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Differential Myocyte Response to Strain Vectors and Rate

肌细胞对应变向量和速率的差异反应

基本信息

批准号：
7277701
负责人：
SAMUEL SENYO
金额：
$ 3.09万
依托单位：
UNIVERSITY OF ILLINOIS AT CHICAGO
依托单位国家：
美国
项目类别：
财政年份：
2004
资助国家：
美国
起止时间：
2004-08-16 至 2008-05-15
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7277701
关键词：
Actins Behavior Biomechanics Cardiac Cardiac Myocytes Cell Count Cell Fractionation Cells Complex Condition Cues Cytoplasm Cytoskeleton Data Diamond Disease Endothelial Cells Ensure Environment Event Fibroblasts Focal Adhesion Kinase 1 Fractionation Frequencies Gene Expression Heart Human Individual Length Location Measures Mechanical Stimulation Mechanical Stress Mechanics Mediating Membrane Messenger RNA Microfilaments Modeling Muscle Cells Muscle Fibers Myocardial Myocardium Myosin ATPase Names Neonatal Nuclear Phosphorylation Play Production Protein Kinase C Protein translocation Proteins Rate Rattus Rest Rho-associated kinase Role Sampling Signal Transduction Signaling Molecule Skeletal Muscle Stimulus Stress Stretching Surface Testing Time Tissues Training Variant Western Blotting fiber cell immunocytochemistry in vivo novel protein activation response rho shear stress vector

项目摘要

DESCRIPTION (provided by applicant): Biomechanical forces play a dynamic role in cardiac muscle adaptation and disease. Mechanical deformation includes tensile, compressive, and shear stress. There is growing evidence that cells discriminate different vectors of mechanical stress and rate of application. However, the mechanism by which cells detect complex cyclic mechanical stimulus is poorly understood. Phosphorylation and translocation of proteins associated with the costamere or z-disc may mediate mechanotransduction of vector forces and rates of stress and include focal adhesion kinase (FAK), Rho kinase A (Rho), and protein kinase C (PKC). Insufficient data exists on the behavior of these proteins in cardiac muscle to mechanical strain vectors and even less in the response to strain application rates. Therefore, the hypothesis is that the direction and rate of mechanical strain regulates protein phosphorylation and localization in cardiac myocytes. Orienting cells will direct the delivery of strain vectors by plating rat neonatal cardiac myocytes on novel three-dimensional substrata. Western blotting and immunocytochemistry will evaluate phosphorylation and localization of FAK, Rho, and PKCepsilon to cyclic mechanical activity.

描述（由申请人提供）：生物力学力在心肌适应和疾病中起着动态作用。机械变形包括拉伸、压缩和剪切应力。越来越多的证据表明，细胞区分不同的载体的机械应力和应用率。然而，细胞检测复杂的周期性机械刺激的机制知之甚少。与肋节或Z盘相关的蛋白质的磷酸化和易位可以介导载体力和应激速率的机械转导，并且包括粘着斑激酶（FAK）、Rho激酶A（Rho）和蛋白激酶C（PKC）。关于心肌中这些蛋白质对机械应变矢量的行为的数据不足，关于对应变施加率的响应的数据更少。因此，假设是机械应变的方向和速率调节心肌细胞中的蛋白质磷酸化和定位。定向细胞将通过在新的三维基质上接种大鼠新生心肌细胞来指导应变载体的递送。蛋白质印迹和免疫细胞化学将评估FAK、Rho和PKC β的磷酸化和定位对循环机械活性的影响。