MOLECULAR BIOLOGY

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The major roles of the Molecular Biology Core are (1) to provide services in the utilization of molecular biological techniques and (2) to facilitate the implementation of advances in molecular biology to IBD research. Application of molecular biological approaches is central to the evaluation of processes underlying the development of IBD according to the hypotheses that serve as a foundation for this Center. These approaches include evaluation of the mechanisms for maintaining epithelial function and integrity, factors regulating immune activation and processes contributing to amplification of inflammatory responses and tissue injury [1]. Most importantly, molecular biological approaches are essential in the study of innate immunity, host:microbial interactions at the mucosal interface, dendritic cell/immune cell interactions, human genetic evaluation and murine models of IBD, areas which form key components of IBD-related research in the next support period[2-8]. It is apparent from the description of the CSIBD research base that most Center investigators use basic molecular biological techniques as a fundamental component of their research projects. These techniques permit the identification and characterization of genes regulating epithelial and immune cell function, analysis of the expression of these genes, determination of the functions aid interactions of tie encoded proteins, expression of reporter genes and proteins to allow cellular localization and physiological analysis.