Laser Based 14C Counting for Biomedical Studies

用于生物医学研究的激光 14C 计数

• 批准号: 7185843
• 负责人: DANIEL E MURNICK
• 金额: $ 30.83万
• 依托单位: RUTGERS THE STATE UNIV OF NJ NEWARK
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7185843
• 关键词: Algorithms; Amino Acid Sequence; Biological; Biomedical Research; C14 isotope; Carbon Dioxide; Cells; Class; Complex; Count; Detection; Development; Devices; Diagnostic; Dose; Drug Kinetics; Electronics; Event; Gases; Image; Label; Laboratories; Lasers; Longitudinal Studies; Measurement; Measures; Medical; Medical Research; Methods; Noise; Nuclear Decay; Peptide Sequence Determination; Process; Radioactivity; Radioisotopes; Sampling; Signal Transduction; System; Techniques; Technology Transfer; Tracer; Variant; base; cost; digital; dosage; drug discovery; instrument; instrumentation; mass spectrometer; metabolic abnormality assessment; pressure; prototype; quantum; research facility; research study; seal; stable isotope; tool

DESCRIPTION (provided by applicant): The use of isotopic labeling with carbon 14 is widespread in biological and medical research, medical diagnostics and drug discovery and development. However, methods to detect radioisotopes depend on the detection of a nuclear decay event, an inefficient process as there is only 1 decay event per minute for every 4.35 billion atoms of 14C present in a sample, requiring the use of high levels of radioactivity. The aim of this project is to demonstrate a device, suitable for routine laboratory use, for atom counting of the tracer 14C, treating 14C as a "stable" isotope, decreasing the dosage required for most experiments and making new classes of studies possible. LARA will be extended from analysis of 13CO2 to similar analysis of 14CO2. Instrumentation will have sensitivity orders of magnitude greater than possible with scintillation (decay) detection and will compete with typical tandem accelerator mass spectrometers (AMS) that have demonstrated sensitivity at the picomole to the attomole level. The enhanced sensitivity is important for low dose and small sample tracer studies, long-term metabolic studies, pharmacokinetics studies and is being studied as a possible tool for protein sequencing and micro-imaging studies. A 14CO2 LARA device is projected to be considerably smaller, less complex and much lower in cost than an AMS with comparable capability. It will be shown that a sealed infrared laser operating at a unique infrared transition in 14CO2, can be routinely used to probe a sample cell containing carbon dioxide. The sample will be in a low pressure electrical discharge optimized for low noise detection of the optogalvanic effect. Such a system can be used to quantitatively measure small samples of 14C-enriched carbon dioxide. Results of the measurements with enriched samples will quantify improvements required to achieve ultimate sensitivity. Techniques will be developed to achieve enhanced sensitivity at the picomole to attomole level. Techniques will include electronic and digital algorithms to lower noise, gas mixture variations to enhance signal and quantum electronic (laser) enhancements. It is further aimed to build prototype instruments for routine laboratory use and transfer the technology to biomedical research facilities.

描述（由申请人提供）：