MOLECULAR AND EXPERIMENTAL PATHOLOGY CORE

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The Molecular Experimental Pathology Core of The University of Chicago DDRCC provides services vital to the study of intestinal disease, particularly immune-mediated, infectious, neoplastic and inflammatory bowel diseases, in human and animal tissues. In simplest terms, the core components can be broken into four major area:;: (1) human and animal (primarily mouse) tissue banking, (2) anatomic pathology review and consultation and tissue analysis (including immunohistochemistry, laser capture microdissection, tissue arrays), and (3) guidance in the use and design of animal nodels of intestinal disease and (4) flow cytometry. The Molecular Experimental Pathology Core was created through ,i reorganization of the Molecular Pathology Core in 2003 in an effort to better meet the needs of the DDRCC. The previous core provided training and direct services in specialized molecular techniques, genetic engineering of both iomatic and embryonic stem (ES) cell lines, and limited access to human tissues and genetic analysis of these tissues. However, due to restructuring of university core services during the previous funding interval, rbr example an excellent University of Chicago Transgenic/Knockout Mouse Core had been established, and advances in science, many of the specialized molecular techniques provided had become routine approaches easily accomplished in an individual investigator's lab or through other University of Chicago Cores, e.g. the DNA Sequencing Con;. A survey of DDRCC membership to determine the services that would best support and extend their work resulted ir a new focus on access to human and animal tissues, access to advanced tools, training in their use, expertise in data inrerpretation, and assistance in the use, rather than development, of mice. Where possible, the Molecular Experimental Pathology Core has partnered with existing resources to generate significant cost-savings to both the core and the DDRCC membership. The Administrative Director of the Molecular Experimental Pathology Core, Dr. Jerrold Turnet M.D., Ph.D., oversees the operations of all components and is directly involved in all human tissue-related operations, provides anatomic pathology review and consultation services, and supervises human and animal tissue immunostaining and tissue array production. The Co-Director of the Molecular Experimental Pathology Core, Dr. Yang-Xin Fu, M.D., Ph.D., directs, the mouse tissue bank, advises on mouse tissue use, provides direct assistance with use and development of animal models, particularly of IBD-related disease, and oversees the operation of flow cytometry services.