CORE--RADIOIMMUNOASSAY/MONOCLONAL ANTIBODIES

核心--放射免疫分析/单克隆抗体

• 批准号: 7311617
• 负责人: PATRICK SLUSS
• 金额: $ 10.29万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7311617
• 关键词: biomarker; biomedical facility; diabetes mellitus; immunologic assay /test; immunologic substance development /preparation; method development; monoclonal antibody; radioimmunoassay

In its broadest mission, the Immunoassay Core provides technical support and services for the application and innovation of immunoassays. These activities serve as a critical link between basic and clinical investigators. Members of BADERC can access state-of-the-art commercial immunoassays for measuring a wide range of analytes. Many of these are fully automated, high speed systems that can support very large testing volumes and multi-site studies. Importantly, the Immunoassay Core is experienced in adapting these systems to measurements using animal serum or plasma (rats and mice.) The Core also provides access to radioimmunoassay that has been established for many years in Dr Habener's laboratory for metabolic studies in animals and humans. Access to well validated and standardized assays provides interpretive power based upon large normative and pathophysiologic databases thus serving as a crucial backdrop for clinical investigations. The effort to translate basic research into clinical advances benefits by our ability to expedite the transfer of newly identified biomarkers into novel assays that meet the need for the high volume, precise, and sensitive clinical measurements needed to objectively assess the value of new biomarkers. As investigators increasingly focus upon the genetic basis of human disease, an effective mechanism is needed to provide the requisite analytic methods to characterize phenotypic correlates and to assess the incidence of specific genetic-based defects in clinical (e.g., human) and animal models. The Immunoassay Core provides monoclonal facilities and expertise in the generation of antibodies for immunoassay and reagents.

在其最广泛的使命中，免疫测定核心为以下方面提供技术支持和服务： 免疫分析的应用和创新。这些活动是基础研究者和临床研究者之间的重要联系。BADERC的成员可以使用最先进的商业免疫测定来测量各种分析物。其中许多是全自动的高速系统，可以支持非常大的测试量和多站点研究。重要的是，免疫测定核心在调整这些系统以使用动物血清或血浆（大鼠和小鼠）进行测量方面经验丰富。核心还提供了进入放射免疫测定，已建立了多年的哈伯纳博士的实验室，在动物和人类的代谢研究。获得经过充分验证和标准化的检测方法可提供基于大型规范和病理生理数据库的解释能力，因此可作为临床研究的重要背景。将基础研究转化为临床进展的努力得益于我们加快将新鉴定的生物标志物转化为新型检测方法的能力，这些检测方法满足了客观评估新生物标志物价值所需的高容量、精确和灵敏的临床测量的需求。随着研究人员越来越关注人类疾病的遗传基础，需要一种有效的机制来提供必要的分析方法来表征表型相关性并评估临床中特定的基于遗传的缺陷的发生率（例如，人）和动物模型。免疫分析核心提供 单克隆设施和专业知识，在生产抗体的免疫测定和试剂。