Transgenic Chickens using Primordial Germ Cells

ORIGEN治疗公司的目标是开发生产完全人类单克隆化和 多克隆抗体。为了实现这一目标，鸡肉Origen需要能够插入和 修改鸡的基因组，并通过生殖系传递这些基因组修改。 Origen的平台以基于蜂窝的系统为中心，该系统允许集成大型OFDMA 并允许基因组中的精确删除。 为此，Origen已经确定了一个有可能进入生殖系的细胞群体。 这项应用专注于用大的(150kb)构建体来转染这个细胞群体，并 敲除鸡基因组中的特定基因。然后，这些转基因细胞将弹出 通过种系被贩卖，以获得一群转基因鸡。

Origen Therapeutic's goal is to develop chickens that produce fully human monoclonal and polyclonal antibodies. To achieve this aim the chicken Origen needs the capability to insert and modify the chicken genome and to transmit these genomic modifications through the germline. Origen's platform centers around a cell based system that allows integration of large pieces ofDMA and allows precise deletions in the genome. To this end Origen has identified a cell population that has the potential to enter the germline. This application focuses on transfecting this cell population with large (150 kb) constructs and to knock-out specific genes within the chicken genome. These transfected cell popupations will then be trafficked through the germline to obtain a flock of transgenic chickens.