CORE--RESEARCH

核心--研究

• 批准号: 7266247
• 负责人: JUAN CAPASSO
• 金额: $ 12.34万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7266247
• 关键词: arginine vasopressin; biological signal transduction; biomedical facility; genetic regulation; health science research support; tissue /cell culture; vasopressins

The research core laboratory, Core B, directed by Dr. Juan Capasso, is a resource facility located within the Division of Renal Diseases and Hypertension at the University of Colorado Health Sciences Center. it is comprised of laboratories set up as a core facility for all the investigators of the program project. The research core provides access to the necessary general equipment and supplies shared by all of the investigators of the program project. Specifically, the Core B includes a tissue culture facility/laboratory (Milli-Q water system, CO2 incubators, laminar flow hoods, microscopes, cryostorage, supplies/reagents), large equipment rooms (spectrophotometers, refrigerators, freezers, pH meters, centrifuges, film developer, PCR, analyzers and electrophoresis equipment), a radioactivity laboratory (beta and gamma counters and radioactive waste disposal containers), a dishwashing room (drying oven, autoclave etc), and an animal surgery laboratory (dissecting scopes, surgical instruments and supplies, scales, pumps, metabolic cages, restraining devices, incubator, small and micro hematocrit centrifuges, heated surgical tables, small animal blood flow meter/renal blood flow and blood pressure system, tail cuff blood pressure analyzer). In addition, an inventory of standard chemicals/reagents, glassware and expendable supplies will be maintained for use by all the investigators. This core will also support the service contracts and repair of these facilities.

由Juan Capasso博士指导的研究核心实验室Core B是位于科罗拉多大学健康科学中心肾脏疾病和高血压部的资源设施。它由实验室组成，作为方案项目所有调查人员的核心设施。研究核心提供了必要的通用设备和用品共享的程序项目的所有研究人员。具体而言，核心B包括组织培养设施/实验室（Milli-Q水系统、CO2培养箱、层流罩、显微镜、冷冻库、供应品/试剂）、大型 设备室（温度计、冰箱、冷冻机、pH计、离心机、胶片显影机、聚合酶链式反应器、分析仪和电泳设备）、放射性实验室（β和γ计数器和放射性废物处置容器）、洗碗间（干燥箱、高压灭菌器等）和动物外科实验室（解剖镜、手术器械和用品、天平、泵、代谢笼、约束装置、培养箱、小型和微型血细胞比容离心机、加热手术台，小动物血流量计/肾血流量和血压系统，尾袖血压分析仪）。此外，还将维持标准化学品/试剂、玻璃器皿和消耗性用品的库存，以供使用 所有的调查员。这一核心还将支持这些设施的服务合同和维修。