CORE--ANIMAL

核心--动物

• 批准号: 7215502
• 负责人: JIANMING XU
• 金额: $ 17.5万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-01 至 2011-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7215502
• 关键词: animal colony; biomedical facility; biotechnology; gene induction /repression; gene targeting; genetic manipulation; genetic models; genetically modified animals; laboratory mouse; model design /development; transcription factor

In the last five-year funding period, the investigators of this Program Project Grant (PPG) have focused on the studies for characterizing the genetic and metabolic fingerprints of the three members of the p160 steroid receptor coactivator (SRC) family. In these studies, genetically manipulated mouse models have being extensively used. The Animal Core has met the needs of individual investigators of this PPG by generating a number of new transgenic mouse lines, providing excellent mouse services and sufficient number of genetically manipulated mice for their experiments. In the current proposal, the investigators of this PPG have proposed extensive experiments that are heavily dependent on genetically manipulated mouse models and thereby the role of the Animal Core becomes even more critical than before to the continued success of this PPG. In this proposal, the Animal Core will continue to provide the Investigators of this PPG with the ability to utilize genetically manipulated mouse models and to generate new transgenic mouse lines. In particular, the Animal Core will maintain mouse lines that were produced and used in the last funding period and continuously needed for the next funding cycle, including SRC-1, SRC-2 and SRC-3 knockout mouse lines; the Animal Core will import and re-derive several mouse lines into the Baylor Mouse Facility from other institutes to meet the needs of individual projects of this PPG, including the floxed SRC-1 and the floxed SRC-3 mice; and the Animal Core will generate new transgenic mouse lines to satisfy the needs of individual investigators for their studies with spatial and temporal inactivation of specific genes in the adipose tissue, liver, skeletal muscle and cultured cells derived from our mutant/transgenic mice. Furthermore, the Animal Core will breed, produce and provide age, sex and genotype-matched mice to individual investigators for their experiments. In addition, the Animal Core will serve as a resource for execution and training in the use of all animal manipulations needed in this PPG proposal. The Animal Core will minimize the cost of utilizing animal models in this PPG by consolidating the equipment, expertise and animal resources, ensuring an efficient use of animal resources at minimal cost.

在过去的五年资助期内，该计划项目赠款(PPG)的调查人员专注于 P160类固醇三种成员的遗传和代谢指纹图谱特征研究 受体辅活化子(SRC)家族。在这些研究中，基因操纵的小鼠模型 被广泛使用。动物核心满足了PPG个人调查人员的需求，通过生成 一批新的转基因小鼠品系，提供优质的小鼠服务和足够数量的 为他们的实验进行基因操纵的小鼠。在目前的提案中，这一PPG的调查人员 提出了广泛的实验，这些实验严重依赖于基因操纵的小鼠模型 因此，动物核心的作用变得比以前更加关键，对于继续取得成功 这个PPG。在这项提案中，动物核心将继续为这一PPG的调查人员提供 能够利用基因操纵的小鼠模型并产生新的转基因小鼠品系。在……里面 特别是，动物核心将维护在上一个资助期生产和使用的老鼠品系 并为下一个供资周期持续需要，包括SRC-1、SRC-2和SRC-3基因敲除小鼠 线；动物核心将导入和重新派生几个鼠标线到贝勒老鼠设施从其他 各研究所以满足这一PPG个别项目的需要，包括FLOLED SRC-1和FLOXED SRC-3小鼠；动物核心将产生新的转基因小鼠品系，以满足 研究脂肪中特定基因的空间和时间失活的个体研究者 来自我们的突变/转基因小鼠的组织、肝脏、骨骼肌和培养细胞。此外， 动物核心公司将培育、生产和向个体研究人员提供年龄、性别和基因匹配的小鼠 用于他们的实验。此外，动物核心将作为执行和培训的资源 使用PPG提案中所需的所有动物操作。动物核心将最大限度地减少 通过整合设备、专业知识和动物资源，在这个PPG中利用动物模型， 确保以最低成本有效利用动物资源。