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CORE--Pathology

核心--病理学

基本信息

批准号：
7673355
负责人：
David Lagunoff
金额：
$ 17.34万
依托单位：
UNIV OF MED/DENT OF NJ-NJ MEDICAL SCHOOL
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/7673355
关键词：
2-hydroxyethyl methacrylate Actins Acute Alcian Blue Alkaline Phosphatase Animals Annexins Anterior Antibodies Antigens Aorta Apoptosis Apoptotic Area Autopsy Avidin Binding Biological Assay Biotin Blood Vessels Body Weight Bone Marrow Brain Buffers Cannulas Capillary Endothelial Cell Cardiac Cardiac Myocytes Cell Count Cell Death Cell Nucleus Cell Size Cell membrane Cells Cessation of life Characteristics Chills Chloride Ion Chlorides Chromatin Cicatrix Citrate Citrates Collagen Collection Colony-Forming Units Assay Color Computer Workstations Computer software Condition Confusion Consult Coronary Occlusions Coronary Stenosis Coronary artery Coulter counter Count Cryoultramicrotomy Cytoplasm DAPI DNA DNA Nucleotidylexotransferase DNA Repair Data Denervation Depth Dextromethorphan Diffuse Dyes Electron Microscope Electrons Evaluation Exclusion Exhibits Family suidae Fatty acid glycerol esters Fibrosis Fixatives Fluorescein-5-isothiocyanate Fluorescence Formaldehyde Formalin Freezing Frequencies Genomics Glutaral Head Heart Heart Atrium Heating Hematoxylin and Eosin Staining Method Hibernation Histologic Histopathology Home environment Hour Housing Human Resources Image Image Analysis Immersion Investigative Technique Immunofluorescence Immunologic Immunohistochemistry In Situ In Situ Nick-End Labeling Incubated Individual Infarction Injection of therapeutic agent Instruction Ischemia Label Laboratories Lasers Left Left ventricular structure Length Ligase Light Liquid substance Location Magic Masks Measurement Measures Mediating Methacrylates Methenamine Methodology Methods Microscope Microscopic Microscopy Microspheres Microtomy Milk Mitochondria Mitochondrial Crista Modeling Modification Molecular Monastral blue dye Monoclonal Antibodies Mus Muscle Cells Myocardial Myocardial tissue Myocardium Necrosis Neurons New Brunswick Nitrogen Nuclear Numbers Oligonucleotides Optics Osmium Tetroxide Paraffin Paraffin Embedding Paraffin Tissue Participant Pathologist Pathology Perfusion Pericardial body location Phenols Phosphate Buffer Plant Resins Population Preparation Principal Investigator Procedures Process Program Research Project Grants Proliferating Propidium Diiodide Proteins Proto-Oncogene Protein c-kit Protocols documentation Quantitative Evaluations Range Rattus Reaction Reagent Reporter Research Personnel Retrieval Rhodamine Rhodamines Risk Role Saline Sampling Scanning Services Silver Methenamine Silver Staining Site Slice Slide Staining method Stains Standards of Weights and Measures Stem cells Sum Supervision Surface Sus scrofa Sympathectomy System TdT-Mediated dUTP Nick End Labeling Assay Techniques Temperature Tetrazolium Thick Time Tissue Banks Tissue Embedding Tissue Sample Tissues Tracer Trichrome stain method Tyrosine 3-Monooxygenase Value Meaning Ventricular Ventricular septum Viola Weight Width Work Writing Yang animal facility annexin A5 base caspase-3 cost density diaminobenzidine peroxidase digital imaging experience extracellular fluorescence imaging gel electrophoresis immunocytochemistry improved indexing injured interest interstitial interstitial cell isopentane kit protein light microscopy mean corpuscular volume observed microwave electromagnetic radiation morphometry neurofilament protein H paraform picric acid pressure programs research study response sample fixation size tetramethylrhodamine isothiocyanate tissue fixing tissue processing tissue/cell culture vascular bed

项目摘要

Core E will provide all histological, histochemical, electron microscopic and immunohistochemical techniques required by the four projects. In addition core E will provide histopathological interpretations and quantitative image analyses on tissues and cultured cells as required by the projects. Two pathologists and a histotechnician comprise the core personnel. The two pathologists work closely with the project directors to assure the quality of the experimental results. The core operates 5 microscopes equipped for fluorescence and bright field microscopy and digital imaging for use of the core personnel and researchers from the projects. The core also maintains two confocal microscopes for detailed fluorescence studies. The repertory of stains includes H&E, trichrome, methenamine silver and picric acid Sirius red. The most often employed immunostains are for Kit and Ki-67. In addition the core provides a wide range of immunohistochemical procedures using diverse antibodies. TUNEL assays for presumptive apoptotic cells are routine in the core. Quantitative image analytic procedures include cell frequency, collagen assay by direct a real measurement and point counting for volume density measurements on gross, light microscopic and electron microscopic images.

核心E将提供所有组织学、组织化学、电子显微镜和免疫组织化学技术四个项目的要求。此外，核心E将提供组织病理学解释和定量根据项目需要对组织和培养细胞进行图像分析。两个病理学家和一个组织技术人员构成核心人员。两位病理学家与项目负责人密切合作，保证实验结果的质量。核心操作5个显微镜配备荧光以及明场显微镜和数字成像技术，供来自项目核心还保持两个共聚焦显微镜进行详细的荧光研究。汇编染色剂包括H&E、三色、乌洛托品银和苦味酸天狼星红。最常被雇用的免疫染色是针对Kit和Ki-67的。此外，核心提供了广泛的免疫组化使用不同抗体的程序。推定凋亡细胞的TUNEL测定在核心中是常规的。定量图像分析程序包括细胞频率，胶原含量通过直接真实的测量和点计数，用于大体、光学显微镜和电子显微镜上的体积密度测量图像.