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Core B (Animal Component)

核心B（动物成分）

基本信息

批准号：
10421280
负责人：
JIANMING XU
金额：
$ 23.78万
依托单位：
BAYLOR COLLEGE OF MEDICINE
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-07-01 至 2024-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10421280
关键词：
Adipose tissue Animal Experiments Animal Husbandry Animals Breeding CRISPR/Cas technology Caring Cells Collaborations DNA Data Disease Embryo Embryo Transfer Equipment FOXO1A gene Feeding behaviors Female Fertilization in Vitro Fostering Functional disorder Funding Gene Targeting Generations Genes Genetically Engineered Mouse Genotype Guidelines Homeostasis Human Human Resources Hypothalamic structure Institutes Knock-in Knock-out Liver Maintenance Mediating Mediator of activation protein Messenger RNA Metabolic Metabolic Diseases Metabolic Pathway Metabolism Methodology Methods Molecular Target Mus Mutant Strains Mice Nuclear Receptors Ovulation PPAR alpha PPAR gamma Pathway interactions Physiological Production Program Effectiveness Program Research Project Grants Protein Isoforms Proteins Protocols documentation Regulator Genes Research Research Personnel Resources Role STAT3 gene Savings Services Site System Technology Therapeutic Time Training Transgenes Transgenic Mice Transgenic Organisms United States National Institutes of Health Variant XBP1 gene animal facility animal resource base conditional knockout cost effectiveness embryo cryopreservation embryonic stem cell experience experimental analysis experimental study genotypic sex homologous recombination knock-down male mouse genome mouse model nuclear receptor coactivator 1 operation overexpression pre-clinical preservation programs sex sperm cryopreservation success transcription factor

项目摘要

Core B (Animal Component) - Project Summary All research projects of this Program Project (PP) will rely on the use of genetically engineered mouse (GEM) models to investigate the roles and mechanism of Nuclear Receptors, transcription factors and their coregulators in energy and metabolic homeostasis and disease. More specifically, at least 34 GEM mouse lines will be used, and thousands of male and female mice will be produced by breeding in house during the funding period of this PP. Therefore, this Animal Component of Core B is essential for the success of this PP by integrating expensive equipment, advanced technology, experienced personnel and animal resources. As shown by preliminary studies, Core B has generated/established multiple GEM lines and developed CRISPR-Cas9-mediated mouse gene editing systems. Core B is dedicated to achieving 3 major objectives. Firstly, Core B will use its specialized expertise, state-of-the-art equipment and adequate facilities to generate GEM lines for all investigators of this PP. Core B has established the necessary methods to edit the mouse genome by homologous recombination- based gene targeting in ES cells or CRISPR/Cas9-based gene editing in 1-cell mouse embryos. Core B will generate all GEM lines needed by the investigators of this PP. Secondly, Core B will provide high quality mouse services to manage GEM line colonies and produce genotype-defined and age/sex-matched mice for all investigators of this PP. Specifically, Core B will breed and maintain all of the 34 mouse lines used by this PP. Core B will use our colony expansion protocol to produce large numbers of genotype/age/sex-matched mice for experimental analysis. Core B also will perform genotype analysis, super ovulation, embryo and sperm cryopreservation, and in vitro fertilization services to help the investigators of this PP for managing their mouse colonies, preserving GEM lines, transferring mouse lines between institutes, and saving animal husbandry expenses. Finally, Core B will integrate the efficient usage of animal resources by all investigators of this PP. Because of the large-scale operation of mouse models and the sharing features of multiple mouse lines among the projects of this PP, centralized integration of equipment, expertise and valuable mouse resource will be essential for both scientific success and cost effectiveness. Core B will minimize the expenses of animal experiments by consolidating the usage and management of equipment, expertise, animal facility, mouse maintenance and production. In addition, Core B will serve as a resource for execution and technical training in the use of all animal manipulations needed and oversee the care and use of experimental mice for this PP.

核心B（动物成分）——项目总结