CORE--Pathology

核心--病理学

• 批准号: 7297810
• 负责人: David Lagunoff
• 金额: $ 16.3万
• 依托单位: UNIV OF MED/DENT OF NJ-NJ MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7297810
• 关键词: animal tissue; biomedical facility; electron microscopy; histochemistry /cytochemistry; histology; histopathology; immunocytochemistry; molecular /cellular imaging; terminal nick end labeling; tissue /cell culture

Core E will provide all histological, histochemical, electron microscopic and immunohistochemical techniques required by the four projects. In addition core E will provide histopathological interpretations and quantitative image analyses on tissues and cultured cells as required by the projects. Two pathologists and a histotechnician comprise the core personnel. The two pathologists work closely with the project directors to assure the quality of the experimental results. The core operates 5 microscopes equipped for fluorescence and bright field microscopy and digital imaging for use of the core personnel and researchers from the projects. The core also maintains two confocal microscopes for detailed fluorescence studies. The repertory of stains includes H&E, trichrome, methenamine silver and picric acid Sirius red. The most often employed immunostains are for Kit and Ki-67. In addition the core provides a wide range of immunohistochemical procedures using diverse antibodies. TUNEL assays for presumptive apoptotic cells are routine in the core. Quantitative image analytic procedures include cell frequency, collagen assay by direct a real measurement and point counting for volume density measurements on gross, light microscopic and electron microscopic images.

核心E将提供所有组织学、组织化学、电子显微镜和免疫组织化学技术 四个项目的要求。此外，核心E将提供组织病理学解释和定量 根据项目需要对组织和培养细胞进行图像分析。两个病理学家和一个 组织技术人员构成核心人员。两位病理学家与项目负责人密切合作， 保证实验结果的质量。核心操作5台配备荧光的显微镜 以及明场显微镜和数字成像技术，供来自 项目核心还保持两个共聚焦显微镜进行详细的荧光研究。汇编 染色剂包括H&E、三色、乌洛托品银和苦味酸天狼星红。最常被雇用的 Kit和Ki-67的免疫染色。此外，核心提供了广泛的免疫组化 使用不同抗体的程序。推定凋亡细胞的TUNEL测定在核心中是常规的。 定量图像分析程序包括细胞频率，胶原含量通过直接真实的测量 和点计数，用于大体、光学显微镜和电子显微镜上的体积密度测量 图像.