MOLECULAR BIOLOGY

分子生物学

• 批准号: 7002016
• 负责人: LEE MICHAEL KAPLAN
• 金额: $ 15.73万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-01-01 至 2010-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7002016
• 关键词: RNA interference; biomedical facility; gastrointestinal epithelium; gene targeting; genetic library; genetic manipulation; genetically modified animals; immune response; inflammatory bowel diseases; laboratory mouse; leukocyte activation /transformation; microarray technology; molecular biology; polymerase chain reaction; site directed mutagenesis

The major roles of the Molecular Biology Core are (1) to provide services in the utilization of molecular biological techniques and (2) to facilitate the implementation of advances in molecular biology to IBD research. Application of molecular biological approaches is central to the evaluation of processes underlying the development of IBD according to the hypotheses that serve as a foundation for this Center. These approaches include evaluation of the mechanisms for maintaining epithelial function and integrity, factors regulating immune activation and processes contributing to amplification of inflammatory responses and tissue injury [1]. Most importantly, molecular biological approaches are essential in the study of innate immunity, host:microbial interactions at the mucosal interface, dendritic cell/immune cell interactions, human genetic evaluation and murine models of IBD, areas which form key components of IBD-related research in the next support period[2-8]. It is apparent from the description of the CSIBD research base that most Center investigators use basic molecular biological techniques as a fundamental component of their research projects. These techniques permit the identification and characterization of genes regulating epithelial and immune cell function, analysis of the expression of these genes, determination of the functions aid interactions of tie encoded proteins, expression of reporter genes and proteins to allow cellular localization and physiological analysis.

分子生物学核心的主要作用是（1）在分子生物学利用中提供服务 技术和（2）促进IBD研究中分子生物学进步的实施。应用 分子生物学方法的评估是IBD发展基础的过程的核心 根据该中心基础的假设。这些方法包括评估 维持上皮功能和完整性的机制，调节免疫激活和过程的因素 有助于扩增炎症反应和组织损伤[1]。最重要的是分子 生物学方法对于先天免疫的研究至关重要，宿主：粘膜的微生物相互作用 界面，树突状细胞/免疫细胞相互作用，人类遗传评估和IBD的鼠模型，地区 在下一个支持期[2-8]中，哪个是与IBD相关研究的关键组成部分。从 CSIBD研究基础的描述，大多数中心研究人员都使用基本的分子生物学技术作为一种 其研究项目的基本组成部分。这些技术允许识别和表征 调节上皮和免疫细胞功能的基因，这些基因表达的分析，确定 功能有助于TIE编码蛋白的相互作用，报告基因和蛋白质的表达，以允许细胞定位和生理分析。