Development of on-chip ultra high-throughput whole-animal assay technologies
片上超高通量全动物检测技术的开发
基本信息
- 批准号:7431027
- 负责人:
- 金额:$ 251.75万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2007
- 资助国家:美国
- 起止时间:2007-09-30 至 2012-08-31
- 项目状态:已结题
- 来源:
- 关键词:AblationAccountingAddressAdultAdverse effectsAffectAfferent NeuronsAgeAgingAlgorithmsAlzheimer&aposs DiseaseAmericanAmyotrophic Lateral SclerosisAnestheticsAnimal ModelAnimalsAreaArtsAutomated Pattern RecognitionAutomobile DrivingAwardAxonAxotomyBackBehaviorBehavioralBehavioral AssayBiochemicalBiochemical GeneticsBioinformaticsBiologicalBiological AssayBiological ModelsBiological Neural NetworksBiological SciencesBiologyBiophotonicsBlood flowBrainC. elegans genomeCaenorhabditis elegansCandidate Disease GeneCell DeathCell LineCell membraneCellsCentral Nervous System DiseasesCentrifugationCessation of lifeChemical InjuryChemicalsClassCollaborationsColorCommunicationCommunitiesComplexComputational BiologyComputer softwareConditionConflict (Psychology)CuesCulture MediaCustomCyclic AMPDataData AnalysesDemyelinating DiseasesDendritesDetectionDevelopmentDifferentiation and GrowthDiffusionDimensionsDiseaseDisease modelDistalDoctor of PhilosophyDrug Delivery SystemsDyesElectrical EngineeringElectron MicroscopyElectronicsEmbryoEngineeringEnvironmentEnzymesEquipmentExposure toFacultyFeedbackFellowshipFigs - dietaryFluorescenceFlushingFundingFutureGene ClusterGene SilencingGene TargetingGenerationsGenesGeneticGenetic TechniquesGenomeGlassGoalsGrantGraphGray unit of radiation doseGreen Fluorescent ProteinsGrowthGrowth ConesGrowth FactorGuanosine Triphosphate PhosphohydrolasesHandHeadHealthcareHeatingHourHumanHuntington DiseaseHybridsHydrogelsImageImageryImaging TechniquesImmobilizationIncidenceIncubatorsIndividualInflammatoryInjuryInstitutesInternationalInterventionIntestinesInvasiveInvertebratesJournalsKnock-outKnowledgeLabelLasersLeadLeftLegal patentLengthLethal GenesLevamisoleLibrariesLifeLife ExpectancyLigandsLightLiquid substanceLongevityMainstreamingMammalsMapsMeasurableMeasurementMeasuresMechanicsMedicineMembraneMetalsMethodsMicrofluidic MicrochipsMicrofluidicsMicrospheresMicrosurgeryMindModelingMolecularMolecular and Cellular BiologyMonitorMorphologyMotionMotor Neuron DiseaseMotor NeuronsMovementMusMutagenesisMutationMyelinNanotechnologyNatural regenerationNatureNematodaNerveNerve DegenerationNerve RegenerationNervous system structureNeurobiologyNeurodegenerative DisordersNeurogliaNeuronsNew YorkNobel PrizeNoiseNuclearNumbersOperative Surgical ProceduresOpticsOrganismOrthologous GeneOutcomeOutputOxidopamineOxygenParkinson DiseaseParkinsonian DisordersPathway interactionsPatternPattern RecognitionPeripheral Nervous System DiseasesPersonsPhagocytosisPharmaceutical PreparationsPhenotypePhosphotransferasesPhysicsPhysiologic pulsePhysiologyPlasmaPoint MutationPolyaminesPopulationPositioning AttributePreclinical Drug EvaluationPreparationProblem SolvingProcessPropertyProtein OverexpressionProteinsPublicationsPulse takingPurposeRNA InterferenceRateRattusReaderReadingReagentRecoveryRecruitment ActivityRelative (related person)ReportingReproducibilityReproductionResearchResearch PersonnelResistanceResolutionResourcesResveratrolRight-OnRiskRoboticsRoleRole playing therapyRunningScanningScienceScientistScoreScreening procedureSensorySeriesShapesSideSignal PathwaySignal TransductionSiliconSmell PerceptionSolutionsSonSorting - Cell MovementSpace FlightSpeedSpinal CordStaining methodStainsStandards of Weights and MeasuresStereotypingStimulusStrokeStructureStudentsStudy modelsSuctionSurfaceSwimmingSynapsesSystemSystems BiologyTechniquesTechnologyTemperatureTestingTherapeutic InterventionThickTimeTitleTouch sensationToxic effectTransfectionTranslationsTraumatic Brain InjuryTubeUncertaintyUnited States National Institutes of HealthUniversitiesVariantVertebratesWallerian DegenerationWeekWorkYeastsZebrafishabsorptionabstractingage groupage relatedaxon growthaxon guidanceaxon regenerationaxonopathybasebehavior measurementcareercell motilitychemical releasecombinatorialcomputer sciencecostdaydensitydesigndesiredisabilitydosagedrug