MOLECULAR AND EXPERIMENTAL PATHOLOGY CORE

芝加哥大学分子实验病理学中心为这项研究提供了至关重要的服务 肠道疾病，特别是免疫介导性、传染性、肿瘤性和炎症性肠道疾病 和动物组织。最简单地说，核心组成部分可以分为四个主要方面：；：(1)人和动物 (主要是小鼠)组织库，(2)解剖病理学审查和会诊以及组织分析(包括 免疫组织化学、激光捕获显微切割、组织阵列)，以及(3)动物使用和设计指南 肠道疾病的结节和(4)流式细胞术。分子实验病理学核心是通过以下方式创建的 2003年重组了分子病理学核心，以更好地满足发改委的需要。这个 之前的核心提供了专门的分子技术、基因工程方面的培训和直接服务 自体和胚胎干细胞系，获得人体组织和对这些组织进行遗传分析的机会有限。 然而，由于大学核心服务在上一个供资期间进行了重组，rbr是一个极好的例子 芝加哥大学转基因/基因敲除小鼠核心已经建立，科学上的进步，许多 提供的专门分子技术已经成为个人很容易完成的常规方法 研究人员实验室或通过芝加哥大学的其他核心，例如DNA测序CON；中国农村合作与发展中心概况 确定最能支持和扩展其工作的服务的成员身份导致了对访问的新关注 人类和动物组织、获得先进工具的机会、使用这些工具的培训、数据解释的专门知识以及 帮助老鼠的使用，而不是发展。如果可能，分子实验病理学核心 与现有资源合作，为核心成员和复员方案协调委员会成员节省了大量成本。 分子实验病理学核心行政主任杰罗尔德·特内特博士医学博士负责监督 手术的所有组成部分和直接参与的所有人体组织相关的手术，提供了解剖学基础 病理检查和咨询服务，并监督人类和动物组织免疫染色和组织阵列 制作。分子实验病理学核心联席主任傅阳新博士，医学博士，博士，指导， 小鼠组织银行，为小鼠组织的使用提供建议，为动物模型的使用和开发提供直接帮助， 特别是IBD相关疾病，并监督流式细胞仪服务的运作。

The Molecular Experimental Pathology Core of The University of Chicago DDRCC provides services vital to the study of intestinal disease, particularly immune-mediated, infectious, neoplastic and inflammatory bowel diseases, in human and animal tissues. In simplest terms, the core components can be broken into four major area:;: (1) human and animal (primarily mouse) tissue banking, (2) anatomic pathology review and consultation and tissue analysis (including immunohistochemistry, laser capture microdissection, tissue arrays), and (3) guidance in the use and design of animal nodels of intestinal disease and (4) flow cytometry. The Molecular Experimental Pathology Core was created through ,i reorganization of the Molecular Pathology Core in 2003 in an effort to better meet the needs of the DDRCC. The previous core provided training and direct services in specialized molecular techniques, genetic engineering of both iomatic and embryonic stem (ES) cell lines, and limited access to human tissues and genetic analysis of these tissues. However, due to restructuring of university core services during the previous funding interval, rbr example an excellent University of Chicago Transgenic/Knockout Mouse Core had been established, and advances in science, many of the specialized molecular techniques provided had become routine approaches easily accomplished in an individual investigator's lab or through other University of Chicago Cores, e.g. the DNA Sequencing Con;. A survey of DDRCC membership to determine the services that would best support and extend their work resulted ir a new focus on access to human and animal tissues, access to advanced tools, training in their use, expertise in data inrerpretation, and assistance in the use, rather than development, of mice. Where possible, the Molecular Experimental Pathology Core has partnered with existing resources to generate significant cost-savings to both the core and the DDRCC membership. The Administrative Director of the Molecular Experimental Pathology Core, Dr. Jerrold Turnet M.D., Ph.D., oversees the operations of all components and is directly involved in all human tissue-related operations, provides anatomic pathology review and consultation services, and supervises human and animal tissue immunostaining and tissue array production. The Co-Director of the Molecular Experimental Pathology Core, Dr. Yang-Xin Fu, M.D., Ph.D., directs, the mouse tissue bank, advises on mouse tissue use, provides direct assistance with use and development of animal models, particularly of IBD-related disease, and oversees the operation of flow cytometry services.