CORE--Pathology

核心--病理学

• 批准号: 7297810
• 负责人: David Lagunoff
• 金额: $ 16.3万
• 依托单位: UNIV OF MED/DENT OF NJ-NJ MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7297810
• 关键词: animal tissue; biomedical facility; electron microscopy; histochemistry /cytochemistry; histology; histopathology; immunocytochemistry; molecular /cellular imaging; terminal nick end labeling; tissue /cell culture

Core E will provide all histological, histochemical, electron microscopic and immunohistochemical techniques required by the four projects. In addition core E will provide histopathological interpretations and quantitative image analyses on tissues and cultured cells as required by the projects. Two pathologists and a histotechnician comprise the core personnel. The two pathologists work closely with the project directors to assure the quality of the experimental results. The core operates 5 microscopes equipped for fluorescence and bright field microscopy and digital imaging for use of the core personnel and researchers from the projects. The core also maintains two confocal microscopes for detailed fluorescence studies. The repertory of stains includes H&E, trichrome, methenamine silver and picric acid Sirius red. The most often employed immunostains are for Kit and Ki-67. In addition the core provides a wide range of immunohistochemical procedures using diverse antibodies. TUNEL assays for presumptive apoptotic cells are routine in the core. Quantitative image analytic procedures include cell frequency, collagen assay by direct a real measurement and point counting for volume density measurements on gross, light microscopic and electron microscopic images.

Core E 将提供所有组织学、组织化学、电子显微镜和免疫组织化学技术 四个项目的要求。此外，核心 E 将提供组织病理学解释和定量 根据项目要求对组织和培养细胞进行图像分析。两名病理学家和一名 组织技术人员是核心人员。两位病理学家与项目主管密切合作， 保证实验结果的质量。核心运行 5 个配备荧光显微镜 以及供核心人员和研究人员使用的明场显微镜和数字成像 项目。该核心还配有两台共焦显微镜，用于详细的荧光研究。剧目 染色剂包括 H&E、三色、六胺银和苦味酸天狼星红。最常受雇的 免疫染色适用于 Kit 和 Ki-67。此外，核心还提供广泛的免疫组织化学检测 使用不同抗体的程序。推定凋亡细胞的 TUNEL 检测是核心常规检测。 定量图像分析程序包括细胞频率、通过直接实际测量进行胶原蛋白测定 以及用于总、光学显微镜和电子显微镜体积密度测量的点计数 图像。