Cargo selectivity of the mammalian COPII coat complex

哺乳动物 COPII 外套复合物的货物选择性

• 批准号: 7492404
• 负责人: Joseph D Mancias
• 金额: $ 3.61万
• 依托单位: WEILL MEDICAL COLL OF CORNELL UNIV
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-03 至 2008-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7492404
• 关键词: Attention; Binding; Biochemical; Biological Assay; CCR5 gene; Caliber; Cell Surface Receptors; Cell physiology; Cellular biology; Complex; Endocytosis; Endoplasmic Reticulum; Ensure; Eukaryotic Cell; Exocytosis; Glycoproteins; Goals; Golgi Apparatus; Growth Factor; Hormones; In Vitro; Ion Channel; Lipids; Maintenance; Mammalian Cell; Maps; Mediating; Membrane; Methods; Molecular; N-Methyl-D-Aspartate Receptors; Neurotransmitters; Organelles; Protein Isoforms; Proteins; Research; Roentgen Rays; SNAP receptor; Signal Transduction; Vesicle; Vesicular stomatitis Indiana virus; chemokine receptor; inward rectifier potassium channel; syntaxin 5; trafficking

A hallmark of eukaryotic cells is the compartmentalization of cellular functions within membrane-enclosed organelles. Small (50-1 OOnm diameter) vesicles traffic between organelles transporting select cargo protein and lipid molecules. Selective transport ensures the maintenance of organellar composition and is the underlying mechanism of key cellular processes such as endocytosis, secretory function, and exocytosis of hormones and neurotransmitters. The COPII coat complex mediates the first step in secretory traffic by incorporating newly synthesized proteins into vesicles that bud from the endoplasmic reticulum (ER) and fuse with early Golgi compartments. The current proposal seeks to study the molecular determinants of cargo selection by the mammalian COPII complex using a combination of biochemical and X-ray crystallographic methods. Specifically, the proposal aims to study biologically important mammalian cargo molecules including: secreted growth factors, SNAREs, cell surface receptors, and membrane channels. The ultimate aim is to provide a molecular level description of all mammalian ER export motifs and their interaction with the COPII coat complex.

真核细胞的一个标志是细胞功能在膜封闭的细胞内的区室化。 细胞器小（50- 100 nm直径）囊泡在细胞器之间运输选择货物蛋白 和脂质分子。选择性运输确保了细胞器组成的维持， 关键细胞过程的潜在机制，如内吞作用、分泌功能和胞吐作用， 激素和神经递质。COPII被膜复合体介导分泌运输的第一步， 将新合成的蛋白质掺入从内质网（ER）出芽的囊泡中， 与早期的高尔基体融合目前的建议旨在研究的分子决定因素 使用生物化学和X射线的组合通过哺乳动物COPII复合物进行货物选择 晶体学方法具体而言，该提案旨在研究生物学上重要的哺乳动物货物 分子包括：分泌的生长因子、SNARE、细胞表面受体和膜通道。 最终的目的是提供所有哺乳动物ER输出基序的分子水平的描述和它们的功能。 与COPII涂层复合物的相互作用。