Biacore T100

比亚科T100

• 批准号: 7215088
• 负责人: Charles Joel Arntzen
• 金额: $ 36.61万
• 依托单位: ARIZONA STATE UNIVERSITY-TEMPE CAMPUS
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-03-01 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7215088
• 关键词: Advisory Committees; Agriculture; Arizona; Automation; Award; Binding; Biological; Biology; Chemistry; Complement; Computer software; Conflict (Psychology); Core Facility; DNA laboratory; Data; Discipline; Disease; Ecology; Electronics; Engineering; Faculty; Fees; Grant; Growth; Hour; Housing; Human Resources; Institutes; Malignant Neoplasms; Medicine; Minor; Neurologic; Physics; Postdoctoral Fellow; Protein Chemistry; Public Health; Recruitment Activity; Research; Schedule; Science; Students; Surface Plasmon Resonance; Systems Analysis; Technology; Training; Universities; Wages; biodefense; day; experience; improved; instrument; vaccinology

DESCRIPTION (provided by applicant): This award will support the acquisition of a Biacore T100 molecular interaction analysis system at Arizona State University. The T100 instrument, which utilizes surface plasmon resonance (SPR) technology, will complement the two SPR instruments currently in use on the campus, a Biacore X and a FlexChip, neither of which has the capabilities or capacity to satisfy the growing needs for characterizing molecular interactions at ASU. Growth at ASU has included a net increase of 125 tenured and tenure-track faculty during the past two years with a corresponding growth in scientific research. The Biodesign Institute at Arizona State University is an exciting venture in merging of scientific disciplines, with faculty from the fields of biology, chemistry, physics, medicine, agriculture, environmental science, electronics, materials science, engineering and computing. The Biacore T100 offers the automation, improved software, and sensitivity that is required to take analysis further than is possible with either of the two instruments currently available on campus. The instrument will be housed at The Biodesign Institute, where it will be readily accessible to all PIs whose labs are located in the building, and is in close proximity to PIs located in other buildings. We plan to recruit a postdoctoral fellow with SPR experience to be the manager of the facility. The salary support for the manager will be initially shared by the major users of the instrument, but will eventually be paid from the usage fees generated by the instrument. ASU has several recoverable core facilities on campus which have operated successfully for 5-10 years (such as DNA Laboratory, Protein Chemistry Lab, EM Lab). Scheduling of the instrument will be electronic, and conflicts will be resolved by an advisory committee consisting of the PI of the grant, two major users, and one minor user. Approximately 15% of the available hours will be reserved for new and outside users. We currently have sent 6 graduate students to Biacore for training, and those labs have been able to generate preliminary data since the training. We plan to send additional personnel to training when the instrument has been delivered on campus. Though we have some access to the Biacore X, it is heavily utilized already (greater than 50% by two major users) and cannot fill the needs of our expanding faculty. Preliminary data has also been generated on the FlexChip located on campus, but further analysis requires the greater sensitivity available with a Biocore T100. Our needs have far outgrown the currently available instrument accessibility, and the purchase of the T100 will greatly advance the research schedules. Public Health Relevance: Our herein described projects encompass vaccinology, cancer and disease treatments, neurological therapies, and biodefense; an understanding of how biological molecules interact is a prerequisite to successful pursuit of answers to the relevant problems faced each day by Public Health officials globally.

描述（由申请人提供）：该奖项将支持亚利桑那州立大学收购Biacore T100分子相互作用分析系统。T100仪器，利用表面等离子体共振（SPR）技术，将补充目前在校园使用的两个SPR仪器，一个Biacore X和一个FlexChip，这两个仪器都没有能力或能力来满足亚利桑那州立大学日益增长的分子相互作用的需求。亚利桑那州立大学的增长包括在过去两年中净增加了125名终身教职和终身教职员工，同时科学研究也有相应的增长。亚利桑那州立大学的生物设计研究所是一个令人兴奋的科学学科合并的企业，拥有来自生物学，化学，物理学，医学，农业，环境科学，电子学，材料科学，工程和计算领域的教师。Biacore T100提供了自动化，改进的软件和灵敏度，这是需要采取进一步的分析比任何两个仪器目前在校园里可用。该仪器将存放在生物设计研究所，实验室位于该建筑物内的所有PI都可以轻松访问该仪器，并且该仪器靠近位于其他建筑物内的PI。我们计划招聘一名具有SPR经验的博士后研究员担任该设施的经理。对管理人员的薪金支助最初将由仪器的主要用户分担，但最终将从仪器产生的使用费中支付。亚利桑那州立大学在校园内有几个可恢复的核心设施，这些设施已经成功运行了5-10年（如DNA实验室，蛋白质化学实验室，EM实验室）。仪器的时间安排将是电子的，冲突将由一个咨询委员会解决，该委员会由赠款的PI，两个主要用户和一个次要用户组成。大约15%的可用时间将保留给新用户和外部用户。我们目前已经派出了6名研究生到Biacore进行培训，这些实验室已经能够在培训后生成初步数据。我们计划在仪器送到校园后再派更多的人员参加培训。虽然我们有一些访问Biacore X，它已经被大量使用（两个主要用户超过50%），不能满足我们不断扩大的教师的需求。位于校园内的FlexChip也生成了初步数据，但进一步分析需要Biocore T100提供更高的灵敏度。我们的需求已经远远超出了当前可用仪器的可访问性，购买T100将大大提前研究时间表。公共卫生相关性：我们在此描述的项目包括疫苗学，癌症和疾病治疗，神经疗法和生物防御;了解生物分子如何相互作用是成功寻求全球公共卫生官员每天面临的相关问题的答案的先决条件。