Paclitaxel Carrier from Protein Library
Paclitaxel Carrier from Protein Library
批准号:
7128097
负责人:
Venita Gresham WATSON
金额:
$2.92万
依托单位国家:
美国
项目类别:
财政年份:
2006
资助国家:
美国
项目状态:
已结题
起止时间:
2006-01-01 至 2009-04-30
关键词:
AffinityAnionsBindingBinding ProteinsBiological AssayBiotinBlood VesselsCalorimetryCarbodiimidesCarboxylic AcidsCarrier ProteinsChimeric ProteinsCompetitive BindingComplexConvectionDialysis procedureDiffusionDissociationDrug CarriersDrug Delivery SystemsEndotheliumEthylene GlycolsFellowshipFoundationsGenerationsGenetic TranscriptionGoalsIn VitroIndividualLibrariesLocal HyperthermiaMeasuresMessenger RNAMethodsMolecular BiologyMovementNamesNormal Horse SerumPaclitaxelPermeabilityPharmaceutical PreparationsPhysical DialysisPolymerase Chain ReactionProteinsPuromycinRateRelative (related person)Reverse TranscriptionSerumSiteSolid NeoplasmSolubilitySolventsSystemTemperatureTitrationsTranslationsTumor TissueWateraqueousbasecarboxylateethylene glycolmacromoleculeneoplastic cellneutravidinpressureprotein degradationsmall moleculetooltumor
中文摘要
该项目的长期目标是制备一种基于蛋白质的药物载体,可用于药物
英文摘要
The long term objective of this project is to prepare a protein-based drug carrier that can be used in drug
targeting to solid tumors. Macromolecules are shown to accumulate in leaky blood vessels surrounding
rapidly growing tumor tissue. However, the movement of these molecules from where they are retained to
actual tumor cells is extremely slow, due to unfavorable convection, or solvent drag, around the tumor site. In
contrast, small molecules (<10 kDa)can utilize diffusion dictated by concentration gradient to access the site
of action. The overall goals of the proposed study are to: (1) synthesize water-soluble biotinylated derivative
of paclitaxel, (2) identify high-affinity paclitaxel-binding proteins via the mRNA display system, (3) further
select those that will release paclitaxel when pH is lowered to 6.5 or temperature is elevated to 42¿C, (4)
covalently couple these proteins to a dendrimer to increase the overall MW, and (5) finally characterize the
assembly in terms of paclitaxel binding and release.
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Paclitaxel Carrier from Protein Library
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批准号:7332286
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项目类别:
-
资助金额:$2.8万
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财政年份:2006
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负责人:Venita Gresham WATSON
-
依托单位:
Paclitaxel Carrier from Protein Library
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批准号:7558567
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项目类别:
-
资助金额:$3.0万
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财政年份:2006
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负责人:Venita Gresham WATSON
-
依托单位:
Paclitaxel Carrier from Protein Library
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批准号:7064999
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项目类别:
-
资助金额:$2.88万
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财政年份:2006
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负责人:Venita Gresham WATSON
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依托单位:
海外基金