Gradient patterned aECM proteins for cell studies

用于细胞研究的梯度图案 aECM 蛋白

• 批准号: 7231646
• 负责人: Stacey A Maskarinec
• 金额: $ 3.99万
• 依托单位: CALIFORNIA INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-06-01 至 2009-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7231646
• 关键词: Address; Behavior; Binding; Biocompatible Materials; Cells; Chemicals; Chemistry; Complex; Cues; Elastin; Endothelial Cells; Extracellular Matrix; Extracellular Matrix Proteins; Film; Mechanics; Microfluidics; Pattern; Preparation; Property; Proteins; RGD (sequence); Signal Transduction; Site; Surface; Tissues; Work; cell behavior; crosslink; design; desire; improved; in vivo; medical specialties; migration; novel; response

DESCRIPTION (provided by applicant): In vivo, cells integrate a complex set of physical and chemical signals as well as spatial gradients of these signals to maintain proper tissue function. There has been much progress in the artificial replication of these cellular microenvironments by producing substrates with either tailored surface chemistries or regions of varying mechanical compliance. Yet there has been minimal advancement in the design of materials that allow for the independent modulation of both properties to study cellular response. We propose to address this challenge by fabricating artificial extracellular matrix (aECM) films with superimposed gradients and observing cellular behavior on these surfaces. These unique aECM proteins are comprised of cell binding domains of RGD and CS5 and an elastin-derived repeat with a crosslinking site. Gradients in chemical cues can be controlled by the microfluidic mixing of different proteins while gradients in compliance can be achieved by varying the extent of crosslinking. Preparation of this specialty substrate provides a novel experimental approach for examining how competing signals generate a coordinated cellular response. Results from this work can also be applied to create improved materials for biomedical applications.

描述（由申请人提供）：在体内，细胞整合了一系列复杂的物理和化学信号以及这些信号的空间梯度，以维持适当的组织功能。通过生产具有定制表面化学或不同机械顺应性区域的底物，这些细胞微环境的人工复制已经取得了很大进展。然而，在允许独立调制这两种性质来研究细胞反应的材料设计方面，进展甚微。我们建议通过制造具有叠加梯度的人工细胞外基质（aECM）薄膜并观察这些表面上的细胞行为来解决这一挑战。这些独特的aECM蛋白由RGD和CS5的细胞结合结构域以及具有交联位点的弹性蛋白衍生重复序列组成。化学线索的梯度可以通过不同蛋白质的微流体混合来控制，而依从性的梯度可以通过改变交联的程度来实现。这种特殊底物的制备为研究竞争信号如何产生协调的细胞反应提供了一种新的实验方法。这项工作的结果也可以应用于创造用于生物医学应用的改进材料。