Transgenic Chickens using Primordial Germ Cells

使用原始生殖细胞的转基因鸡

• 批准号: 7382893
• 负责人: MARIE-CECILE VAN DE LAVOIR
• 金额: $ 3万
• 依托单位: ORIGEN THERAPEUTICS
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-03-01 至 2008-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7382893
• 关键词: Antibody Formation; Blood Circulation; Cells; Chickens; Chimera organism; Code; DNA; Deposition; Dyes; Embryo; Evaluation; Gene Targeting; Genes; Genetic; Genital system; Genome; Genomics; Germ Lines; Gland; Goals; Gonadal structure; Human; Injection of therapeutic agent; Knock-out; Modification; Monoclonal Antibodies; Phase; Population; Production; Protocols documentation; Purpose; Research Personnel; Route; Staging; Staining method; Stains; Structure of primordial sex cell; System; Technology; Therapeutic; Therapeutic Monoclonal Antibodies; Time; Tissues; Transfection; Transgenes; Transgenic Organisms; Tubular formation; base; day; egg; embryonic stem cell; genetically modified cells; mouse model; origen; polyclonal antibody; polyclonal human antibody; programs; stem; trafficking; transmission process

Origen Therapeutic's goal is to develop chickens that produce fully human monoclonal and polyclonal antibodies. To achieve this aim the chicken Origen needs the capability to insert and modify the chicken genome and to transmit these genomic modifications through the germline. Origen's platform centers around a cell based system that allows integration of large pieces ofDMA and allows precise deletions in the genome. To this end Origen has identified a cell population that has the potential to enter the germline. This application focuses on transfecting this cell population with large (150 kb) constructs and to knock-out specific genes within the chicken genome. These transfected cell popupations will then be trafficked through the germline to obtain a flock of transgenic chickens.

Origen Therapeutic的目标是培育出能产生完全人源单克隆抗体的鸡， 多克隆抗体。为了实现这一目标，鸡Origen需要插入和 修改鸡的基因组，并通过生殖系传递这些基因组修改。 Origen的平台以基于单元的系统为中心，该系统允许集成大片DMA 并允许基因组中的精确缺失。 为此，Origen已经确定了一个有潜力进入生殖系的细胞群。 本申请集中于用大（150kb）构建体转染该细胞群， 敲除鸡基因组中的特定基因。然后这些转染的细胞群体将 通过生殖系进行贩卖，以获得一群转基因鸡。