Chromosome 6q and Diabetes In African Americans

非裔美国人的 6q 染色体与糖尿病

• 批准号: 7261253
• 负责人: Keith L Keene
• 金额: $ 0.72万
• 依托单位: WAKE FOREST UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-15 至 2007-11-19
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7261253
• 关键词: 6q24-q27; Address; Adipose tissue; Admixture; Affect; African American; Age; American; Biological; Biological Assay; Biological Process; Candidate Disease Gene; Cell Line; Chromosomes; Communities; Complications of Diabetes Mellitus; Data; Databases; Diabetes Mellitus; Disease; ESR1 gene; Epidemic; Estrogen Receptor alpha; Family; Family history of; Fellowship; Firefly Luciferases; Functional disorder; Gene Expression; Genes; Genetic; Genetic Polymorphism; Genotype; Goals; Haplotypes; Individual; Laboratories; Location; MCF7 cell; Methodology; Methods; Muscle Cells; Names; Non-Insulin-Dependent Diabetes Mellitus; Numbers; Obesity; Phenotype; Plasmids; Population; Population Control; Predisposition; RNA Splicing; Race; Reporter; Research; Risk Factors; Role; Single Nucleotide Polymorphism; Single Nucleotide Polymorphism Map; Techniques; Time; United States; Variant; base; caucasian American; diabetic; interest; islet; novel; positional cloning; vector

DESCRIPTION (provided by applicant): This application proposes to identify the gene(s) contributing to the 6q linkage peak that has been identified in African American families that have type 2 diabetes mellitus (T2DM). The positional cloning method will be used to evaluate and determine potential candidate genes in this region. We believe we have identified a novel diabetes gene in this region, Estrogen Receptor alpha (ESR1), which would represent the first diabetes gene positionally identified using data from an African American population. We aim to further explore single nucleotide polymorphisms and haplotypes that show association with T2DM in ESR1, as well as analyze SNPs in additional candidate genes, in order to determine if there is any association in those genes as well. Association findings will then be analyzed through functional assays to determine if there is the associated SNPs/haplotypes serve a biological function. Understanding how polymorphisms in ESR1, or any other candidate genes located under the 6q linkage peak, will provide critical information that will aid in understanding the pathophysiology of T2DM and how these variations contribute to the disease across different populations.

描述（由申请人提供）：本申请提出鉴定在患有2型糖尿病（T2 DM）的非裔美国人家族中鉴定的促成6 q连锁峰的基因。定位克隆方法将用于评估和确定该区域的潜在候选基因。我们相信我们已经在该区域确定了一种新的糖尿病基因，雌激素受体α（ESR 1），这将代表第一个使用非裔美国人数据定位确定的糖尿病基因。我们的目标是进一步探索ESR 1中与T2 DM相关的单核苷酸多态性和单倍型，以及分析其他候选基因中的SNP，以确定这些基因中是否也存在任何相关性。然后将通过功能测定分析关联结果，以确定是否存在相关的SNP/单倍型提供生物学功能。了解ESR 1或位于6 q连锁峰下的任何其他候选基因的多态性将提供关键信息，有助于了解T2 DM的病理生理学以及这些变异如何在不同人群中导致疾病。