Evolution of Abalone Egg Coat Proteins

鲍鱼蛋壳蛋白的进化

• 批准号: 7276423
• 负责人: Jan E Aagaard
• 金额: $ 4.96万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2008-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7276423
• 关键词: Address; Affinity; Amino Acid Sequence; Animal Model; Animals; Area; Base Sequence; Binding; Biochemical; California; Capsid Proteins; Cell membrane; Cytoplasmic Granules; Development; Egg Proteins; Evolution; Exhibits; Fertilization; Fractionation; Genetic Polymorphism; Genomics; Human; Immunoprecipitation; Ligands; Light; Linkage Disequilibrium; Mammals; Marines; Mass Spectrum Analysis; Mediating; Membrane; Molecular; Pattern; Proteins; Proteomics; Relative (related person); Reproduction; Reproductive Biology; Research; Specificity; Sperm-Ovum Interactions; Statistical Methods; System; Taxon; Testing; Thinking; Training; Vertebrates; Work; abalone; base; driving force; egg; lysin; novel; paralogous gene; receptor; receptor binding; reproductive; research study; sperm cell; sperm protein

DESCRIPTION (provided by applicant): This proposal is aimed at furthering our understanding of the interactions between sperm and egg proteins, and the evolutionary forces driving reproductive protein evolution. The work employs the abalone system, which provides one of the most extensively studied sperm-egg interaction systems and which is amenable to biochemical and experimental studies difficult in other taxa. Two specific aims are proposed: (1) identify and characterize the egg plasma-membrane bound receptor of the abalone sperm protein sp18; and (2) characterize polymorphism and divergence of a candidate sp18 receptor already identified (ZPD) within and among abalone species, and test for co-evolution with sp18. Specific Aim 1 utilizes biochemical approaches (immunoprecipitation and affinity columns) and mass spectrometry to identify proteins from fractionated egg components which bind with high specificity to sp18. This work will test for evidence of direct functional interactions between sp18 and egg molecules. Specific Aim 2 examines polymorphism at a candidate sp18 receptor locus (ZPD) identified in genomic screens for egg coat proteins, as well as divergence at this locus among California abalone species. Patterns of polymorphism and sequence divergence for ZPD will be tested for evidence of positive selection as well as coevolution with sp18. Because these signatures are predicted for the sp18 receptor, they provide an independent and parallel means of distinguishing among candidate egg receptors (including ZPD and any others which may be identified under Specific Aim 1). Together these research aims represent a significant advancement in our understanding of the molecular interactions between sperm and egg, and contribute to a broader understanding of reproductive biology including human reproduction. The interactions between sperm and egg molecules during fertilization have proven elusive in studies of human reproduction. The model organism used in this study (abalone, a marine gastropod) shares a common molecular basis with vertebrates (including humans and other mammals), exhibits a common pattern of rapid divergence of sperm and egg proteins, and allows for experimental approaches not possible in other animals. Thus the proposed work addresses general themes in reproductive biology, and may shed light on the molecular basis of sperm and egg interactions of relevance to human reproduction.

描述（由申请人提供）：该提案旨在进一步了解精子和卵子蛋白之间的相互作用，以及驱动生殖蛋白进化的进化力量。这项工作采用了鲍鱼系统，它提供了一个最广泛的研究精卵相互作用系统，这是服从生化和实验研究困难，在其他类群。提出了两个具体的目标：（1）确定和表征鲍鱼精子蛋白sp18的卵质膜结合受体;（2）表征鲍鱼物种内和物种间已确定的候选sp18受体（ZPD）的多态性和趋异性，并测试与sp18的共进化。Specific Aim 1利用生物化学方法（免疫沉淀和亲和柱）和质谱法从分离的鸡蛋成分中鉴定出与sp18高度特异性结合的蛋白质。这项工作将测试sp18和卵分子之间直接功能相互作用的证据。具体目标2检查多态性在一个候选的sp18受体基因座（ZPD）确定在基因组筛选卵外壳蛋白，以及在这个位点之间的分歧加州鲍鱼物种。将测试ZPD的多态性和序列趋异模式，以获得正选择以及与sp18共同进化的证据。因为这些特征是针对sp18受体预测的，所以它们提供了区分候选卵受体（包括ZPD和可能在特定目标1下鉴定的任何其他受体）的独立和平行的方法。这些研究目标共同代表了我们对精子和卵子之间分子相互作用的理解的重大进步，并有助于更广泛地理解生殖生物学，包括人类生殖。 受精过程中精子和卵子分子之间的相互作用在人类生殖研究中被证明是难以捉摸的。本研究中使用的模式生物（鲍鱼，一种海洋腹足动物）与脊椎动物（包括人类和其他哺乳动物）具有共同的分子基础，表现出精子和卵蛋白快速分化的共同模式，并允许在其他动物中不可能的实验方法。因此，拟议的工作涉及生殖生物学的一般主题，并可能揭示与人类生殖相关的精子和卵子相互作用的分子基础。