CORE--ANIMAL

核心--动物

• 批准号: 7215502
• 负责人: JIANMING XU
• 金额: $ 17.5万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-01 至 2011-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7215502
• 关键词: animal colony; biomedical facility; biotechnology; gene induction /repression; gene targeting; genetic manipulation; genetic models; genetically modified animals; laboratory mouse; model design /development; transcription factor

In the last five-year funding period, the investigators of this Program Project Grant (PPG) have focused on the studies for characterizing the genetic and metabolic fingerprints of the three members of the p160 steroid receptor coactivator (SRC) family. In these studies, genetically manipulated mouse models have being extensively used. The Animal Core has met the needs of individual investigators of this PPG by generating a number of new transgenic mouse lines, providing excellent mouse services and sufficient number of genetically manipulated mice for their experiments. In the current proposal, the investigators of this PPG have proposed extensive experiments that are heavily dependent on genetically manipulated mouse models and thereby the role of the Animal Core becomes even more critical than before to the continued success of this PPG. In this proposal, the Animal Core will continue to provide the Investigators of this PPG with the ability to utilize genetically manipulated mouse models and to generate new transgenic mouse lines. In particular, the Animal Core will maintain mouse lines that were produced and used in the last funding period and continuously needed for the next funding cycle, including SRC-1, SRC-2 and SRC-3 knockout mouse lines; the Animal Core will import and re-derive several mouse lines into the Baylor Mouse Facility from other institutes to meet the needs of individual projects of this PPG, including the floxed SRC-1 and the floxed SRC-3 mice; and the Animal Core will generate new transgenic mouse lines to satisfy the needs of individual investigators for their studies with spatial and temporal inactivation of specific genes in the adipose tissue, liver, skeletal muscle and cultured cells derived from our mutant/transgenic mice. Furthermore, the Animal Core will breed, produce and provide age, sex and genotype-matched mice to individual investigators for their experiments. In addition, the Animal Core will serve as a resource for execution and training in the use of all animal manipulations needed in this PPG proposal. The Animal Core will minimize the cost of utilizing animal models in this PPG by consolidating the equipment, expertise and animal resources, ensuring an efficient use of animal resources at minimal cost.

在过去的五年资助期间，该计划项目赠款（PPG）的调查人员重点关注 用于表征P160类固醇的三个成员的遗传和代谢指纹的研究 受体辅激活因子（SRC）家族。在这些研究中，基因操作的小鼠模型已经被 广泛使用。动物中心通过生成以下内容满足了本PPG个体研究者的需求： 一批新的转基因小鼠品系，提供优良的小鼠服务和足够数量的 基因改造的老鼠来做实验在当前提案中，PPG的研究者 已经提出了大量的实验，这些实验严重依赖于基因操纵的小鼠模型 因此，动物核心的作用变得比以前更加关键， 这个PPG。在本提案中，动物中心将继续向本PPG的研究者提供 利用基因操作的小鼠模型和产生新的转基因小鼠品系的能力。在 特别是，动物中心将保留在上一个资助期内生产和使用的小鼠品系 并持续需要下一个资金周期，包括SRC-1，SRC-2和SRC-3敲除小鼠 动物核心将导入并重新衍生几个小鼠品系到贝勒小鼠设施从其他 研究所，以满足本PPG的个别项目的需要，包括浮动SRC-1和浮动 SRC-3小鼠;动物核心将产生新的转基因小鼠品系，以满足 个别研究人员对脂肪中特定基因的空间和时间失活的研究 组织、肝脏、骨骼肌和来自我们的突变/转基因小鼠的培养细胞。而且 Animal Core将繁殖、生产并向个体研究者提供年龄、性别和基因型匹配的小鼠 进行实验此外，动物核心将作为执行和培训的资源， 使用本PPG提案中所需的所有动物操作。动物核心将最大限度地减少 通过整合设备、专业知识和动物资源，在本PPG中利用动物模型， 确保以最低成本有效利用动物资源。