discoverydrug testingexperiencefluorescence imagingfunctional genomicsgain of functiongene functiongene interactiongenetic manipulationhigh throughput screeninghigh throughput technologyhuman RTN4 proteinhuman diseasehuman embryonic stem cellimage processingin vivoinhibitor/antagonistinjuredinnovationinsightinstrumentationinterestionizationkillingsknockout genemanmature animalmedical schoolsmicrochipmillisecondmortalitymotor neuron degenerationmulti-photonmutantmyelinationnanonanolitrenanoparticlenanoscalenanosurgerynerve stem cellnervous system disorderneural growthneurodevelopmentneuronal cell bodyneuronal growthneurotransmissionneurotrophic factornewsnoveloptical trapsparticlephotonicspreventprogramspromoterprotective effectprototypequantumreceptorrelating to nervous systemrelease factorresearch studyresponsesatisfactionscaffoldsizesmall moleculestem cellssuccesssupercomputersynaptic functionsynaptogenesistranscriptional coactivator p75translational medicinetrendtwo-photonultravioletwound healing
项目摘要
In recent years, the advantages of using the nematode Caenorhabditis elegans
as a model system for human disease have become increasingly apparent, culminating
in two Nobel Prizes in Physiology and Medicine within the last five years. Existing
vertebrate animal models, and the instrumentation incorporated to study them, cannot
be utilized for high throughput assays for rapid identification of novel genes, and drug
leads. The model organism C. elegans allows in vivo genome-wide assays and high-
throughput screens due to the availability of unmatched genetic techniques, its
transparency, and the ability to grow it in minute volumes. Yet, since the first publications
in early 1960s, little has changed how scientists manipulate this tiny organism manually,
as a result of which even simple large-scale assays still take months to years to
complete. Importantly, due to the lack of key technologies, several assays either cannot
be performed at all or have to be dramatically simplified for high-throughput screens.
We propose (1) development of the first on-chip ultra high-throughput whole-
animal manipulation technologies for in vivo drug screens and genome-wide discovery of
gene functions and interactions by complex on-chip behavioral and biochemical assay
strategies; and (2) the first large-scale in vivo study of mechanisms of neural
degeneration and regeneration following reproducible injuries using the proposed
techniques. The proposed technologies and high-throughput assay strategies can
significantly impact discovery of many molecular mechanisms using disease models of
small animals.
近年来,利用秀丽隐杆线虫的优势
作为人类疾病的模型系统已经变得越来越明显,
在过去的五年里,他两次获得诺贝尔生理学和医学奖。现有
脊椎动物模型,以及用于研究它们的仪器,不能
用于快速鉴定新基因和药物的高通量测定
线索.模式生物C. elegans允许在体内进行全基因组测定,
由于无与伦比的遗传技术的可用性,
透明度,以及在微小体积中生长的能力。然而,自从第一次出版以来,
在20世纪60年代早期,科学家们手动操纵这种微小生物方式几乎没有改变,
结果,即使是简单的大规模测定仍然需要数月至数年来
完成.重要的是,由于缺乏关键技术,几种检测方法要么不能
或者必须显著简化以用于高通量筛选。
我们建议(1)开发第一个片上超高通量全-
用于体内药物筛选和全基因组发现的动物操作技术
通过复杂的芯片上行为和生物化学测定的基因功能和相互作用
策略;(2)第一次大规模的神经机制的体内研究
退化和再生后,可再生的损伤,使用建议的
技术.所提出的技术和高通量测定策略可以
使用疾病模型发现许多分子机制,
小动物。
项目成果
期刊论文数量(13)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
High-throughput in vivo genetic and drug screening using femtosecond laser nano-surgery, and microfluidics.
使用飞秒激光纳米手术和微流体进行高通量体内遗传和药物筛选。
- DOI:10.1109/iembs.2008.4649743
- 发表时间:2008
- 期刊:
- 影响因子:0
- 作者:Rohde,Christopher;Gilleland,Cody;Samara,Chrysanthi;Zeng,Fei;Yanik,MehmetFatih
- 通讯作者:Yanik,MehmetFatih
Organ-targeted high-throughput in vivo biologics screen identifies materials for RNA delivery.
- DOI:10.1039/c4ib00150h
- 发表时间:2014-10
- 期刊:
- 影响因子:0
- 作者:Chang TY;Shi P;Steinmeyer JD;Chatnuntawech I;Tillberg P;Love KT;Eimon PM;Anderson DG;Yanik MF
- 通讯作者:Yanik MF
Microfluidic in vivo screen identifies compounds enhancing neuronal regeneration.
微流体体内筛选识别增强神经元再生的化合物。
- DOI:10.1109/iembs.2009.5334771
- 发表时间:2009
- 期刊:
- 影响因子:0
- 作者:Rohde,ChristopherB;Gilleland,Cody;Samara,Chrysanthi;Norton,Stephanie;Haggarty,Stephen;Yanik,MehmetFatih
- 通讯作者:Yanik,MehmetFatih
Fully automated cellular-resolution vertebrate screening platform with parallel animal processing.
