A Microfluidic Platform for Dental Biofilm Growth and Characterization

用于牙科生物膜生长和表征的微流体平台

基本信息

批准号：
7268075
负责人：
Todd A Thorsen
金额：
$ 14.79万
依托单位：
MASSACHUSETTS INSTITUTE OF TECHNOLOGY
依托单位国家：
美国
项目类别：
财政年份：
2006
资助国家：
美国
起止时间：
2006-08-01 至 2009-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7268075
关键词：
Actinomyces viscosus Adhesions Aerobic Aerobic Bacteria Anaerobic Bacteria Architecture Artificial Saliva Artificial Tooth Bacteria Campylobacter Campylobacter rectus Condition Culture Media Dental Dental Hygiene Dental caries Devices Diffusion Dimensions Fluorescence Fluorescent Antibody Technique Fusobacterium nucleatum Future Gases Glycoproteins Goals Gram-Positive Bacteria Growth Heart Diseases Hydroxyapatites In Vitro Ion Channel Mechanics Methods Microbial Biofilms Microfluidic Microchips Microfluidics Modification Molds Monitor Morphology Mucins Nitrogen Oral cavity Organ failure Oxygen Pattern Permeability Pit and Fissure Sealants Porphyromonas gingivalis Prevotella intermedia Process Rate Research Research Personnel Sampling Secondary to Series Silicon Silicone Elastomers Standards of Weights and Measures Streptococcus mutans Structure System Techniques Thick Time Tooth Sealants Tooth structure Visible Radiation base density design elastomeric in vivo innovation light microscopy polydimethylsiloxane programs research study sensor size small molecule libraries tool tooth surface

项目摘要

DESCRIPTION (provided by applicant): This proposal outlines a project geared toward the design of a microfluidic "mouth-on-a-chip" system to study dental biofilm formation, a process associated not only with tooth decay, but also more serious systemic problems such as heart disease and organ failure if not kept under control through regimented oral hygiene. By varying the input oxygen/nitrogen ratio, sample flow rates, and the attachment substrate structure and composition (sealants, binders), the microfluidic chips will function as very powerful, inexpensive tools to study differential biofilm formation under a matrix of environmental conditions. Once validated, future research with the chips will focus on investigating mechanisms to disrupt the biofilm formation, including modification of the dental sealant composition, and the use of small molecule libraries to block intrabacterial interactions.

描述（由申请人提供）：该提案概述了一个针对设计微流体“ chip-a-a-a-chip”系统的项目，用于研究牙科生物膜形成，这不仅与牙齿衰减相关，而且还具有更严重的全身性问题，例如心脏病和器官，如果无法通过管制的口腔卫生进行控制，则是更严重的系统性问题。通过改变输入氧/氮比，样品流速以及附着的底物结构和成分（密封剂，粘合剂），微流体芯片将作为在环境条件矩阵下研究差异性生物膜形成的非常强大，廉价的工具。一旦经过验证，未来对芯片的研究将集中在研究破坏生物膜形成的机制上，包括修饰牙齿密封剂组成，以及使用小分子文库来阻止细菌内相互作用。