The role of tmRNA in development of C. crescentus

tmRNA 在 C. crescentus 发育中的作用

• 批准号: 7226649
• 负责人: KENNETH C KEILER
• 金额: $ 24.98万
• 依托单位: PENNSYLVANIA STATE UNIVERSITY, THE
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-05-01 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7226649
• 关键词: Affect; Bacteria; Bacteriophages; Binding Proteins; Biology; Caulobacter crescentus; Cell Cycle; Cell Cycle Regulation; Cells; Chromatin Structure; Complex; DNA Replication Factor; DNA Replication Timing; DNA biosynthesis; Data; Defect; Development; Event; Gene Expression; Gene Expression Regulation; Genes; Genetic; Genetic Transcription; Genome; Goals; Human; Knowledge; Mass Spectrum Analysis; Mediating; Messenger RNA; Modeling; Modification; Molecular; Mutation; Organism; Pathogenesis; Pathway interactions; Peptides; Physiological; Plasmids; Process; Property; Proteins; Quality Control; RNA; RNA Degradation; RNA Splicing; Reaction; Regulation; Regulatory Pathway; Replication Initiation; Research; Research Personnel; Ribosomes; Role; Site; Stress; Symbiosis; Testing; Time; Transfer RNA; Translations; Untranslated RNA; Work; base; genetic selection; human disease; interest; mRNA Stability; polypeptide; programs; promoter; research study; ribonuclease R; tmRNA

DESCRIPTION (provided by applicant): Regulation of gene expression by noncoding RNAs (ncRNAs) is an emerging paradigm in biology. ncRNAs regulate a variety of processes, including transcription, translation, RNA modification, mRNA stability, RNA splicing, chromatin structure, and protein stability and activity, in a wide variety of organisms from bacteriophages to humans. Eukaryotic and prokaryotic genomes contain hundreds of uncharacterized ncRNAs, and these genes have been increasingly identified as the loci of mutations that cause developmental defects and several human diseases. One of the most interesting of these ncRNAs is tmRNA, a molecule with properties of both a tRNA and an mRNA, which intervenes in selected translation reactions; tmRNA recognizes selected translation complexes and enters the ribosome to mediate the addition of a peptide tag to the nascent polypeptide, targeting the protein for rapid degradation and releasing the ribosome and mRNA. This unique activity functions both in a translational quality control mechanism and in regulation of gene expression, tmRNA is conserved throughout the bacterial kingdom, and is required for processes such as pathogenesis, symbiosis, stress tolerance, and bacterial development. The long-term objective of this application is to understand the mechanism of action and physiological role of tmRNA in bacteria. This application focuses on the role of tmRNA in the development and cell cycle regulation of Caulobacter crescentus. C. crescentus is the organism of choice for these studies because the extensive knowledge of the molecular events that control its cell cycle and developmental program provide a unique opportunity to determine the influence of tmRNA activity on cellular differentiation and DNA replication, tmRNA is required for proper timing of DNA replication and the coincident differentiation from swarmer cell to stalked cell in C. crescentus. The primary goals of this application are: to identify substrates for tmRNA in C. crescentus that are important for control of DNA replication, to determine how tmRNA activity on these substrates affects DNA replication, and to understand how the activity of tmRNA is controlled by the genetic regulatory network of the cell.

描述（由申请人提供）：通过非编码rna （ncRNAs）调控基因表达是生物学中的一个新兴范例。在从噬菌体到人类的各种生物中，ncRNAs调节多种过程，包括转录、翻译、RNA修饰、mRNA稳定性、RNA剪接、染色质结构、蛋白质稳定性和活性。真核生物和原核生物基因组包含数百个未表征的ncrna，这些基因已越来越多地被确定为导致发育缺陷和几种人类疾病的突变位点。