Salmon fibrin gels for surgical applications

用于外科手术的鲑鱼纤维蛋白凝胶

• 批准号: 7281660
• 负责人: EVELYN SAWYER
• 金额: $ 36.85万
• 依托单位: SEA RUN HOLDINGS, INC.
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-05-15 至 2008-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7281660
• 关键词: Animal Model; Animals; Antibodies; Bandage; Binding; Biocompatible Materials; Brain Injuries; Cell Survival; Cells; Contusions; Craniocerebral Trauma; Dorsal; Excipients; Fibrin; Fibrinogen; Gamma Rays; Gel; Growth; Heating; Hemostatic Agents; Human; Inflammatory Response; Injury; Lesion; Liquid substance; Methods; Modeling; Neurons; Neurosurgeon; Operative Surgical Procedures; Patients; Percussion; Phase; Plasma; Property; Proteins; Public Health; Rattus; Salmon; Sampling; Site; Societies; Spinal Cord; Spinal cord injury; Testing; Thrombin; Tissues; United States Food and Drug Administration; Viral; Virus; Virus Inactivation; Work; axonal sprouting; central nervous system injury; cost; experience; fluid percussion injury; immunogenicity; in vivo; injured; neurotrophic factor; preclinical study

DESCRIPTION (provided by applicant): Our objective is to develop a new biomaterial, a salmon-derived fibrin gel, which will bridge injured CNS tissue, support and promote axonal sprouting and growth, and deliver neurotrophic factors and cells. In Aim #1 we will test the efficacy of salmon-derived fibrin gels in animal models of CNS injury. Using the rat dorsal hemi-section, contusion, and fluid percussion models, we will compare the salmon material to human-derived fibrin gels for efficacy, inflammatory response, and possible functional changes. In Aim #2 we will seek to identify the growth-promoting factors that are involved in the salmon gels. We will determine if there is a small active molecule bound to the fibrinogen, and what properties of the fibrinogen molecule are important. In Aim #3 we plan pre-clinical studies of viral clearance, immunogenicity, and stability that are necessary for FDA approval of salmon fibrinogen and thrombin. We will spike the proteins with virus, and determine combinations of heat and gamma radiation that will achieve viral reduction, preserve protein efficacy, and not result in immunogenicity. Tissues from treated rats (Aim #1) will be examined for inflammatory response, and plasma samples taken for antibody studies. The cost of spinal cord and brain injury in both dollars and human suffering is a major public health issue. If the neurosurgeon could intervene with a material that promotes neuronal regrowth and connectivity, the impacts for the patient and for society are enormous.

描述（由申请人提供）：我们的目标是开发一种新的生物材料，一种鲑鱼来源的纤维蛋白凝胶，它将桥接受伤的中枢神经系统组织，支持和促进轴突发芽和生长，并提供神经营养因子和细胞。在目标#1中，我们将测试鲑鱼来源的纤维蛋白凝胶在中枢神经系统损伤动物模型中的功效。使用大鼠背侧半切片、挫伤和液体冲击模型，我们将比较鲑鱼材料与人源纤维蛋白凝胶的功效、炎症反应和可能的功能变化。在目标#2 中，我们将寻求确定鲑鱼凝胶中涉及的生长促进因子。我们将确定是否存在与纤维蛋白原结合的小活性分子，以及纤维蛋白原分子的哪些特性是重要的。在目标 3 中，我们计划对病毒清除、免疫原性和稳定性进行临床前研究，这对于 FDA 批准鲑鱼纤维蛋白原和凝血酶是必要的。我们将用病毒刺入蛋白质，并确定热和伽马辐射的组合，以实现病毒减少、保留蛋白质功效且不会导致免疫原性。将检查接受治疗的大鼠的组织（目标#1）的炎症反应，并采集血浆样本用于抗体研究。脊髓和脑损伤造成的经济损失和人类痛苦是一个重大的公共卫生问题。如果神经外科医生能够使用促进神经元再生和连接的材料进行干预，那么对患者和社会的影响是巨大的。

项目成果

The potential for salmon fibrin and thrombin to mitigate pain subsequent to cervical nerve root injury.

鲑鱼纤维蛋白和凝血酶有可能减轻颈神经根损伤后的疼痛。

• DOI: 10.1016/j.biomaterials.2011.09.021
• 发表时间: 2011
• 期刊: Biomaterials
• 影响因子: 14
• 作者: Weisshaar,ChristineL;Winer,JessamineP;Guarino,BenjaminB;Janmey,PaulA;Winkelstein,BethA
• 通讯作者: Winkelstein,BethA