Acquisition of Xray Diffraction Instrumentation

描述（由申请人提供）：需要一个布鲁克X射线高强度仪器系统，以实现单晶高分辨率X射线衍射实验，用于确定蛋白质、核酸和高阶组装体的分子结构。该系统结合了一个高频微处理器控制的微焦点旋转阳极X射线发生器，高亮度多层光学提供非常高的单色性，和一个4K电荷耦合器件（CCD）面积探测器与高分辨率的磷适合铜波长。该仪器将为NIH资助的研究人员提供最先进的系统，能够提供内部X射线仪器中可用的最高强度。新系统将为UCSB的化学和生物化学系，分子，细胞和发育生物学系以及材料研究实验室的结构研究提供强大的工具。该仪器将被安置在位于化学和生物化学系四楼的2000平方英尺的实验室中，并将取代1994年购买的现有理学成像板系统，该系统远远落后于该领域现有的最先进技术。该实验室包括用于晶体生长的孵化器和用于在衍射实验之前对晶体进行最佳检查和操作的实验室空间，并作为进一步的共享资源。研究人员将利用该仪器在基础医学的广泛领域进行研究，包括对基因调控至关重要的蛋白质-核酸复合物的结构研究，具有重要生物材料工程应用的不寻常胶原蛋白，对模式生物C中发育程序的展开至关重要的蛋白质。以及参与介导细胞对其环境的反应的双组分信号网络的蛋白质。该仪器将由一名研究人员作为共享设施进行管理，并在由本提案中列出的多名PI组成的内部咨询委员会的指导下进行管理。将建立一个充值系统，以产生用于支付维护费用的资金。相关性声明：获得旋转阳极发生器和最先进的CCD探测器将为UCSB的大量NIH资助的研究人员提供重要工具。这些项目具有基本的健康相关性，包括开发新的蛋白质疗法和潜在的微生物疾病和肿瘤发生过程的治疗干预。

DESCRIPTION (provided by applicant): A Bruker X-ray high-intensity instrumentation system is requested to enable single crystal highresolution Xray diffraction experiments for the determination of the molecular structures of proteins, nucleic acids, and higher-order assemblies. The system combines a high frequency microprocessor-controlled microfocus rotating anode X-ray generator, high-brilliance multilayer optics providing very high monochromaticity, and a 4K charged-couple device (CCD) area detector with high resolution phosphor suitable for copper wavelengths. This instrumentation will provide NIH-funded investigators with a state-of-the-art system capable of delivering the highest intensity available in an in-house Xray instrument. The new system will provide a powerful tool for structural studies to enable the research of PI's in the Chemistry and Biochemistry Department, Molecular, Cellular and Developmental Biology Department, and Materials Research Laboratory at UCSB. The instrument will be housed in a 2000 square-foot laboratory located on the fourth floor of the Chemistry and Biochemistry Department, and will replace an existing Rigaku image-plate system that was purchased in 1994 and is well behind existing state-of-the-art in the field. This laboratory includes incubators for crystal growth and laboratory space for optimal examination and manipulation of crystals prior to the diffraction experiment, and serves as a further shared resource. The investigators will utilize the instrument to conduct research in broad areas of basic medical science, including structural studies of protein-nucleic acid complexes crucial to gene regulation, of unusual collagens with important biomaterials engineering applications, of proteins central to the unfolding of the developmental program in the model organism C. elegans, and of proteins involved in two-component signaling networks mediating the responses of cells to their environments. The instrument will be managed by a staff researcher as a shared facility, under the direction of an internal advisory committee composed of a number of the PI's listed in this proposal. A recharge system will be set up to generate the funds used to cover maintenance costs. Statement of Relevance: The acquisition of a rotating anode generator and state-of-the-art CCD detector will provide a crucial tool to enable the research of a large number of NIH-funded investigators at UCSB. These projects have fundamental health relevance including the development of novel protein-based therapeutics and potential therapeutic interventions into microbial diseases and the process of oncogenesis.