CORE--RADIOIMMUNOASSAY/MONOCLONAL ANTIBODIES

在其最广泛的使命中，免疫分析核心为 免疫分析的应用与创新。这些活动是基础研究人员和临床研究人员之间的关键纽带。BADERC的成员可以使用最先进的商业免疫分析方法来测量各种分析物。其中许多都是完全自动化的高速系统，可以支持非常大的测试量和多站点研究。重要的是，免疫分析核心在调整这些系统以适应使用动物血清或血浆(大鼠和小鼠)的测量方面经验丰富。核心还提供了多年来在哈伯纳博士的实验室建立的用于动物和人类新陈代谢研究的放射免疫分析。获得经过良好验证和标准化的分析提供了基于大型规范和病理生理学数据库的解释力，从而为临床研究提供了关键的背景。将基础研究转化为临床进展的努力得益于我们有能力加快将新确定的生物标记物转移到新的分析中，以满足客观评估新生物标记物的价值所需的大量、准确和灵敏的临床测量的需要。随着研究人员越来越关注人类疾病的遗传基础，需要一种有效的机制来提供必要的分析方法来表征表型相关性，并评估临床(例如人类)和动物模型中特定的基于遗传的缺陷的发生率。免疫分析核心提供 在免疫分析和试剂的抗体生成方面的单克隆设备和专业知识。

In its broadest mission, the Immunoassay Core provides technical support and services for the application and innovation of immunoassays. These activities serve as a critical link between basic and clinical investigators. Members of BADERC can access state-of-the-art commercial immunoassays for measuring a wide range of analytes. Many of these are fully automated, high speed systems that can support very large testing volumes and multi-site studies. Importantly, the Immunoassay Core is experienced in adapting these systems to measurements using animal serum or plasma (rats and mice.) The Core also provides access to radioimmunoassay that has been established for many years in Dr Habener's laboratory for metabolic studies in animals and humans. Access to well validated and standardized assays provides interpretive power based upon large normative and pathophysiologic databases thus serving as a crucial backdrop for clinical investigations. The effort to translate basic research into clinical advances benefits by our ability to expedite the transfer of newly identified biomarkers into novel assays that meet the need for the high volume, precise, and sensitive clinical measurements needed to objectively assess the value of new biomarkers. As investigators increasingly focus upon the genetic basis of human disease, an effective mechanism is needed to provide the requisite analytic methods to characterize phenotypic correlates and to assess the incidence of specific genetic-based defects in clinical (e.g., human) and animal models. The Immunoassay Core provides monoclonal facilities and expertise in the generation of antibodies for immunoassay and reagents.