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Cytokinesis and RNA Segregation in Zebrafish Development

斑马鱼发育中的细胞分裂和 RNA 分离

基本信息

批准号：
7417653
负责人：
FRANCISCO J PELEGRI
金额：
$ 25.4万
依托单位：
UNIVERSITY OF WISCONSIN-MADISON
依托单位国家：
美国
项目类别：
财政年份：
2002
资助国家：
美国
起止时间：
2002-03-01 至 2008-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7417653
关键词：
Actins Address Affect Alleles Animals Aspartate Auras Biology Calcium Calcium Oscillations Cell Adhesion Cell Separation Cell division Cell physiology Cells Chromosome Mapping Classification Code Complex Coupled Cytokinesis Cytoplasm Cytoskeleton DNA Sequence Rearrangement Development Distal Embryo Event Exocytosis Genes Genetic Epistasis Genetic Transcription Germ Germ Cells Green Fluorescent Proteins Growth and Development function Image Island Lesion Life Link Localized Mediating Membrane Molecular Molecular Analysis Muscle Mutation Myosin Type II Organism Oxidoreductase Pathway interactions Pattern Pharmaceutical Preparations Process Proteins RNA Regulation Research Role Staging Transmembrane Transport Transport Process Zebrafish aspartate dehydrogenase aurora B kinase cell type daughter cell gene function insight intracellular protein transport knock-down mutant physical process protein expression protein localization location research study segregation

项目摘要

Cytokinesis, the physical process that divides daughter cells, is of fundamental importance to development and growth regulation. In many instances, cytokinesis is coupled with the asymmetric segregation of cellular determinants, which in turn results in the functional diversification of cell types. A basic question in biology is to understand the molecular mechanisms underlying the segregation of cell fate determinants, in particular the germ plasm, a specialized cytoplasm that confers the germ cell fate. Systematic screens for maternal-effect mutations in the zebrafish, Danio rerio, have identified genes that affect the processes of cytokinesis and germ plasm segregation, which in this organism are intimately linked. The gene aura appears to be involved in membrane and/or f-actin dynamics required for cell division and germ plasm recruitment, cellular island, which likely codes for the chromosomal passenger protein aurora B kinase, is involved in furrow initiation. The gene nebel, which likely encodes L-aspartate dehydrogenase is important for the amplification of calcium waves associated with furrow maturation and germ plasm compaction. In addition, we have identified a role for non-muscle myosin II in furrow maturation and germ plasm segregation. The proposed research will determine the precise roles of these genes and other subcellular events, such as cytoskeletal rearrangements, cortical transport and membrane exocytosis, within the coordinated pathway of events that drives cytokinesis and germ plasm segregation. The understanding of these processes will provide insights on a variety of poorly understood subjects: i) the events involved in cytokinesis, ii) the asymmetric segregation of germ plasm components within dividing cells, and iii) the functional relationship between the cellular mechanisms required for these two processes.

细胞质分裂，即子细胞分裂的物理过程，对发育至关重要