MOLECULAR BIOLOGY AND BIOCHEMISTRY CORE

DDRCC的分子生物学和生物化学（MBB）核心提供了对表征 所有生物体的基本组成部分：DNA、RNA、蛋白质和脂质。这些大分子的改变直接涉及表征胃肠道的免疫、炎症和肿瘤病症的调节障碍。该核心包括4个主要组件：（1）DNA，（2）RNA，（3）蛋白质和（4）膜脂质分析。该核心取代了膜生物物理学和生物化学核心，以响应DDRCC成员的调查，他们要求在这些生物分子领域的每个领域提供额外的基本分析服务。之前的几项服务，包括离子化Ca2+和免疫染色，被纳入其他核心。对于DNA分析，MBB与癌症中心测序设施合作，提高了效率和成本节约，并改进了DNA测序技术。核心的RNA组件包括真实的时间PCR和方便访问芝加哥大学的功能基因组学实验室。DDRCC与功能基因组学部门合作，在Xinininin Li博士的指导下，为DDRCC成员提供这种强大的技术，节省大量成本。核心的蛋白质组成部分包括方便进入蛋白质组学设施和信号转导研究的咨询服务。DDRCC与芝加哥大学蛋白质组学实验室合作，该实验室在Alex Schilling博士的指导下拥有最先进的质谱资源。DDRCC为成员提供蛋白质组学实验的大量折扣，以促进研究人员在GI相关疾病中更广泛地使用这种强大的技术。将原始核心的脂质组成研究扩展到包括膜脂筏制备物，以评估其作为信号平台的作用。核心组织的会计职能集中在行政核心组织，以提高效率、成本效益和与这些组成部分的协调。MBB核心的行政主任Marc Bissonnette博士和Judy Cho博士监督 所有组件。主任负责确保适当的科学指导和有效利用各自科室的服务和设施。对该核心进行重组，使成员能够以大大降低的成本使用DNA阵列和蛋白质组学分析，这增加了这些实验室的使用量，预计在本赠款的下一个周期内将大幅增长。由于该核心可节省大量费用、相关性以及高质量的服务和资源，因此被复员方案协调委员会成员大量使用。每个组成部分提供培训的新的和建立的研究人员不熟悉这些实验方法。MBB核心有助于促进多学科合作，并通过共享资源促进富有成效的交流。

The Molecular Biology and Biochemistry (MBB) Core of the DDRCC provides services critical to characterizing the fundamental building blocks of all living organisms: DNA, RNA, proteins and lipids. Alterations in these macromolecules are directly involved in dysregulations characterizing immune, inflammatory and neoplastic conditions of the Gl tract. This Core includes 4 major components: (1) DNA, (2) RNA, (3) Protein, and (4) Membrane lipid analysis. This Core replaced the Membrane Biophysics and Biochemistry Core in response to surveys of DDRCC members, who requested additional basic analytical services in each of these biomolecular areas. Several previous services, including ionized Ca2+ and immunostaining, were incorporated into other Cores. For DNA analysis, the MBB partnered with the Cancer Center equencing facility that enhanced the efficiency and cost savings and improved the technology for DNA sequencing. The Core's RNA component includes real time PCR and facilitated access to the University of Chicago's functional genomics laboratory. The DDRCC partnered with the functional genomics component, under the direction of Dr. Xininin Li, to offer this powerful technology at substantial cost savings to DDRCC members. The Core's protein component includes facilitated access to the proteomic facility and a consultative service for signal transduction studies. The DDRCC partnered with the University of Chicago proteomics laboratory that includes state of the art Mass Spectrometry resources under the direction of Dr. Alex Schilling. The DDRCC provides members substantial discounts for proteomics experiments to facilitate the wider use of this powerful technology by researchers in Gl-related disorders. The lipid composition stucies of the original Core were expanded to include membrane lipid raft preparations to assess their roles as signaling platforms. The Core's accounting functions are centralized in the administrative Core that enhances efficiency, cost-effectiveness, and coordination with these components. The Administrative Directors of the MBB Core, Drs. Marc Bissonnette and Judy Cho, oversee the operations of all components. Directors are responsible for ensuring proper scientific direction and efficient use of services and facilities of their respective sections. Restructuring this Core to allow members to use DNA arrays and proteomic analysis at greatly reduced costs has increased the usership of these laboratories, which is anticipated to grow substantially during the next cycle of this Grant. This Core is heavily used by DDRCC members because of substantial cost savings, relevance, and high quality of services and resources. Each of the Components offers training of new and established investigators unfamiliar with these experimental approaches. The MBB Core has helped to foster multidisciplinary collaborations ard promote productive exchanges brought about by sharing of resources.