIP department will provide the required funds and space to establish this instrument at the Foothills Research Campus. The initial service contracts will derive from the guarantee of the machine and thereafter, users' feel will be responsible for the cost of the facility.