MOLECULAR BIOLOGY CORE

分子生物学核心的目的是促进分子遗传学工具的应用 胃肠肽及其生理功能的研究。在所有方面，分子 生物核心功能提供基本服务并作为所有中心的教育资源 调查人员。尽管基本的分子技术可通过以下途径广泛提供给中心的研究人员 商业来源，仍然有特定的服务，最好是由汇集的资源和高度 训练有素的人员，如中心可以提供的人员。在上一个供资期间，有三个 提供了核心计划：转基因动物计划、病毒载体计划和基因 Expression程序。分子生物学核心增加了微阵列基因芯片计划，并已 本质上更新了基因表达计划，以提供有关以下技术的教育和培训 经过一定的理论和实践培训后，可以在研究人员自己的实验室里进行。 由于研究人员的需求可能已经超越了基本的基因表达方法，而涉及到 作为染色质结构和分析的技术，这个主要的教育项目已经更名为 分子技术培训计划。研究人员需要基础分子技术方面的帮助， 例如定量RT-PCR和更复杂的技术，例如染色质免疫沉淀分析 (CHIP)，可以接受核心董事(Merchant博士)和她的同事(BAI博士)的培训。

The purpose of the Molecular Biology core is to facilitate the application of the tools of molecular genetics to the study of gastrointestinal peptides and their physiological functions. In all aspects, the Molecular Biology Core functions to provide essential services and serves as an educational resource to all Center Investigators. Although basic molecular techniques are widely available to Center investigators through commercial sources, there remain specific services that are best provided by pooled resources and by highly trained personnel such as those that can be provided by a Center. During the prior funding period, three Core programs were provided: the Transgenic Animal Program, the Viral Vector Program and the Gene Expression Program. The Molecular Biology Core has added a Microarray Gene Chip Program and has essentially updated the Gene Expression Program to provide education and training in those techniques that can be performed in the investigators own laboratory after receiving some theoretical and practical training. Since investigator needs have likely moved beyond basic gene expression approaches to involve such techniques as chromatin structure and analysis, this primarily education program has been renamed the Molecular Techniques Training Program. Investigators needing assistance with basic molecular techniques, e.g., quantitative RT-PCR and more sophisticated techniques, e.g., chromatin immunoprecipitation assays (ChIP), can receive training from the Core Director (Dr. Merchant) and her associate (Dr. Bai).