The Role of BDNF in Gustatory Neuron Development

BDNF 在味觉神经元发育中的作用

• 批准号: 7229583
• 负责人: Danielle E Harlow
• 金额: $ 2.76万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至 2008-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7229583
• 关键词: Address; Afferent Neurons; Ambystoma; Axon; Biological Assay; Brain-Derived Neurotrophic Factor; Cell Survival; Cells; Cephalic; Cessation of life; Computer Systems Development; Condition; Confocal Microscopy; Cranial Nerves; Data; Defect; Development; Ectoderm; Embryo; Embryonic Development; Epithelium; Event; Fellowship; Fluorescence; Ganglia; Genes; Green Fluorescent Proteins; In Vitro; Individual; Label; Location; Maps; Modality; Molecular Genetics; Mus; Myxoid cyst; Names; Nerve; Neural Crest; Neurologic; Neurons; Neurotrophic Tyrosine Kinase Receptor Type 2; Oral cavity; Peripheral; Population; Proteins; Research Training; Role; Salamander; Sensory Ganglia; Series; Signal Transduction; Source; Survival Analysis; System; Taste Buds; Taste Perception; Techniques; Testing; Time; Tissue Transplantation; Tongue; Transgenic Mice; Transplantation; Vertebrates; afferent nerve; base; design; ganglion cell; hindbrain; insight; nerve supply; neuroblast; neuron loss; neuronal survival; neurotrophic factor; placodal ectoderm; receptor; research study; segregation; sensory system; somatosensory; synaptogenesis

DESCRIPTION (provided by applicant): This application investigates the early embryonic events that underlie the development of sensory neurons that innervate the taste system. Specifically, it asks how neurons segregate to gustatory or non-gustatory (somatosensory) modalities. Cranial nerve ganglia that carry taste information arise embryonically from epibranchial placodes and the neural crest. Cell labeling and tissue transplantation techniques will be used to fate map placodes and crest to determine the embryonic origin of gustatory neurons. Gustatory neurons are dependent on brain derived neurotrophic factor (BDNF) for survival. When during development do gustatory neurons require BDNF and what is the source of BDNF required by gustatory neurons? These questions will be addressed using a molecular genetic approach, in which BDNF is selectively deleted from subsets of cranial ganglionic neurons in transgenic mice. The mechanisms involved in cranial nerve development are likely to be broadly involved in many aspects of early sensory system development. My results will lend basic insight into events of normal vertebrate embryogenesis and provide elementary data for the study of neurological defects.

描述（由申请人提供）：该应用研究了支配味觉系统的感觉神经元发育的早期胚胎事件。具体来说，它询问神经元如何分离到味觉或非味觉（体感）模态。携带味觉信息的脑神经节起源于胚胎时期的鳃上基板和神经嵴。细胞标记和组织移植技术将被用来命运地图基板和嵴，以确定味觉神经元的胚胎起源。味觉神经元的生存依赖于脑源性神经营养因子（BDNF）。在发育过程中，味觉神经元何时需要BDNF，味觉神经元所需的BDNF的来源是什么？这些问题将使用分子遗传学方法来解决，其中BDNF选择性地从转基因小鼠的颅神经节神经元亚群中删除。颅神经发育的机制可能广泛涉及早期感觉系统发育的许多方面。本研究结果将为了解正常脊椎动物胚胎发生的事件提供基本的认识，并为神经系统缺陷的研究提供基础数据。