In vivo MRI of spinal cord lesions in EAE mice

EAE 小鼠脊髓损伤的体内 MRI

• 批准号: 7179290
• 负责人: MATTHEW D BUDDE
• 金额: $ 2.65万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-02-01 至 2009-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7179290
• 关键词: Autoimmune Diseases; Blood - brain barrier anatomy; Brain; Cells; Clinical; Demyelinations; Detection; Development; Diagnosis; Diagnostic; Diffusion Magnetic Resonance Imaging; Disease Progression; Disruption; Encephalomyelitis; Enhancing Lesion; Evaluation; Event; Exhibits; Experimental Autoimmune Encephalomyelitis; Gadolinium; Goals; Immune; Impairment; Infiltration; Inflammation; Investigation; Label; Lead; Lesion; Light; Magnetic Resonance Imaging; Modeling; Multiple Sclerosis; Multiple Sclerosis Lesions; Mus; Myelin; Neurologic; Neurologic Dysfunctions; Pathogenesis; Radial; Research; Spinal Cord; Spinal Cord Lesions; T-Lymphocyte; Techniques; Therapeutic; Time; Weight; Work; base; disability; in vivo; macrophage; mouse model; tool; white matter

DESCRIPTION (provided by applicant): Demyelinated lesions in the white matter of the brain and spinal cord are the pathological hallmark of the autoimmune disorder Multiple Sclerosis (MS). Active lesions, as observed in gadolinium (Gd) enhanced T1- weighted MRI, exhibit varying degrees of inflammation, demyelination, and axonal damage upon histological examination. The objective of the proposed study is to determine the time-course of inflammation, demyelination, and axonal damage and their contribution to lesion formation and progression. A longitudinal, in vivo investigation of the spinal cord in mice with experimental autoimmune encephalomyelitis (EAE), a model of MS, will be examined using MRI. Inflammation and breakdown of the blood-brain barrier will be assessed using magnetically loaded T-cells and Gd-enhancement, respectively (Aim 1). The extent of demyelination and axonal damage will be assessed using diffusion tensor imaging (Aim 2). All in vivo MRI findings will be correlated with neurological disability and validated with ex vivo MRI and histological analysis. The research will enhance the understanding of the dynamic pathological changes in EAE and MS and will assess the use of DTI as a diagnostic tool.

描述（由申请人提供）：脑和脊髓白色物质中的脱髓鞘病变是自身免疫性疾病多发性硬化症（MS）的病理标志。在钆（Gd）增强T1加权MRI中观察到的活动性病变在组织学检查时表现出不同程度的炎症、脱髓鞘和轴突损伤。本研究的目的是确定炎症、脱髓鞘和轴突损伤的时程及其对病变形成和进展的影响。将使用MRI检查患有实验性自身免疫性脑脊髓炎（EAE）（MS模型）的小鼠脊髓的纵向体内研究。将分别使用磁负载的T细胞和Gd增强来评估血脑屏障的炎症和破坏（目的1）。将使用扩散张量成像（Aim 2）评估脱髓鞘和轴突损伤的程度。所有体内MRI结果将与神经功能障碍相关，并通过离体MRI和组织学分析进行验证。该研究将提高对EAE和MS动态病理变化的理解，并将评估DTI作为诊断工具的使用。