Cellular Redox Control and Oxidant Signaling

细胞氧化还原控制和氧化信号传导

基本信息

  • 批准号:
    7215550
  • 负责人:
  • 金额:
    $ 25.55万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    1999
  • 资助国家:
    美国
  • 起止时间:
    1999-06-01 至 2008-09-30
  • 项目状态:
    已结题

项目摘要

DESCRIPTION (provided by applicant): Partially reduced oxygen species are reactive derivatives of molecular oxygen well characterized to participate in cellular responses generally classified as oxidative stress. While the focus in the past has been on the role of reactive oxygen species (ROS) in toxicity and disease mechanisms, it has become increasingly clear that these molecules are powerful signaling molecules employed in normal cell growth and survival. The outcome of exposure/production of ROS depends on concentration and time course such that high, bolus exposure leads to toxicity (apoptosis or necrosis) while low continuous exposure enhances cell survival and stimulates growth. Recently several lines of evidence suggest that ROS produced by NAD(P)H oxidases (NOX) function in initiation and regulation of cell signaling pathways. For example, a NOX isoform appears to be activated by receptor tyrosine kinases (EGFR, PDGF, IR) leading to H202 production and increased phosphorylation of the receptor and enhanced signaling, inhibition of NOX activity in melanoma cells by antisense to NOX4 or p22phox leads to growth inhibition, and expression of NOX1 in fibroblasts leads to a transformed phenotype and tumor formation when these cells are injected in a mouse model. Further, elevated NOX expression is associated with smooth muscle cell proliferation and commonly seen in cells derived from human cancers. The mechanism through which NOX isoforms regulate growth and survival are not known. However, studies with exogenously applied H202 would suggest activation of growth and survival (antiapoptotic) signals are important. Thus, in these studies we will test the hypothesis that cellular expression of NOX isoforms regulates growth factor receptor activity and provides antiapoptotic signals leading to cell growth and survival. To accomplish this, we will manipulate NOX levels in cells in culture and determine the effects of altered expression on growth and apoptosis and investigate the underlying cellular mechanisms.
描述(由申请人提供):部分还原的氧物质是分子氧的活性衍生物,其特征在于参与通常归类为氧化应激的细胞反应。虽然过去的重点是活性氧 (ROS) 在毒性和疾病机制中的作用,但越来越清楚的是,这些分子是正常细胞生长和存活中使用的强大信号分子。 ROS 暴露/产生的结果取决于浓度和时间进程,因此高剂量暴露会导致毒性(细胞凋亡或坏死),而低连续暴露会增强细胞存活并刺激生长。最近的一些证据表明,NAD(P)H 氧化酶 (NOX) 产生的 ROS 在细胞信号传导途径的启动和调节中发挥作用。例如,NOX同种型似乎被受体酪氨酸激酶(EGFR、PDGF、IR)激活,导致H2O2产生、受体磷酸化增加和信号传导增强,通过反义NOX4或p22phox抑制黑色素瘤细胞中的NOX活性导致生长抑制,成纤维细胞中NOX1的表达导致表型转化和肿瘤 当这些细胞被注射到小鼠模型中时形成。此外,NOX 表达升高与平滑肌细胞增殖相关,并且常见于源自人类癌症的细胞中。 NOX 亚型调节生长和存活的机制尚不清楚。然而,外源施加H 2 O 2 的研究表明生长和存活(抗凋亡)信号的激活很重要。因此,在这些研究中,我们将检验这样的假设:NOX 同工型的细胞表达调节生长因子受体活性并提供导致细胞生长和存活的抗凋亡信号。为了实现这一目标,我们将操纵培养细胞中的 NOX 水平,确定表达改变对生长和凋亡的影响,并研究潜在的细胞机制。

项目成果

期刊论文数量(1)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Identification of protein nitrosothiols using phosphine-mediated selective reduction.
  • DOI:
    10.1016/j.niox.2011.11.001
  • 发表时间:
    2012-01-01
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Li S;Wang H;Xian M;Whorton AR
  • 通讯作者:
    Whorton AR
{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

A RICHARD WHORTON其他文献

A RICHARD WHORTON的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('A RICHARD WHORTON', 18)}}的其他基金

