Sensing and regulation for autonomous biomanufacturing control

自主生物制造控制的传感和调节

• 批准号: 2898939
• 金额: --
• 依托单位: University of Manchester
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2023
• 资助国家: 英国
• 起止时间: 2023 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2898939
• 关键词: Sensing; regulation; autonomous; biomanufacturing; control

Synthetic biology and metabolic engineering offer methods to develop new biosynthetic routes and hosts for the production of chemicals, biopolymers and fuels. As such we can reduce our reliance on petrol-chemical based feedstocks by providing new sustainable production routes for drop in chemicals and develop new chemical entities with advanced properties. A major challenge with biomanufacturing from microbial host is the need to balance the cells viability/growth with the biotechnological production demands. For example, high-producing clones can often suffer from limited growth, due to the burden/demand of recombinant production, and thus lead to low overall titres. This PhD project will seek to develop genetic tools to sense and regulate molecular signals and genes respectively, to permit Autonomous Biomanufacturing Control. This will involve transcriptomics (temporal expression analysis), bioinformatics, promoter engineering (library generation, high-throughput screening and sequence-function analysis), and temporal promoter development (generation of design rules, performance optimisation and application). This project and training will equip you with skills in advanced synthetic biology, data mining and analysis suitable for future careers in both academia or industry.

合成生物学和代谢工程为开发新的生物合成路线和宿主提供了方法，用于生产化学品、生物聚合物和燃料。因此，我们可以通过为化学品的下降提供新的可持续生产路线和开发具有先进性能的新化学实体来减少对石油化工原料的依赖。微生物宿主生物制造的一个主要挑战是需要平衡细胞活力/生长与生物技术生产需求。例如，由于重组生产的负担/需求，高产克隆往往生长有限，从而导致总滴度低。这个博士项目将寻求开发基因工具来分别感知和调节分子信号和基因，以实现自主生物制造控制。这将涉及转录组学（时间表达分析）、生物信息学、启动子工程（文库生成、高通量筛选和序列功能分析）和时间启动子开发（设计规则生成、性能优化和应用）。该项目和培训将为您提供先进的合成生物学，数据挖掘和分析技能，适合未来学术界或工业界的职业生涯。