Let your metabolites shine: new NMR experiments and hardware for enhanced studies of metabolites

让您的代谢物大放异彩：新的 NMR 实验和硬件可增强代谢物的研究

• 批准号: 2898065
• 金额: --
• 依托单位: University of Manchester
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2023
• 资助国家: 英国
• 起止时间: 2023 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2898065
• 关键词: Let; your; metabolites; shine; new

"Nuclear magnetic resonance spectroscopy (NMR) is increasingly applied in studies of metabolites because it is arguably one of the most powerful methods for obtaining structural and dynamic information in complex mixtures. Comprehensively monitoring the patterns of metabolites can be useful to both the analysis of underlying metabolite networks (e.g. the responses to the changing environment or the presence of stress, pest or disease) and as metabolite fingerprinting approaches (e.g., to confirm a food product's authenticity or to demonstrate lack of potentially toxic adulteration [1]). One exciting possibility to further improve the value of NMR-based metabolomics - which is still lacking sensitivity compared to conventional mass spectrometry approaches - is to take advantage of photo-chemically induced dynamic nuclear polarisation (photo-CIDNP [2]), where observed NMR signal intensities of aromatic compounds in the presence of a photosensitiser are modulated and often significantly increased upon illumination. We recently proposed and patented the new way of illuminating NMR samples in situ using LEDs (NMRtorch approach [3,4]), leading to photo-CIDNP enhancements exceeding those obtained using lasers, while allowing for potentially much higher sample throughput, and even automation. The aim of the proposed project is to explore the use of photo-CIDNP for studies of aromatic metabolites, initially for the purpose of fingerprinting and authentication of plant-based liquid food products, such as grape juice or wine, and then extending it to other metabolomics systems such as biological fluids, for example for identifying and monitoring metabolite biomarkers associated with health and disease. "

“核磁共振光谱（NMR）越来越多地应用于代谢物的研究，因为它可以说是获得复杂混合物结构和动力学信息的最有力的方法之一。全面监测代谢物的模式对于分析潜在的代谢物网络（例如，对变化的环境或压力、害虫或疾病的存在的响应）和作为代谢物指纹识别方法（例如，确认食品的真实性或证明没有潜在的有毒掺假[1]）。进一步提高基于NMR的代谢组学价值的一个令人兴奋的可能性-与传统质谱方法相比仍然缺乏灵敏度-是利用光化学诱导的动态核极化（photo-CIDNP [2]），其中在光敏剂存在下观察到的芳香族化合物的NMR信号强度被调制，并且通常在照明时显着增加。我们最近提出并申请了使用LED原位照明NMR样品的新方法（NMRtorch方法[3，4]），导致光CIDNP增强超过使用激光获得的增强，同时允许潜在的更高的样品吞吐量，甚至自动化。拟议项目的目的是探索使用photo-CIDNP研究芳香代谢物，最初用于植物液体食品（如葡萄汁或葡萄酒）的指纹识别和认证，然后将其扩展到其他代谢组学系统，如生物液体，例如用于识别和监测与健康和疾病相关的代谢物生物标志物。"