Morphology & Physiology of Solitary Chemoreceptor Cells

形态学

• 批准号: 7209041
• 负责人: Brian D Gulbransen
• 金额: $ 1.15万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-03-20 至 2007-08-19
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7209041
• 关键词: ATP Receptors; Alcohol or Other Drugs use; Anterior; Apical; Apnea; Binding; Biological Assay; C Fiber; Calcitonin Gene-Related Peptide; Calcium; Caliber; Capsaicin; Cell Survival; Cell physiology; Cells; Characteristics; Chemoreceptors; Class; Data; Detection; Diffuse; Elements; Epithelial; Esthesia; Fellowship; Fiber; Fingers; GTP-Binding Proteins; Glutamate Receptor; Glutamates; Goals; Image; Individual; Inositol 1,4,5-Trisphosphate; Isolectin; Kainic Acid Receptors; Lectin; Ligands; Localized; Location; Mediating; Membrane; Morphology; Mus; Names; Nasal Epithelium; Nasal cavity; Nerve Endings; Nerve Fibers; Neuropeptides; Nociception; Nociceptors; Nose; Oral cavity; Pain; Phospholipase C; Physiological; Physiology; Process; Protein Isoforms; Range; Rattus; Receptor Cell; Signal Transduction; Sneezing; Stimulus; Structure of respiratory epithelium; Structure of trigeminal ganglion; Substance P; Surface; System; Taste Perception; Testing; Tight Junctions; Tissues; Trigeminal System; Trigeminal nerve structure; density; design; detector; falls; ganglion cell; gastrointestinal epithelium; glial cell-line derived neurotrophic factor; immunocytochemistry; irritation; kainate; nerve supply; neurotrophic factor; rat Gnat3 protein; receptor; research study; respiratory; response

DESCRIPTION (provided by applicant): Nasal trigeminal chemosensitivity in mice and rats is mediated in part by solitary chemoreceptor cells (SCCs) in the nasal epithelium. SCCs express the G-protein a-gustducin in addition to other elements of the "bitter"- taste signaling cascade including PLCp2, TrpM5 and T2R "bitter"-taste receptors. Many noxious substances elicit the sensation of bitter taste in the oral cavity but are irritating or painful in the nasal cavity. The proposed experiments fall into 2 aims: first to describe what type of trigeminal nociceptive fibers innervate SCCs. We will utilize immunocytochemistry to assay nerve fibers innervating SCCs for neuropeptide content, the ability to bind isolectin B4, and the expression of P2X3 and kainate receptors, both of which are commonly expressed by nociceptive fibers. Second, we will investigate which part of the SCC response is TrpM5-mediated. TrpMS is an obligatory component of T2R-mediated responses in taste cells and TrpM5- KO mice lack taste responses to T2R ligands. We will use calcium imaging to visualize SCC responses to test stimuli from a panel of classic bitter and trigeminal stimuli. Responses will be recorded in SCCs from both wild type and TrpM5-KO mice to separate TrpM5 and non-TrpM5-mediated responses.

描述（由申请方提供）：小鼠和大鼠的鼻三叉神经化学敏感性部分由鼻上皮中的孤立化学感受器细胞（SCC）介导。SCC表达G蛋白α-味觉蛋白以及“苦”味信号级联的其他元件，包括PLCp 2、TrpM 5和T2 R“苦”味受体。许多有毒物质在口腔中引起苦味的感觉，但在鼻腔中是刺激性的或疼痛的。本实验分为两个目的：首先描述哪种类型的三叉神经伤害感受纤维支配SCC。我们将利用免疫细胞化学来分析神经纤维支配SCC的神经肽含量，结合同工凝集素B4的能力，以及P2 X3和红藻氨酸受体的表达，这两者通常由伤害性纤维表达。其次，我们将研究哪部分SCC反应是TrpM 5介导的。TrpMS是味觉细胞中T2 R介导的应答的必需组分，并且TrpM 5- KO小鼠缺乏对T2 R配体的味觉应答。我们将使用钙成像来可视化SCC对来自一组经典苦味刺激和三叉神经刺激的测试刺激的反应。将在野生型和TrpM 5-KO小鼠的SCC中记录应答，以区分TrpM 5和非TrpM 5介导的应答。