- DOI:10.1039/c1lc20849g
- 发表时间:2012-02-21
- 期刊:
- 影响因子:6.1
- 作者:Chang TY;Pardo-Martin C;Allalou A;Wählby C;Yanik MF
- 通讯作者:Yanik MF
Large-scale analysis of neurite growth dynamics on micropatterned substrates.
微图案基底上神经突生长动力学的大规模分析。
- DOI:10.1039/c0ib00058b
- 发表时间:2011
- 期刊:
- 影响因子:0
- 作者:Wissner-Gross,ZacharyD;Scott,MarkA;Ku,David;Ramaswamy,Priya;FatihYanik,Mehmet
- 通讯作者:FatihYanik,Mehmet
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Mehmet Fatih Yanik其他文献
Functional regeneration after laser axotomy
激光轴突切断后的功能性再生
- DOI:
10.1038/432822a - 发表时间:
2004-12-15 - 期刊:
- 影响因子:48.500
- 作者:
Mehmet Fatih Yanik;Hulusi Cinar;Hediye Nese Cinar;Andrew D. Chisholm;Yishi Jin;Adela Ben-Yakar - 通讯作者:
Adela Ben-Yakar
Verfahren zur zelltransfektion mit nukleinsäuren
核转移的影响
- DOI:
- 发表时间:
2012 - 期刊:
- 影响因子:0
- 作者:
Mehmet Fatih Yanik;Matthew Angel - 通讯作者:
Matthew Angel
Deep-learning-based identification, tracking, pose estimation and behaviour classification of interacting primates and mice in complex environments
基于深度学习的复杂环境中相互作用的灵长类动物和小鼠的识别、跟踪、姿势估计和行为分类
- DOI:
10.1038/s42256-022-00477-5 - 发表时间:
2022-04-21 - 期刊:
- 影响因子:23.900
- 作者:
Markus Marks;Qiuhan Jin;Oliver Sturman;Lukas von Ziegler;Sepp Kollmorgen;Wolfger von der Behrens;Valerio Mante;Johannes Bohacek;Mehmet Fatih Yanik - 通讯作者:
Mehmet Fatih Yanik
Mehmet Fatih Yanik的其他文献
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{{ truncateString('Mehmet Fatih Yanik', 18)}}的其他基金
Generating transplantable neurons by in vivo combinatorial screening of transcrip
通过体内转录组合筛选产生可移植神经元
- 批准号:
8337690 - 财政年份:2011
- 资助金额:
$ 251.75万 - 项目类别:
Generating transplantable neurons by in vivo combinatorial screening of transcrip
通过体内转录组合筛选产生可移植神经元
- 批准号:
8142682 - 财政年份:2011
- 资助金额:
$ 251.75万 - 项目类别:
Generating transplantable neurons by in vivo combinatorial screening of transcrip
通过体内转录组合筛选产生可移植神经元
- 批准号:
8912552 - 财政年份:2011
- 资助金额:
$ 251.75万 - 项目类别:
Generating transplantable neurons by in vivo combinatorial screening of transcrip
通过体内转录组合筛选产生可移植神经元
- 批准号:
8508325 - 财政年份:2011
- 资助金额:
$ 251.75万 - 项目类别:
Generating transplantable neurons by in vivo combinatorial screening of transcrip
通过体内转录组合筛选产生可移植神经元
- 批准号:
8712586 - 财政年份:2011
- 资助金额:
$ 251.75万 - 项目类别:
HIGH-THROUGHPUT IN VIVO SUBCELLULAR-RESOLUTION VERTEBRATE SCREENING PLATFORM
高通量体内亚细胞分辨率脊椎动物筛选平台
- 批准号:
8268464 - 财政年份:2010
- 资助金额:
$ 251.75万 - 项目类别:
HIGH-THROUGHPUT IN VIVO SUBCELLULAR-RESOLUTION VERTEBRATE SCREENING PLATFORM
高通量体内亚细胞分辨率脊椎动物筛选平台
- 批准号:
8660716 - 财政年份:2010
- 资助金额:
$ 251.75万 - 项目类别:
HIGH-THROUGHPUT IN VIVO SUBCELLULAR-RESOLUTION VERTEBRATE SCREENING PLATFORM
高通量体内亚细胞分辨率脊椎动物筛选平台
- 批准号:
8477325 - 财政年份:2010
- 资助金额:
$ 251.75万 - 项目类别:
HIGH-THROUGHPUT IN VIVO SUBCELLULAR-RESOLUTION VERTEBRATE SCREENING PLATFORM
高通量体内亚细胞分辨率脊椎动物筛选平台
- 批准号:
8150903 - 财政年份:2010
- 资助金额:
$ 251.75万 - 项目类别:
HIGH-THROUGHPUT IN VIVO SUBCELLULAR-RESOLUTION VERTEBRATE SCREENING PLATFORM
高通量体内亚细胞分辨率脊椎动物筛选平台
- 批准号:
8016924 - 财政年份:2010
- 资助金额:
$ 251.75万 - 项目类别:
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