Regulation of Rho Signaling by S-Nitrosothiols
S-亚硝基硫醇对 Rho 信号传导的调节
  • 批准号:
    7748012
  • 财政年份:
    2009
  • 资助金额:
    $ 25.55万
  • 项目类别:
Regulation of Rho Signaling by S-Nitrosothiols
S-亚硝基硫醇对 Rho 信号传导的调节
  • 批准号:
    8235748
  • 财政年份:
    2009
  • 资助金额:
    $ 25.55万
  • 项目类别:
Regulation of Rho Signaling by S-Nitrosothiols
S-亚硝基硫醇对 Rho 信号传导的调节
  • 批准号:
    7997191
  • 财政年份:
    2009
  • 资助金额:
    $ 25.55万
  • 项目类别:
Regulation of Rho Signaling by S-Nitrosothiols
S-亚硝基硫醇对 Rho 信号传导的调节
  • 批准号:
    7580056
  • 财政年份:
    2009
  • 资助金额:
    $ 25.55万
  • 项目类别:
Cellular Redox Control and Oxidant Signaling
细胞氧化还原控制和氧化信号传导
  • 批准号:
    6876538
  • 财政年份:
    1999
  • 资助金额:
    $ 25.55万
  • 项目类别:
CELLULAR REDOX CONTROL AND NITRIC OXIDE SIGNALING
细胞氧化还原控制和一氧化氮信号传导
  • 批准号:
    2907116
  • 财政年份:
    1999
  • 资助金额:
    $ 25.55万
  • 项目类别:
Cellular Redox Control and Oxidant Signaling
细胞氧化还原控制和氧化信号传导
  • 批准号:
    6778412
  • 财政年份:
    1999
  • 资助金额:
    $ 25.55万
  • 项目类别:
CELLULAR REDOX CONTROL AND NITRIC OXIDE SIGNALING
细胞氧化还原控制和一氧化氮信号传导
  • 批准号:
    6184716
  • 财政年份:
    1999
  • 资助金额:
    $ 25.55万
  • 项目类别:
Cellular Redox Control and Oxidant Signaling
细胞氧化还原控制和氧化信号传导
  • 批准号:
    7031039
  • 财政年份:
    1999
  • 资助金额:
    $ 25.55万
  • 项目类别:
CELLULAR REDOX CONTROL AND NITRIC OXIDE SIGNALING
细胞氧化还原控制和一氧化氮信号传导
  • 批准号:
    6537477
  • 财政年份:
    1999
  • 资助金额:
    $ 25.55万
  • 项目类别:

相似海外基金

The earliest exploration of land by animals: from trace fossils to numerical analyses
动物对陆地的最早探索:从痕迹化石到数值分析
  • 批准号:
    EP/Z000920/1
  • 财政年份:
    2025
  • 资助金额:
    $ 25.55万
  • 项目类别:
    Fellowship
Animals and geopolitics in South Asian borderlands
南亚边境地区的动物和地缘政治
  • 批准号:
    FT230100276
  • 财政年份:
    2024
  • 资助金额:
    $ 25.55万
  • 项目类别:
    ARC Future Fellowships
The function of the RNA methylome in animals
RNA甲基化组在动物中的功能
  • 批准号:
    MR/X024261/1
  • 财政年份:
    2024
  • 资助金额:
    $ 25.55万
  • 项目类别:
    Fellowship
Ecological and phylogenomic insights into infectious diseases in animals
对动物传染病的生态学和系统发育学见解
  • 批准号:
    DE240100388
  • 财政年份:
    2024
  • 资助金额:
    $ 25.55万
  • 项目类别:
    Discovery Early Career Researcher Award
Zootropolis: Multi-species archaeological, ecological and historical approaches to animals in Medieval urban Scotland
Zootropolis:苏格兰中世纪城市动物的多物种考古、生态和历史方法
  • 批准号:
    2889694
  • 财政年份:
    2023
  • 资助金额:
    $ 25.55万
  • 项目类别:
    Studentship
Using novel modelling approaches to investigate the evolution of symmetry in early animals.
使用新颖的建模方法来研究早期动物的对称性进化。
  • 批准号:
    2842926
  • 财政年份:
    2023
  • 资助金额:
    $ 25.55万
  • 项目类别:
    Studentship
Study of human late fetal lung tissue and 3D in vitro organoids to replace and reduce animals in lung developmental research
研究人类晚期胎儿肺组织和 3D 体外类器官在肺发育研究中替代和减少动物
  • 批准号:
    NC/X001644/1
  • 财政年份:
    2023
  • 资助金额:
    $ 25.55万
  • 项目类别:
    Training Grant
RUI: Unilateral Lasing in Underwater Animals
RUI:水下动物的单侧激光攻击
  • 批准号:
    2337595
  • 财政年份:
    2023
  • 资助金额:
    $ 25.55万
  • 项目类别:
    Continuing Grant
RUI:OSIB:The effects of high disease risk on uninfected animals
RUI:OSIB:高疾病风险对未感染动物的影响
  • 批准号:
    2232190
  • 财政年份:
    2023
  • 资助金额:
    $ 25.55万
  • 项目类别:
    Continuing Grant
A method for identifying taxonomy of plants and animals in metagenomic samples
一种识别宏基因组样本中植物和动物分类的方法
  • 批准号:
    23K17514
  • 财政年份:
    2023
  • 资助金额:
    $ 25.55万
  • 项目类别:
    Grant-in-Aid for Challenging Research (Exploratory)
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了